Detailed information of component protein


Summary

Component ID T6CP015348
Component protein type TssJ
T6SS ID (Type) T6SS01213 experimental (Type i3)
Strain Pseudomonas aeruginosa PAK
Replicon chromosome
Sequence Protein sequence (155 a.a.); Nucleotide sequence (465 bp)
Reference
[1] Chen R, Weng Y, Zhu F, et al. Polynucleotide Phosphorylase Regulates Multiple Virulence Factors and the Stabilities of Small RNAs RsmY/Z in Pseudomonas aeruginosa. Front Microbiol. 2016 Mar 2;7:247. doi: 10.3389/fmicb.2016.00247. eCollection 2016. PMID: 26973625
[2] Li K, Xu C, Jin Y, et al. SuhB is a regulator of multiple virulence genes and essential for pathogenesis of Pseudomonas aeruginosa. mBio. 2013 Oct 29;4(6):e00419-13. doi: 10.1128/mBio.00419-13. PMID: 24169572
[3] Brencic A, Lory S. Determination of the regulon and identification of novel mRNA targets of Pseudomonas aeruginosa RsmA. Mol Microbiol. 2009 May;72(3):612-32. doi: 10.1111/j.1365-2958.2009.06670.x. PMID: 19426209
[4] Marden JN, Diaz MR, Walton WG, et al. An unusual CsrA family member operates in series with RsmA to amplify posttranscriptional responses in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2013 Sep 10;110(37):15055-60. doi: 10.1073/pnas.1307217110. Epub 2013 Aug 26. PMID: 23980177
[5] Aragon IM, Pérez-Mendoza D, Moscoso JA, et al. Diguanylate cyclase DgcP is involved in plant and human Pseudomonas spp. infections. Environ Microbiol. 2015 Nov;17(11):4332-51. doi: 10.1111/1462-2920.12856. Epub 2015 Apr 28. PMID: 25809128
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) Y880_RS27010
Coordinate (Strand) 5676992..5677456 (-)
NCBI ID WP_003083660.1
RefSeq NZ_CP020659
Uniprot ID V6A7H4_PSEAI, A0A1C7BPS2_PSEAI, A0A0H2ZJR0_PSEAB, Q9I752_PSEAE, A0A6N0KT96_9PSED, A0A069QCR5_PSEAI
KEGG ID pae:PA0080, pau:PA14_00960
PDB ID 3ZHN
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS-SciN PF12790.9 8.8e-41 27..143
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-154 MQKLIVALFGLLLAACSSSPPETPPTRVVIWLHAAPNLNPSAAGQAAPLRLRLYELKKDTAFGRADYFALTDNAQSTLGGDLVEQDEFLLRPGEERRIERTLDEQTRQLGFVAAYRDLDRATWRQVLDVPGQRTSHLDITLGAQAIGIVARPAP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.9992 pos: 15-16. LAA-CS. MQKLIVALFGLLLAA


  Protein sequence: 155 a.a.    

>T6CP015348 NZ_CP020659:c5677456-5676992 [Pseudomonas aeruginosa PAK] [TssJ]
MQKLIVALFGLLLAACSSSPPETPPTRVVIWLHAAPNLNPSAAGQAAPLRLRLYELKKDTAFGRADYFALTDNAQSTLGG
DLVEQDEFLLRPGEERRIERTLDEQTRQLGFVAAYRDLDRATWRQVLDVPGQRTSHLDITLGAQAIGIVARPAP*

  Nucleotide sequence: 465 bp    

>T6CP015348 NZ_CP020659:c5677456-5676992 [Pseudomonas aeruginosa PAK] [TssJ]
ATGCAGAAACTGATCGTCGCGCTGTTCGGGCTGCTGCTCGCCGCCTGTTCGTCCAGCCCGCCGGAAACCCCGCCGACCCG
GGTGGTGATCTGGCTCCACGCCGCACCGAACCTCAACCCGTCCGCCGCCGGCCAGGCGGCGCCGCTGCGCCTGCGGCTGT
ACGAGCTGAAGAAAGACACCGCGTTCGGCCGCGCCGACTACTTCGCCCTCACCGACAACGCCCAGTCGACCCTCGGCGGC
GACCTGGTGGAGCAGGACGAGTTCCTCCTGCGTCCGGGCGAGGAACGGCGCATCGAACGCACGCTCGACGAGCAGACCCG
CCAGCTCGGCTTCGTCGCCGCCTACCGCGACCTTGACCGCGCCACCTGGCGCCAGGTCCTCGACGTGCCGGGGCAACGCA
CCAGCCATCTCGACATCACGCTCGGCGCGCAGGCCATCGGCATCGTCGCGCGTCCCGCGCCCTGA