Detailed information of component protein


Summary

Component ID T6CP015339
Component protein type TssC
T6SS ID (Type) T6SS01212 experimental (Type i4a)
Strain Pseudomonas aeruginosa PAK
Replicon chromosome
Sequence Protein sequence (495 a.a.); Nucleotide sequence (1485 bp)
Reference
[1] Chen R, Weng Y, Zhu F, et al. Polynucleotide Phosphorylase Regulates Multiple Virulence Factors and the Stabilities of Small RNAs RsmY/Z in Pseudomonas aeruginosa. Front Microbiol. 2016 Mar 2;7:247. doi: 10.3389/fmicb.2016.00247. eCollection 2016. PMID: 26973625
[2] Li K, Xu C, Jin Y, et al. SuhB is a regulator of multiple virulence genes and essential for pathogenesis of Pseudomonas aeruginosa. mBio. 2013 Oct 29;4(6):e00419-13. doi: 10.1128/mBio.00419-13. PMID: 24169572
[3] Brencic A, Lory S. Determination of the regulon and identification of novel mRNA targets of Pseudomonas aeruginosa RsmA. Mol Microbiol. 2009 May;72(3):612-32. doi: 10.1111/j.1365-2958.2009.06670.x. PMID: 19426209
[4] Marden JN, Diaz MR, Walton WG, et al. An unusual CsrA family member operates in series with RsmA to amplify posttranscriptional responses in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2013 Sep 10;110(37):15055-60. doi: 10.1073/pnas.1307217110. Epub 2013 Aug 26. PMID: 23980177
[5] Aragon IM, Pérez-Mendoza D, Moscoso JA, et al. Diguanylate cyclase DgcP is involved in plant and human Pseudomonas spp. infections. Environ Microbiol. 2015 Nov;17(11):4332-51. doi: 10.1111/1462-2920.12856. Epub 2015 Apr 28. PMID: 25809128
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) Y880_RS09175
Coordinate (Strand) 1921292..1922776 (+)
NCBI ID WP_016253422.1
RefSeq NZ_CP020659
Uniprot ID UPI00033FD691
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
VipB PF05943.14 1.8e-137 68..368
VipB_2 PF18945.2 8.4e-46 377..488
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-494 MPKSSAAEQSGESSTQTLSLLDEIIAKGRMAHDDSQQDYARDMLAEFATQVLDEGMAVDKDTVAMINDRISQIDALISDQLNQIIHHPELQKLEASWRGLHQLVSNTETSARLKLRLLNVGKNELQNDLEKAVEFDQSALFKKIYEEEYGTFGGHPFSLLIGDFTFGRHPQDIGLLEKLSNVAAAAHAPFIAAASPRLFDMNSFTELAVPRDLTKIFESLELIKWRAFRESEDSRYVSLVLPNFLLRLPYGPETRPVEGMNYVEDVNGSDHSKYLWGNAAWVLAQRITEAFAKYGWCAAIRGAEGGGAVEGLPAHTFRTSSGDLSLKCPTEVAITDRREKELNDLGFISLCHKKNSDVAVFFGGQTTNKARLYNTNEANANARLSAMLPYVLAASRFAHYLKVIMRDKVGSFMTRDNVQTYLNNWIADYVLINDNAPQEIKAQYPLREARVDVSEVAGKPGAYRATVFLRPHFQLEELSASIRLVANLPPPVAA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9845 - -


  Protein sequence: 495 a.a.    

>T6CP015339 NZ_CP020659:1921292-1922776 [Pseudomonas aeruginosa PAK] [TssC]
MPKSSAAEQSGESSTQTLSLLDEIIAKGRMAHDDSQQDYARDMLAEFATQVLDEGMAVDKDTVAMINDRISQIDALISDQ
LNQIIHHPELQKLEASWRGLHQLVSNTETSARLKLRLLNVGKNELQNDLEKAVEFDQSALFKKIYEEEYGTFGGHPFSLL
IGDFTFGRHPQDIGLLEKLSNVAAAAHAPFIAAASPRLFDMNSFTELAVPRDLTKIFESLELIKWRAFRESEDSRYVSLV
LPNFLLRLPYGPETRPVEGMNYVEDVNGSDHSKYLWGNAAWVLAQRITEAFAKYGWCAAIRGAEGGGAVEGLPAHTFRTS
SGDLSLKCPTEVAITDRREKELNDLGFISLCHKKNSDVAVFFGGQTTNKARLYNTNEANANARLSAMLPYVLAASRFAHY
LKVIMRDKVGSFMTRDNVQTYLNNWIADYVLINDNAPQEIKAQYPLREARVDVSEVAGKPGAYRATVFLRPHFQLEELSA
SIRLVANLPPPVAA*

  Nucleotide sequence: 1485 bp    

>T6CP015339 NZ_CP020659:1921292-1922776 [Pseudomonas aeruginosa PAK] [TssC]
ATGCCCAAGTCATCCGCCGCCGAACAATCCGGCGAGAGTTCGACCCAGACCCTGTCCCTGCTCGACGAGATCATCGCCAA
GGGCCGCATGGCCCACGACGACAGCCAGCAGGACTACGCCCGCGACATGCTCGCGGAATTCGCCACCCAGGTCCTCGACG
AGGGCATGGCCGTCGACAAGGACACCGTGGCGATGATCAACGACCGCATCAGCCAAATCGATGCGCTGATCAGCGACCAG
CTCAACCAGATCATCCACCACCCCGAGTTGCAGAAGCTGGAAGCCTCCTGGCGTGGCCTGCACCAACTGGTGAGCAACAC
CGAGACCAGCGCGCGGCTCAAGCTGCGCCTGCTGAACGTCGGCAAGAACGAACTGCAGAACGACCTGGAGAAGGCGGTCG
AATTCGACCAGAGCGCACTGTTCAAGAAGATCTACGAAGAGGAATACGGCACCTTCGGCGGACACCCCTTCAGCCTGCTG
ATCGGCGACTTCACCTTCGGTCGCCATCCGCAGGACATCGGCCTGCTGGAGAAGCTGTCGAACGTCGCCGCGGCTGCCCA
CGCGCCGTTCATCGCCGCCGCCAGCCCACGCCTGTTCGACATGAACAGCTTCACCGAACTGGCCGTGCCACGCGACCTGA
CCAAGATCTTCGAGAGCCTGGAGCTGATCAAGTGGCGCGCCTTCCGCGAGAGCGAGGACTCGCGCTACGTGTCGCTGGTG
CTGCCGAACTTCCTCTTGCGCCTGCCCTACGGCCCGGAGACGCGACCGGTGGAAGGCATGAACTATGTCGAGGACGTCAA
CGGCTCCGACCACTCCAAGTACCTCTGGGGCAACGCCGCCTGGGTCCTGGCGCAGCGCATCACCGAGGCCTTCGCCAAGT
ACGGCTGGTGCGCGGCGATCCGCGGCGCGGAAGGCGGCGGCGCGGTCGAGGGCCTACCGGCGCACACGTTCCGCACCAGC
TCCGGCGACCTGTCGCTGAAGTGCCCGACCGAGGTGGCGATCACCGACCGCCGCGAGAAGGAACTCAACGATCTCGGCTT
CATTTCCCTGTGCCACAAGAAGAACAGCGACGTGGCGGTGTTCTTCGGCGGCCAGACCACCAACAAGGCCAGGCTCTACA
ACACCAACGAGGCCAACGCCAACGCGCGCCTGTCGGCGATGCTGCCGTACGTGCTGGCGGCATCGCGCTTCGCCCACTAC
CTGAAGGTGATCATGCGCGACAAGGTCGGCAGCTTCATGACCCGCGACAACGTGCAGACCTACCTGAACAACTGGATCGC
CGACTACGTGCTGATCAACGACAACGCACCGCAGGAAATCAAGGCGCAGTACCCGCTGCGCGAGGCGCGGGTAGATGTCA
GCGAGGTGGCCGGCAAACCGGGGGCCTACCGCGCCACGGTGTTCCTCCGGCCGCACTTCCAGCTCGAGGAACTCAGCGCG
TCGATCCGCCTGGTCGCCAACCTGCCGCCGCCGGTAGCGGCGTGA