Detailed information of component protein


Summary

Component ID T6CP015343
Component protein type TssH
T6SS ID (Type) T6SS01212 experimental (Type i4a)
Strain Pseudomonas aeruginosa PAK
Replicon chromosome
Sequence Protein sequence (850 a.a.); Nucleotide sequence (2550 bp)
Reference
[1] Chen R, Weng Y, Zhu F, et al. Polynucleotide Phosphorylase Regulates Multiple Virulence Factors and the Stabilities of Small RNAs RsmY/Z in Pseudomonas aeruginosa. Front Microbiol. 2016 Mar 2;7:247. doi: 10.3389/fmicb.2016.00247. eCollection 2016. PMID: 26973625
[2] Li K, Xu C, Jin Y, et al. SuhB is a regulator of multiple virulence genes and essential for pathogenesis of Pseudomonas aeruginosa. mBio. 2013 Oct 29;4(6):e00419-13. doi: 10.1128/mBio.00419-13. PMID: 24169572
[3] Brencic A, Lory S. Determination of the regulon and identification of novel mRNA targets of Pseudomonas aeruginosa RsmA. Mol Microbiol. 2009 May;72(3):612-32. doi: 10.1111/j.1365-2958.2009.06670.x. PMID: 19426209
[4] Marden JN, Diaz MR, Walton WG, et al. An unusual CsrA family member operates in series with RsmA to amplify posttranscriptional responses in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2013 Sep 10;110(37):15055-60. doi: 10.1073/pnas.1307217110. Epub 2013 Aug 26. PMID: 23980177
[5] Aragon IM, Pérez-Mendoza D, Moscoso JA, et al. Diguanylate cyclase DgcP is involved in plant and human Pseudomonas spp. infections. Environ Microbiol. 2015 Nov;17(11):4332-51. doi: 10.1111/1462-2920.12856. Epub 2015 Apr 28. PMID: 25809128
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) Y880_RS09200
Coordinate (Strand) 1926413..1928962 (+)
NCBI ID WP_016253421.1
RefSeq NZ_CP020659
Uniprot ID UPI000340FB60
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 3.1e-36 611..775
AAA_lid_9 PF17871.3 2.8e-29 360..453
Clp_N PF02861.22 2.5e-10 21..71
AAA PF00004.31 3.2e-10 220..332
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-849 MELAALIGRLNPDCRRALERAAQRCLQRTHHYVEIEHLLLELLDIDGGDFACLLPRFGLERDALVAEINLSLELFKAGNTRTPALSAHTIGLLEDAVVHASVLGQAQIRSGLLLLALLDREERRALLLNSASSLLRIPHEALQANLLEWIQASREQPPAPNRPAAGGDKPESAPDPLLDQYTQDLTAEARAGRIDPIVGRDGEIRQCVDILLRRRQNNPILVGAPGVGKTAVVEGLALRIAAGEVPPSLQEVILRVLDLGLLQAGASMKGEFEQRLKGVIDAVRNSAQPIILFIDEAHTLIGAGGAEGGSDAANLLKPALARGELRTLAATTWLEYKKYFEKDPALTRRFQLVQVEEPDEATAVEMLRGVAGKLELHHGVQIMDAAIVDAVKLSHRYISGRQLPDKAISVLDTACARVALGQHDVPPPLESLRHREQALEEELQRLRREQATGLDHSARITALESESGDNRRTIRELETRWDEEREAVRELLDTRRELLALSESADAAKPDEELDGRIDHLAAELARLAAGLEAIRQDDPLVPEQVDSRTVAAVIAGWTGIPVGKMLADEAHAIRSLAQRMGQRVMGQEAALGAIAQRIQAYRAGLSDPAKPVGVFLLPGPTGVGKTETAYALADALYGGERNLISINLSEYQEAHTVSQLKGAPPGYVGYGSGGVLTEAVRRKPYSVVLLDEIEKAHPDVLEAFYNVFDKGVMEDGTGLVVDFRNTVILATSNVGAELLLDSPAEQVATPAFDERLRKVLLQTFRPAFLARMTVVPYRPLEEATLEGIVVAKLEKLRERYKAATGKQFDFDPAIVKAVLAKCSSAGARDIENVLMAEVTGKLAEWVLE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9977 - -


  Protein sequence: 850 a.a.    

>T6CP015343 NZ_CP020659:1926413-1928962 [Pseudomonas aeruginosa PAK] [TssH]
MELAALIGRLNPDCRRALERAAQRCLQRTHHYVEIEHLLLELLDIDGGDFACLLPRFGLERDALVAEINLSLELFKAGNT
RTPALSAHTIGLLEDAVVHASVLGQAQIRSGLLLLALLDREERRALLLNSASSLLRIPHEALQANLLEWIQASREQPPAP
NRPAAGGDKPESAPDPLLDQYTQDLTAEARAGRIDPIVGRDGEIRQCVDILLRRRQNNPILVGAPGVGKTAVVEGLALRI
AAGEVPPSLQEVILRVLDLGLLQAGASMKGEFEQRLKGVIDAVRNSAQPIILFIDEAHTLIGAGGAEGGSDAANLLKPAL
ARGELRTLAATTWLEYKKYFEKDPALTRRFQLVQVEEPDEATAVEMLRGVAGKLELHHGVQIMDAAIVDAVKLSHRYISG
RQLPDKAISVLDTACARVALGQHDVPPPLESLRHREQALEEELQRLRREQATGLDHSARITALESESGDNRRTIRELETR
WDEEREAVRELLDTRRELLALSESADAAKPDEELDGRIDHLAAELARLAAGLEAIRQDDPLVPEQVDSRTVAAVIAGWTG
IPVGKMLADEAHAIRSLAQRMGQRVMGQEAALGAIAQRIQAYRAGLSDPAKPVGVFLLPGPTGVGKTETAYALADALYGG
ERNLISINLSEYQEAHTVSQLKGAPPGYVGYGSGGVLTEAVRRKPYSVVLLDEIEKAHPDVLEAFYNVFDKGVMEDGTGL
VVDFRNTVILATSNVGAELLLDSPAEQVATPAFDERLRKVLLQTFRPAFLARMTVVPYRPLEEATLEGIVVAKLEKLRER
YKAATGKQFDFDPAIVKAVLAKCSSAGARDIENVLMAEVTGKLAEWVLE*

  Nucleotide sequence: 2550 bp    

>T6CP015343 NZ_CP020659:1926413-1928962 [Pseudomonas aeruginosa PAK] [TssH]
ATGGAACTCGCCGCCCTGATCGGCCGCCTCAACCCGGATTGTCGCCGCGCCCTGGAGCGCGCCGCGCAACGCTGCCTGCA
ACGCACCCATCATTACGTAGAGATCGAGCACCTGCTGCTGGAGCTGCTGGACATCGACGGCGGCGACTTCGCCTGCCTGC
TGCCGCGCTTCGGCCTGGAGCGCGACGCCCTGGTCGCCGAGATCAACCTGTCGCTGGAGCTGTTCAAGGCCGGCAATACC
CGCACTCCGGCGCTGTCCGCGCACACCATCGGCCTGCTCGAAGACGCCGTGGTGCACGCCAGTGTGCTCGGCCAGGCGCA
GATCCGTTCCGGCCTGCTGCTGCTCGCCTTGCTCGACCGCGAGGAGCGCCGCGCCCTGTTGCTGAACAGCGCGTCGTCGC
TGCTGCGGATTCCCCACGAGGCCTTGCAGGCCAACCTGCTGGAGTGGATCCAGGCCTCCCGCGAACAGCCGCCCGCGCCG
AACCGCCCGGCGGCAGGCGGCGACAAGCCGGAAAGCGCCCCGGACCCGCTGCTCGACCAGTACACCCAGGACCTCACCGC
CGAGGCCCGCGCCGGGCGCATCGACCCCATAGTCGGGCGCGACGGGGAGATCCGCCAGTGCGTCGACATCCTCCTGCGCC
GGCGGCAGAACAACCCGATCCTGGTCGGCGCGCCGGGCGTCGGCAAGACCGCTGTGGTCGAGGGCCTGGCCCTGCGCATC
GCCGCCGGCGAGGTGCCGCCGTCGTTGCAGGAGGTGATCCTGCGGGTGCTCGACCTCGGCCTGTTGCAGGCCGGCGCCAG
CATGAAGGGCGAGTTCGAGCAGCGCCTCAAGGGCGTGATCGACGCCGTGCGCAACAGCGCGCAGCCGATCATCCTGTTCA
TCGACGAGGCGCACACGCTGATCGGCGCCGGCGGCGCGGAAGGCGGCAGCGACGCCGCCAACCTGCTCAAGCCGGCCCTG
GCGCGCGGCGAGTTGCGCACCCTGGCGGCCACCACCTGGCTGGAATACAAGAAATACTTCGAGAAGGACCCGGCGCTGAC
CCGGCGCTTCCAGCTGGTCCAGGTCGAGGAGCCGGACGAGGCCACCGCCGTGGAGATGCTGCGCGGCGTCGCCGGCAAGC
TGGAACTGCATCACGGCGTGCAGATCATGGACGCGGCCATCGTCGATGCGGTGAAGCTGTCGCATCGCTACATCTCCGGC
CGCCAGTTGCCGGACAAGGCGATCAGCGTGCTCGACACCGCCTGCGCGCGGGTCGCCCTCGGCCAGCACGACGTGCCGCC
GCCGCTGGAAAGCCTGCGCCATCGCGAGCAGGCGCTGGAAGAGGAATTGCAGCGGCTGCGCCGGGAACAGGCCACCGGCC
TCGACCACAGCGCGCGTATCACCGCCCTGGAAAGCGAGTCGGGCGATAACCGCCGGACCATCCGCGAGCTGGAGACCCGC
TGGGACGAGGAACGCGAAGCGGTGCGCGAACTGCTCGACACCCGCCGCGAACTGCTGGCCCTCAGCGAAAGCGCTGACGC
GGCCAAGCCCGACGAGGAACTGGACGGTCGCATCGACCACCTGGCCGCCGAACTGGCGCGCCTGGCGGCCGGCCTCGAAG
CCATCCGCCAGGACGACCCGCTGGTTCCCGAGCAGGTGGACTCGCGTACCGTGGCCGCGGTGATCGCCGGCTGGACCGGC
ATCCCGGTGGGCAAGATGCTCGCCGACGAAGCCCACGCCATCCGTTCCCTGGCGCAACGAATGGGCCAGCGGGTGATGGG
CCAGGAGGCCGCCCTGGGCGCCATCGCCCAGCGCATCCAGGCCTATCGCGCCGGACTCAGCGACCCGGCCAAGCCGGTCG
GCGTATTCCTCCTGCCCGGCCCCACCGGCGTGGGCAAGACCGAGACCGCCTACGCCCTGGCCGACGCCCTCTACGGCGGC
GAACGCAACCTGATCAGCATCAACCTCTCCGAGTACCAGGAGGCCCACACCGTCAGCCAGCTCAAGGGCGCCCCGCCCGG
CTACGTCGGCTACGGCAGCGGCGGCGTGCTCACCGAAGCGGTGCGCCGCAAGCCCTATTCGGTGGTGCTGCTGGACGAGA
TCGAGAAAGCCCATCCGGACGTGCTGGAAGCCTTCTACAACGTGTTCGACAAGGGCGTGATGGAAGACGGCACCGGCCTG
GTGGTGGACTTCAGGAACACCGTGATCCTCGCCACCAGCAACGTCGGCGCCGAACTGCTGCTGGACAGCCCGGCCGAACA
GGTCGCCACCCCGGCCTTCGACGAGCGCCTGCGCAAAGTCCTGCTGCAAACCTTCCGCCCGGCGTTCCTCGCGCGCATGA
CCGTGGTGCCTTACCGGCCGCTGGAGGAAGCCACCCTGGAAGGCATCGTCGTGGCCAAGCTGGAAAAACTGCGGGAACGC
TACAAGGCCGCTACCGGCAAACAGTTCGACTTCGACCCGGCCATCGTCAAGGCAGTACTCGCCAAGTGCAGTTCGGCGGG
CGCGCGGGATATCGAGAATGTGTTGATGGCGGAGGTGACGGGGAAGTTGGCGGAGTGGGTGTTGGAGTAG