Detailed information of component protein


Summary

Component ID T6CP015344
Component protein type TssI
T6SS ID (Type) T6SS01212 experimental (Type i4a)
Strain Pseudomonas aeruginosa PAK
Replicon chromosome
Sequence Protein sequence (668 a.a.); Nucleotide sequence (2004 bp)
Reference
[1] Chen R, Weng Y, Zhu F, et al. Polynucleotide Phosphorylase Regulates Multiple Virulence Factors and the Stabilities of Small RNAs RsmY/Z in Pseudomonas aeruginosa. Front Microbiol. 2016 Mar 2;7:247. doi: 10.3389/fmicb.2016.00247. eCollection 2016. PMID: 26973625
[2] Li K, Xu C, Jin Y, et al. SuhB is a regulator of multiple virulence genes and essential for pathogenesis of Pseudomonas aeruginosa. mBio. 2013 Oct 29;4(6):e00419-13. doi: 10.1128/mBio.00419-13. PMID: 24169572
[3] Brencic A, Lory S. Determination of the regulon and identification of novel mRNA targets of Pseudomonas aeruginosa RsmA. Mol Microbiol. 2009 May;72(3):612-32. doi: 10.1111/j.1365-2958.2009.06670.x. PMID: 19426209
[4] Marden JN, Diaz MR, Walton WG, et al. An unusual CsrA family member operates in series with RsmA to amplify posttranscriptional responses in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2013 Sep 10;110(37):15055-60. doi: 10.1073/pnas.1307217110. Epub 2013 Aug 26. PMID: 23980177
[5] Aragon IM, Pérez-Mendoza D, Moscoso JA, et al. Diguanylate cyclase DgcP is involved in plant and human Pseudomonas spp. infections. Environ Microbiol. 2015 Nov;17(11):4332-51. doi: 10.1111/1462-2920.12856. Epub 2015 Apr 28. PMID: 25809128
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) Y880_RS09215
Coordinate (Strand) 1930790..1932793 (+)
NCBI ID WP_016253420.1
RefSeq NZ_CP020659
Uniprot ID UPI000339ED7C
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 1.3e-80 29..321
Phage_base_V PF04717.14 1.5e-16 366..434
Gp5_C PF06715.14 1.3e-06 559..582
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-667 MSRGTDTSSLSLVAELLSDLNLETLAGGERLNTLDSMSLQGNSSTSLTLSNTVASHLSATLHNDSQQRPLDALVAEIHQLPADATADRFQLVLRPWLWWLTLASNNRVFQNLATSDIVTTIFKAHGFTDFKLSLTGSYEPREYCVQYGETDFAFVSRLLEEEGIFWFFIHEVGKHTLVLADSNDAFPPIPNGPKVPYLDQEIGVRELHGIRSAQYCLQAVAGTYSATDYEFTTPSTSLYSQAQAEAGPRARYEHPGGYTAKGRGDALTKQRVDGLRSQETRLIGESDCRWLVPGHWFTLSGHDDDSLNIDWVLTSVAHDASHAHYRNRFEAIPKATAYRPARVTPKPRMHTQTALVVGKAGEEIWTDQYGRIKIQFPWDRDGKNDETSSCWVRVVLPWSGKGFGMQFVPRIGQEVIVTFIDGDPDRPLVTGCVYNGDNALPYALPDNQTQSGIKTNSSKGGGGFNELRFEDKKDAEEVFLQAQKDLNVNVLNDSTASIGHDETLTVQNARTRTVKEGDETVTLEKGKRTVTIQTGSDSLDVKDTRTVTVGADQTHSTGGNYSHKVSGNFELTVDGNLTIKVSGTLALQSGGSLTLKSDADLAAQAGTSLTSKAGTSLTNQAGTSLTNKAGTSLTNDAGVSLTNKAGAEQTVDGGGMLTIKGGLVKVN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9432 - -


  Protein sequence: 668 a.a.    

>T6CP015344 NZ_CP020659:1930790-1932793 [Pseudomonas aeruginosa PAK] [TssI]
MSRGTDTSSLSLVAELLSDLNLETLAGGERLNTLDSMSLQGNSSTSLTLSNTVASHLSATLHNDSQQRPLDALVAEIHQL
PADATADRFQLVLRPWLWWLTLASNNRVFQNLATSDIVTTIFKAHGFTDFKLSLTGSYEPREYCVQYGETDFAFVSRLLE
EEGIFWFFIHEVGKHTLVLADSNDAFPPIPNGPKVPYLDQEIGVRELHGIRSAQYCLQAVAGTYSATDYEFTTPSTSLYS
QAQAEAGPRARYEHPGGYTAKGRGDALTKQRVDGLRSQETRLIGESDCRWLVPGHWFTLSGHDDDSLNIDWVLTSVAHDA
SHAHYRNRFEAIPKATAYRPARVTPKPRMHTQTALVVGKAGEEIWTDQYGRIKIQFPWDRDGKNDETSSCWVRVVLPWSG
KGFGMQFVPRIGQEVIVTFIDGDPDRPLVTGCVYNGDNALPYALPDNQTQSGIKTNSSKGGGGFNELRFEDKKDAEEVFL
QAQKDLNVNVLNDSTASIGHDETLTVQNARTRTVKEGDETVTLEKGKRTVTIQTGSDSLDVKDTRTVTVGADQTHSTGGN
YSHKVSGNFELTVDGNLTIKVSGTLALQSGGSLTLKSDADLAAQAGTSLTSKAGTSLTNQAGTSLTNKAGTSLTNDAGVS
LTNKAGAEQTVDGGGMLTIKGGLVKVN*

  Nucleotide sequence: 2004 bp    

>T6CP015344 NZ_CP020659:1930790-1932793 [Pseudomonas aeruginosa PAK] [TssI]
ATGTCTCGCGGCACTGATACCAGCAGCCTCTCATTAGTCGCCGAGTTGTTGAGCGACCTAAATCTGGAAACCCTAGCCGG
AGGCGAGAGGCTGAATACATTGGACAGCATGTCTCTCCAGGGCAACAGCTCCACGTCGCTGACACTATCCAACACCGTCG
CCTCGCACCTCAGCGCCACCCTGCACAACGACTCCCAGCAACGCCCCCTCGACGCCCTGGTCGCCGAAATCCACCAGCTC
CCCGCCGACGCAACTGCCGACCGTTTTCAACTCGTACTCAGACCATGGCTCTGGTGGCTGACCCTGGCCAGCAACAACCG
CGTGTTCCAGAACCTCGCCACCAGCGACATCGTCACCACGATCTTCAAGGCCCACGGCTTCACCGACTTCAAGCTGTCGC
TGACCGGCAGCTACGAGCCCCGTGAATACTGCGTGCAGTACGGCGAGACCGACTTCGCCTTCGTCTCGCGCCTGCTGGAG
GAGGAAGGCATCTTCTGGTTCTTCATCCACGAAGTGGGCAAGCACACGTTGGTGCTGGCGGACAGCAACGACGCCTTCCC
GCCGATCCCCAACGGGCCCAAGGTGCCATACCTGGACCAGGAGATCGGCGTGCGCGAACTGCACGGCATCCGTTCGGCGC
AGTACTGCCTGCAGGCGGTCGCCGGGACCTACAGCGCCACCGACTACGAGTTCACCACACCCAGTACTTCGCTCTATTCC
CAGGCACAGGCCGAGGCCGGGCCGCGCGCACGGTACGAACACCCCGGCGGCTACACCGCCAAGGGCCGCGGCGATGCGCT
GACCAAGCAGCGCGTGGACGGCCTGCGCAGCCAGGAGACGCGCCTGATCGGCGAGAGCGACTGCCGCTGGCTGGTGCCCG
GCCACTGGTTCACCCTCAGCGGGCATGACGACGATTCGCTGAATATCGACTGGGTGCTGACCTCGGTCGCCCACGACGCC
AGCCACGCGCACTACCGCAACCGCTTCGAGGCGATCCCCAAGGCCACCGCCTACCGCCCCGCGCGGGTCACGCCCAAGCC
GCGCATGCACACCCAGACCGCCCTGGTGGTGGGCAAGGCCGGCGAGGAGATCTGGACCGACCAGTACGGCCGGATCAAGA
TCCAGTTCCCCTGGGACCGCGACGGCAAGAACGACGAGACCTCCTCCTGCTGGGTGCGCGTGGTGCTGCCCTGGAGCGGC
AAGGGCTTCGGCATGCAGTTCGTGCCGCGCATCGGCCAGGAAGTGATCGTCACCTTCATCGACGGCGACCCGGACCGCCC
GCTGGTCACCGGCTGCGTCTACAACGGCGACAACGCCCTGCCCTACGCGCTGCCGGACAACCAGACGCAGTCCGGGATCA
AGACCAACTCGTCGAAGGGCGGCGGCGGTTTCAACGAGCTGCGCTTCGAGGACAAGAAGGACGCCGAGGAAGTCTTCCTC
CAGGCGCAGAAGGACCTCAACGTCAACGTGCTCAACGACAGCACCGCCAGCATCGGCCACGACGAGACCCTGACGGTGCA
GAACGCCCGCACCCGCACGGTGAAGGAAGGCGACGAGACCGTCACCCTGGAGAAAGGCAAGCGCACGGTGACCATCCAGA
CCGGCAGCGACAGTCTGGATGTGAAGGACACGCGCACGGTGACGGTGGGCGCCGACCAGACCCACAGCACCGGCGGCAAC
TACAGCCACAAGGTCAGCGGCAACTTCGAGCTGACGGTGGACGGCAACCTGACCATCAAGGTCAGCGGCACCCTCGCCCT
GCAGAGCGGCGGCAGCCTGACCCTGAAGAGCGACGCCGACCTCGCCGCCCAGGCTGGCACCTCGCTGACCAGCAAGGCCG
GGACGTCGCTGACCAACCAGGCCGGCACCTCCCTCACCAACAAGGCCGGCACCAGCCTGACCAACGATGCCGGCGTCAGC
CTGACCAACAAGGCCGGCGCCGAACAGACGGTGGACGGCGGCGGCATGCTGACCATCAAGGGCGGCCTGGTAAAGGTCAA
CTGA