Detailed information of component protein


Summary

Component ID T6CP015346
Component protein type TssM
T6SS ID (Type) T6SS01213 experimental (Type i3)
Strain Pseudomonas aeruginosa PAK
Replicon chromosome
Sequence Protein sequence (1178 a.a.); Nucleotide sequence (3534 bp)
Reference
[1] Chen R, Weng Y, Zhu F, et al. Polynucleotide Phosphorylase Regulates Multiple Virulence Factors and the Stabilities of Small RNAs RsmY/Z in Pseudomonas aeruginosa. Front Microbiol. 2016 Mar 2;7:247. doi: 10.3389/fmicb.2016.00247. eCollection 2016. PMID: 26973625
[2] Li K, Xu C, Jin Y, et al. SuhB is a regulator of multiple virulence genes and essential for pathogenesis of Pseudomonas aeruginosa. mBio. 2013 Oct 29;4(6):e00419-13. doi: 10.1128/mBio.00419-13. PMID: 24169572
[3] Brencic A, Lory S. Determination of the regulon and identification of novel mRNA targets of Pseudomonas aeruginosa RsmA. Mol Microbiol. 2009 May;72(3):612-32. doi: 10.1111/j.1365-2958.2009.06670.x. PMID: 19426209
[4] Marden JN, Diaz MR, Walton WG, et al. An unusual CsrA family member operates in series with RsmA to amplify posttranscriptional responses in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2013 Sep 10;110(37):15055-60. doi: 10.1073/pnas.1307217110. Epub 2013 Aug 26. PMID: 23980177
[5] Aragon IM, Pérez-Mendoza D, Moscoso JA, et al. Diguanylate cyclase DgcP is involved in plant and human Pseudomonas spp. infections. Environ Microbiol. 2015 Nov;17(11):4332-51. doi: 10.1111/1462-2920.12856. Epub 2015 Apr 28. PMID: 25809128
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) Y880_RS26995
Coordinate (Strand) 5670794..5674327 (-)
NCBI ID WP_016252830.1
RefSeq NZ_CP020659
Uniprot ID UPI000339164E
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 4.9e-99 195..452
IcmF-related PF06761.14 3e-86 504..797
IcmF_C PF06744.14 2.7e-28 1052..1158
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-6 MKGFLN
Transmembrane helix 7-29 FFARWVVPVLGLLALSLIIWFLG
Outside 30-43 PLLRLGNYEPLASA
Transmembrane helix 44-66 TSRWVLIVLLFLVWIGFRVLRIV
Inside 67-444 QARRNAAKVMQSLAEVSAPDAASVATAEELATLKQRMDEALALLKRAKLGGSERRNLYELPWYVIIGPPGSGKTTALMNSGLDFPLAAQMGAGAIRGVGGTRNCDWWFTDEAVLLDTAGRYTTQDSHAQVDKAAWLGFLDLLKTQRKRRPIDGAFIAISLSDLLLGSDAERAAHAQAIRARIQELYQQLGVRFPIYVMLTKFDLVPGFMEFFDSLNREERAQVWGMTFALDDGKSAEGPLAVFDSEFALLEQRLTARLVERLQQERDPARRDLVYGFPQQFAALRECLGEFLNGVFKPNPYEERPLLRGLYFTSGTQEGSPIDRLIGSMAQSMNLDRQHLARQTGTGRSYFIERLFREVAFGERGLVGTNPKVERRRK
Transmembrane helix 445-467 WLTIGALSATALVVLAVTAVWIA
Outside 468-1177 SYRANQSYIAAVDQRVDPLARGIESLSPAQRDVLAVLPQLNAVQNLAGDAPSWAEGYGLYQGDMLGEESASVYRKLLIAVFAPRLVTRIEEQLRSGGSSDFLYEGLKAYLMLGSPDHYDADFIKAWISLDWERNLPRDLSPEQRQALHAHLDALLERRPPSARLDQDLVEDLRRQLQQLPVAQRVYDRVKRQRLPKDVPDFRISDAAGRDAPLVFARKSGKPLTDPLSGFFTYRGYREVFLTASLSQAGTIAEEQWVLGRDLNDAGDAANLALDVRRLYFQDYLRQWDDLLADLTVVPITNVTQAADVLRILSGPTSPFRKLLEAVARETDLQKGDRLVAAQVKKAADGTVDKLKQRLGSLVGQEEEEGAREQPQQADSDPISAHFAELNSLVSKGEGGNEPAPIDSLLEDMNALYVQVSAMAGASGDSLLGDAKNQVAAAASRVALSAERQPPVVQGLVKNVVNSTTSSMMGSVRNQLNAAWISDVVSVYRQSLAGRYPIAAGSSRDATLEDFGHFFGAGGVMDSYFRQYLQPYVDTSASTWRWQPGAAQKLGINPGVLHTFQRAAAIRDAFFRSGGMQPTVRFELKPVTMDAAISQFILDLDGQQLTYDHGPSRPVAMQWPSANGLGVVRLTVTPPPSSGRSGLTLEGPWAWFRLLDQSDLERGNSPDRFTLRLRIDGSSIACELRASSAFNPFKSRVVSGFSLPERL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9969 - -


  Protein sequence: 1178 a.a.    

>T6CP015346 NZ_CP020659:c5674327-5670794 [Pseudomonas aeruginosa PAK] [TssM]
MKGFLNFFARWVVPVLGLLALSLIIWFLGPLLRLGNYEPLASATSRWVLIVLLFLVWIGFRVLRIVQARRNAAKVMQSLA
EVSAPDAASVATAEELATLKQRMDEALALLKRAKLGGSERRNLYELPWYVIIGPPGSGKTTALMNSGLDFPLAAQMGAGA
IRGVGGTRNCDWWFTDEAVLLDTAGRYTTQDSHAQVDKAAWLGFLDLLKTQRKRRPIDGAFIAISLSDLLLGSDAERAAH
AQAIRARIQELYQQLGVRFPIYVMLTKFDLVPGFMEFFDSLNREERAQVWGMTFALDDGKSAEGPLAVFDSEFALLEQRL
TARLVERLQQERDPARRDLVYGFPQQFAALRECLGEFLNGVFKPNPYEERPLLRGLYFTSGTQEGSPIDRLIGSMAQSMN
LDRQHLARQTGTGRSYFIERLFREVAFGERGLVGTNPKVERRRKWLTIGALSATALVVLAVTAVWIASYRANQSYIAAVD
QRVDPLARGIESLSPAQRDVLAVLPQLNAVQNLAGDAPSWAEGYGLYQGDMLGEESASVYRKLLIAVFAPRLVTRIEEQL
RSGGSSDFLYEGLKAYLMLGSPDHYDADFIKAWISLDWERNLPRDLSPEQRQALHAHLDALLERRPPSARLDQDLVEDLR
RQLQQLPVAQRVYDRVKRQRLPKDVPDFRISDAAGRDAPLVFARKSGKPLTDPLSGFFTYRGYREVFLTASLSQAGTIAE
EQWVLGRDLNDAGDAANLALDVRRLYFQDYLRQWDDLLADLTVVPITNVTQAADVLRILSGPTSPFRKLLEAVARETDLQ
KGDRLVAAQVKKAADGTVDKLKQRLGSLVGQEEEEGAREQPQQADSDPISAHFAELNSLVSKGEGGNEPAPIDSLLEDMN
ALYVQVSAMAGASGDSLLGDAKNQVAAAASRVALSAERQPPVVQGLVKNVVNSTTSSMMGSVRNQLNAAWISDVVSVYRQ
SLAGRYPIAAGSSRDATLEDFGHFFGAGGVMDSYFRQYLQPYVDTSASTWRWQPGAAQKLGINPGVLHTFQRAAAIRDAF
FRSGGMQPTVRFELKPVTMDAAISQFILDLDGQQLTYDHGPSRPVAMQWPSANGLGVVRLTVTPPPSSGRSGLTLEGPWA
WFRLLDQSDLERGNSPDRFTLRLRIDGSSIACELRASSAFNPFKSRVVSGFSLPERL*

  Nucleotide sequence: 3534 bp    

>T6CP015346 NZ_CP020659:c5674327-5670794 [Pseudomonas aeruginosa PAK] [TssM]
ATGAAAGGCTTCCTCAACTTCTTCGCGCGCTGGGTGGTGCCGGTGCTCGGCCTGCTCGCCCTGAGCCTGATCATCTGGTT
CCTCGGCCCGCTGCTGCGCCTGGGCAACTACGAACCGCTGGCCTCGGCGACCAGCCGCTGGGTGCTGATCGTCCTGCTGT
TCCTGGTCTGGATCGGCTTCCGCGTGCTGCGCATCGTGCAGGCCCGGCGCAACGCGGCGAAGGTCATGCAGAGTCTCGCC
GAGGTCAGCGCGCCGGACGCCGCCAGCGTCGCCACCGCCGAAGAGCTGGCGACCCTCAAGCAACGCATGGACGAGGCCCT
GGCCCTGCTCAAGCGGGCCAAGCTGGGCGGCAGCGAGCGGCGCAACCTGTACGAGTTGCCCTGGTACGTGATCATCGGCC
CGCCCGGTTCGGGCAAGACCACCGCGCTGATGAATTCCGGCCTGGACTTCCCGCTCGCCGCACAGATGGGCGCCGGGGCG
ATCCGCGGGGTCGGCGGCACGCGCAACTGCGACTGGTGGTTCACCGACGAAGCGGTGCTGCTGGACACCGCCGGCCGCTA
TACCACCCAGGACAGCCACGCCCAGGTGGACAAGGCCGCCTGGCTGGGCTTCCTCGACCTGCTCAAGACCCAGCGCAAGC
GCCGCCCCATCGATGGCGCGTTCATCGCCATCAGCCTTTCCGACCTGCTGCTGGGCAGCGACGCCGAGCGCGCCGCCCAT
GCCCAGGCGATCCGCGCACGCATCCAGGAGCTGTACCAGCAGCTCGGGGTGCGCTTCCCGATCTACGTGATGCTGACCAA
GTTCGACCTGGTGCCGGGCTTCATGGAGTTCTTCGACAGCCTGAACCGCGAGGAACGCGCGCAGGTCTGGGGGATGACCT
TCGCCCTCGACGACGGCAAGAGCGCGGAAGGCCCGCTGGCGGTGTTCGACAGCGAATTCGCCCTGCTCGAACAGCGTCTC
ACCGCGCGCCTGGTGGAACGCCTGCAGCAGGAGCGCGACCCGGCGCGGCGCGACCTGGTCTACGGCTTCCCGCAGCAATT
CGCCGCCCTGCGCGAATGCCTCGGCGAGTTCCTCAACGGCGTGTTCAAGCCCAACCCCTACGAGGAACGCCCGCTGTTGC
GCGGCCTGTATTTCACCAGCGGAACCCAGGAAGGCAGTCCGATCGACCGCCTGATCGGCAGCATGGCGCAGTCGATGAAC
CTCGACCGCCAGCACCTGGCGCGGCAGACCGGAACCGGGCGCAGCTACTTCATCGAGCGGCTGTTCCGCGAAGTGGCATT
CGGCGAACGCGGCCTGGTCGGCACCAACCCGAAGGTCGAGCGCCGCCGCAAGTGGCTGACCATCGGCGCGCTGAGCGCCA
CCGCGCTGGTGGTGCTGGCGGTGACGGCGGTATGGATCGCCAGCTACCGCGCCAACCAGAGCTACATCGCCGCGGTCGAC
CAGCGCGTCGACCCGCTGGCGCGCGGCATCGAGTCGCTGAGCCCGGCCCAGCGCGACGTCCTGGCCGTACTGCCACAGCT
CAACGCGGTGCAGAATCTCGCCGGCGACGCGCCGAGCTGGGCCGAAGGCTACGGGCTGTACCAGGGCGACATGCTCGGCG
AGGAGTCCGCCAGCGTCTACCGCAAGCTGCTGATCGCGGTGTTCGCCCCGCGCCTGGTGACCCGCATCGAGGAACAGTTG
CGCTCCGGCGGCAGCAGCGATTTCCTCTACGAGGGCCTGAAGGCCTACCTGATGCTCGGCAGCCCGGACCATTACGACGC
CGACTTCATCAAGGCCTGGATCAGCCTCGACTGGGAACGCAACCTGCCGCGCGACCTCTCGCCGGAACAGCGCCAGGCGC
TGCACGCGCACCTCGACGCGTTGCTCGAACGACGGCCGCCGTCGGCGCGCCTGGACCAGGACCTGGTCGAGGACCTGCGC
CGCCAGTTGCAGCAACTGCCGGTGGCGCAGCGGGTCTACGACCGGGTCAAGCGGCAGAGGCTGCCCAAGGACGTGCCCGA
CTTCCGCATCAGCGACGCCGCCGGCCGCGACGCGCCGCTGGTGTTCGCGCGCAAGAGCGGCAAGCCGCTCACCGACCCGC
TCAGCGGCTTCTTCACCTACCGCGGCTATCGCGAGGTGTTCCTCACCGCCAGCCTCAGCCAGGCCGGCACCATCGCCGAG
GAACAGTGGGTGCTCGGGCGCGACCTGAACGATGCCGGCGACGCCGCCAACCTGGCCCTCGACGTGCGTCGCCTGTATTT
CCAGGACTACCTGCGGCAGTGGGACGACCTGCTCGCCGACCTCACCGTGGTGCCGATCACCAATGTCACCCAGGCCGCCG
ACGTGCTGCGCATTCTCTCCGGACCGACCTCGCCGTTCCGCAAGCTGCTCGAGGCAGTGGCCCGCGAGACCGACCTGCAG
AAGGGCGACCGGCTGGTCGCCGCGCAAGTGAAGAAGGCCGCCGACGGCACCGTGGACAAGCTCAAGCAGCGCCTCGGCTC
GCTGGTCGGCCAGGAGGAGGAGGAAGGCGCCCGCGAACAGCCGCAGCAGGCCGACAGCGACCCGATCAGCGCCCACTTCG
CCGAACTCAACAGCCTGGTCAGCAAGGGCGAGGGCGGCAACGAGCCGGCGCCGATCGACAGCCTGCTGGAAGACATGAAC
GCGCTCTACGTGCAGGTCAGCGCCATGGCTGGGGCCAGCGGCGACTCGCTGCTCGGCGACGCCAAGAACCAGGTGGCCGC
CGCCGCCTCGCGCGTGGCCCTCAGCGCCGAGCGCCAGCCGCCGGTGGTCCAGGGCCTGGTGAAGAACGTGGTCAACTCCA
CCACCAGCAGCATGATGGGCAGCGTGCGCAACCAGCTCAACGCGGCCTGGATCAGCGACGTGGTCAGCGTCTACCGGCAG
TCCCTGGCCGGGCGCTACCCGATCGCCGCCGGCAGTTCGCGCGACGCCACCCTGGAGGACTTCGGCCACTTCTTCGGTGC
CGGCGGGGTGATGGACAGCTACTTCCGCCAGTACCTGCAACCCTACGTCGACACCTCCGCCAGCACCTGGCGCTGGCAGC
CCGGCGCGGCGCAGAAGCTCGGCATCAACCCCGGCGTGCTGCACACTTTCCAGCGCGCAGCGGCGATCCGCGACGCCTTC
TTCCGCTCCGGCGGCATGCAACCGACGGTGCGCTTCGAGCTCAAGCCGGTGACCATGGACGCGGCGATCAGCCAGTTCAT
CCTCGACCTCGACGGCCAGCAGCTGACCTACGACCACGGCCCCAGCCGCCCGGTGGCGATGCAGTGGCCGAGCGCCAACG
GCCTCGGCGTGGTCCGCCTGACGGTGACGCCGCCGCCCAGCAGCGGCCGTTCCGGCCTGACCCTGGAAGGCCCCTGGGCC
TGGTTCCGCCTGCTCGACCAGTCGGACCTGGAACGCGGCAACTCGCCGGACCGCTTCACCCTGCGGCTGCGCATCGACGG
CTCCAGCATCGCCTGCGAGCTGCGCGCCAGCAGCGCCTTCAACCCCTTCAAGAGCCGGGTCGTGTCCGGCTTCAGCCTCC
CGGAGCGCCTTTGA