Detailed information of component protein


Summary

Component ID T6CP015335
Component protein type TssM
T6SS ID (Type) T6SS01212 experimental (Type i4a)
Strain Pseudomonas aeruginosa PAK
Replicon chromosome
Sequence Protein sequence (1272 a.a.); Nucleotide sequence (3816 bp)
Reference
[1] Chen R, Weng Y, Zhu F, et al. Polynucleotide Phosphorylase Regulates Multiple Virulence Factors and the Stabilities of Small RNAs RsmY/Z in Pseudomonas aeruginosa. Front Microbiol. 2016 Mar 2;7:247. doi: 10.3389/fmicb.2016.00247. eCollection 2016. PMID: 26973625
[2] Li K, Xu C, Jin Y, et al. SuhB is a regulator of multiple virulence genes and essential for pathogenesis of Pseudomonas aeruginosa. mBio. 2013 Oct 29;4(6):e00419-13. doi: 10.1128/mBio.00419-13. PMID: 24169572
[3] Brencic A, Lory S. Determination of the regulon and identification of novel mRNA targets of Pseudomonas aeruginosa RsmA. Mol Microbiol. 2009 May;72(3):612-32. doi: 10.1111/j.1365-2958.2009.06670.x. PMID: 19426209
[4] Marden JN, Diaz MR, Walton WG, et al. An unusual CsrA family member operates in series with RsmA to amplify posttranscriptional responses in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2013 Sep 10;110(37):15055-60. doi: 10.1073/pnas.1307217110. Epub 2013 Aug 26. PMID: 23980177
[5] Aragon IM, Pérez-Mendoza D, Moscoso JA, et al. Diguanylate cyclase DgcP is involved in plant and human Pseudomonas spp. infections. Environ Microbiol. 2015 Nov;17(11):4332-51. doi: 10.1111/1462-2920.12856. Epub 2015 Apr 28. PMID: 25809128
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) Y880_RS09150
Coordinate (Strand) 1914861..1918676 (-)
NCBI ID WP_016253424.1
RefSeq NZ_CP020659
Uniprot ID UPI00033BA5BE
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 6.9e-48 115..352
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-4 MSGA
Transmembrane helix 5-27 TLFMLVLLLVLLALLLGALGWWW
Inside 28-348 RTRGGTEIRSFYAAVRQMEREQGWQGRYEAPWLLMLGNETEGEQLCATWRLLPVARPAWFGRWWSDGEGAILLVPESVFLPDEGLRRQSGAWLRLLRLFLRLRGRRALDGVVWNIPLARLQDGEQAANLGLAARRRYVELTQRLGLSLPVYVVITGLEDLPGFQELLAVLPEEARERALGWSSPFAAEAAWQSRWCEQALEEITATLTESIVELGTLRGQVDNELYCLPPRLESLRGSLQALLEPVFQGNARGEAPRFRGLYLSGSEAAGAAADEVLPAVDAPRRRSSFASQLWARRILAEEGLAQAVPRILQLRQRWQRG
Transmembrane helix 349-368 IGLAALCLCLLWGGLMTWVW
Outside 369-1271 RDALRDAGELSQLLHGASERYQPLDDDTRRAAQVRQNVQAWWQLVSQAPRWRFTSLAFPSSWFSSLDARIDNAYRRVSERLLVRPLRSLLEGEASDLRAIRSDGQPGLKEGDDPSQWKDYLAAKDLVARATTLERHNRLFAQAIDNRRTPLDDLLQLSNDALGSSYNAGSLARLAYYNRTLFAERPADLAALDLGRVAATAADNFHDLMARWLNAYFLTDSLERTGNALTQELAQLGQERDATAAQLLGIGELINHLQQQVNRINLIWGNGVGQDLVPGYQNLLKSAQQSSLLGNRVEELQNLTVQLQQQFRSEWIAPPEAAGDGLLVKQGATLKLADDLLGLKRAIDDLKTKDFVSVALADKTLADHSLLSIDDIGVTQALSFFNDFTGYYESTLPSLNPRYRYSVLHAAASAATEAMWQSLGPRSRSLASRNASRFDVQVKQVQVLQAALLELQDLQAGARLLLSLNALAVADIGQALRDIDEQAVLREPVDFSRWSGAPNFGLQMFRAQDSAELKQSLNSQFNAMAAVAERHAPALEWLQAQQSSLATADYNAFVRFSALNAELQKFKADNPASTAAQLAKLVGNDFNQMDIGSCADILNGVLLPSSRSDLATRLVDLRQGALGRCQSLQQQQAAQAWKDLADYFNQYLAGRFPFAYSLEAADAEPGRVRHLLKLMETRLPQVREGLAQVRSPDLPAAEDFVRRLEQAQRWLGPLFERDKSGLLGVALDIDWRSDRSLERGADQVIAWSLYSGDQESRFPGAQDKGLTWSVGDPLKIMLRWAKNGSQRPADDPRQASLAVADLEAGWSYQGSWALLRMMRAHFSRQRPPNVDYTEFPLVLQLPVYAPYSPENEARMFLRLSLMSLGGKTPLSIQPLPVRAPQSPFATLLPATVASTGGTP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9930 - -


  Protein sequence: 1272 a.a.    

>T6CP015335 NZ_CP020659:c1918676-1914861 [Pseudomonas aeruginosa PAK] [TssM]
MSGATLFMLVLLLVLLALLLGALGWWWRTRGGTEIRSFYAAVRQMEREQGWQGRYEAPWLLMLGNETEGEQLCATWRLLP
VARPAWFGRWWSDGEGAILLVPESVFLPDEGLRRQSGAWLRLLRLFLRLRGRRALDGVVWNIPLARLQDGEQAANLGLAA
RRRYVELTQRLGLSLPVYVVITGLEDLPGFQELLAVLPEEARERALGWSSPFAAEAAWQSRWCEQALEEITATLTESIVE
LGTLRGQVDNELYCLPPRLESLRGSLQALLEPVFQGNARGEAPRFRGLYLSGSEAAGAAADEVLPAVDAPRRRSSFASQL
WARRILAEEGLAQAVPRILQLRQRWQRGIGLAALCLCLLWGGLMTWVWRDALRDAGELSQLLHGASERYQPLDDDTRRAA
QVRQNVQAWWQLVSQAPRWRFTSLAFPSSWFSSLDARIDNAYRRVSERLLVRPLRSLLEGEASDLRAIRSDGQPGLKEGD
DPSQWKDYLAAKDLVARATTLERHNRLFAQAIDNRRTPLDDLLQLSNDALGSSYNAGSLARLAYYNRTLFAERPADLAAL
DLGRVAATAADNFHDLMARWLNAYFLTDSLERTGNALTQELAQLGQERDATAAQLLGIGELINHLQQQVNRINLIWGNGV
GQDLVPGYQNLLKSAQQSSLLGNRVEELQNLTVQLQQQFRSEWIAPPEAAGDGLLVKQGATLKLADDLLGLKRAIDDLKT
KDFVSVALADKTLADHSLLSIDDIGVTQALSFFNDFTGYYESTLPSLNPRYRYSVLHAAASAATEAMWQSLGPRSRSLAS
RNASRFDVQVKQVQVLQAALLELQDLQAGARLLLSLNALAVADIGQALRDIDEQAVLREPVDFSRWSGAPNFGLQMFRAQ
DSAELKQSLNSQFNAMAAVAERHAPALEWLQAQQSSLATADYNAFVRFSALNAELQKFKADNPASTAAQLAKLVGNDFNQ
MDIGSCADILNGVLLPSSRSDLATRLVDLRQGALGRCQSLQQQQAAQAWKDLADYFNQYLAGRFPFAYSLEAADAEPGRV
RHLLKLMETRLPQVREGLAQVRSPDLPAAEDFVRRLEQAQRWLGPLFERDKSGLLGVALDIDWRSDRSLERGADQVIAWS
LYSGDQESRFPGAQDKGLTWSVGDPLKIMLRWAKNGSQRPADDPRQASLAVADLEAGWSYQGSWALLRMMRAHFSRQRPP
NVDYTEFPLVLQLPVYAPYSPENEARMFLRLSLMSLGGKTPLSIQPLPVRAPQSPFATLLPATVASTGGTP*

  Nucleotide sequence: 3816 bp    

>T6CP015335 NZ_CP020659:c1918676-1914861 [Pseudomonas aeruginosa PAK] [TssM]
ATGAGCGGCGCGACCCTGTTCATGCTGGTTCTCCTCCTGGTGCTGCTGGCGCTGCTGCTGGGGGCGCTGGGCTGGTGGTG
GCGGACCCGGGGCGGTACGGAGATCCGCAGCTTCTACGCGGCGGTCCGGCAGATGGAGCGCGAGCAGGGCTGGCAGGGCC
GCTACGAAGCGCCCTGGCTGCTGATGCTCGGCAACGAGACCGAGGGCGAGCAGTTGTGCGCCACCTGGCGCCTGCTGCCG
GTGGCGCGTCCGGCCTGGTTCGGCCGCTGGTGGTCGGACGGCGAGGGGGCGATCCTGCTGGTGCCCGAGTCGGTGTTCCT
GCCCGACGAGGGCCTGCGCCGGCAAAGCGGCGCCTGGCTGCGCCTGTTGCGTCTGTTCCTGCGCCTGCGCGGCAGGCGCG
CGCTGGACGGGGTGGTCTGGAACATTCCGCTGGCGCGGTTGCAGGATGGCGAGCAGGCGGCCAACCTCGGCCTCGCCGCG
CGCCGTCGCTACGTGGAACTGACCCAGCGCCTTGGCCTGAGCCTGCCGGTTTACGTGGTGATCACTGGCCTGGAAGACCT
GCCGGGTTTCCAGGAACTTCTCGCGGTGCTGCCCGAGGAGGCGCGCGAGCGCGCCCTCGGCTGGTCCTCGCCGTTCGCCG
CCGAAGCGGCCTGGCAGTCGCGCTGGTGCGAGCAGGCGCTGGAGGAAATCACCGCCACCCTGACCGAGTCGATCGTCGAA
CTCGGTACCTTGCGCGGCCAGGTGGACAATGAGCTGTATTGCCTGCCGCCACGCCTGGAAAGCCTCCGCGGTTCGTTGCA
GGCGCTGCTCGAACCGGTCTTCCAGGGCAACGCCAGGGGCGAGGCGCCGCGCTTTCGCGGTCTCTACCTGAGCGGCAGCG
AGGCGGCGGGCGCGGCAGCCGACGAAGTCCTGCCGGCGGTCGACGCGCCGCGTCGGCGCAGCAGCTTCGCCAGCCAACTG
TGGGCACGGCGGATCCTTGCCGAGGAAGGCCTGGCCCAGGCGGTGCCGCGCATCCTCCAGCTGCGTCAGCGCTGGCAGCG
CGGGATCGGCCTCGCCGCGTTGTGCCTGTGCCTGCTCTGGGGCGGCCTGATGACCTGGGTCTGGCGCGACGCGCTGCGCG
ACGCCGGTGAACTGTCGCAATTGCTGCATGGCGCCAGTGAGCGCTACCAGCCGCTCGACGACGACACTCGGCGCGCCGCC
CAGGTCCGGCAGAACGTCCAGGCCTGGTGGCAACTGGTGTCACAGGCGCCGCGCTGGCGCTTCACTTCGCTGGCGTTTCC
CAGTTCCTGGTTTTCCTCCCTGGATGCACGTATCGACAACGCGTATCGCCGCGTTTCCGAGCGCCTGCTGGTGCGACCGC
TGCGCAGCCTGCTGGAAGGCGAGGCGAGCGACCTGCGGGCGATCCGCAGCGATGGCCAACCCGGCCTGAAGGAGGGCGAC
GACCCCAGCCAGTGGAAGGACTACCTGGCGGCGAAGGACCTGGTCGCCCGCGCCACTACGCTGGAGCGCCACAATCGCTT
GTTCGCCCAGGCCATCGACAACCGCAGGACGCCGCTCGACGACCTTCTGCAACTGAGTAACGATGCCTTGGGCAGCAGCT
ACAACGCCGGCAGCCTGGCGCGCCTGGCCTATTACAACCGCACGCTGTTCGCCGAGCGTCCGGCCGATCTCGCCGCCCTC
GACCTGGGCCGGGTCGCCGCCACGGCGGCGGATAACTTCCATGACCTGATGGCACGCTGGCTGAACGCCTACTTCCTCAC
CGACAGCCTGGAACGCACCGGCAATGCCCTGACCCAGGAACTCGCGCAGCTCGGCCAGGAACGAGACGCCACGGCGGCCC
AGTTGCTGGGGATCGGCGAATTGATCAACCACCTGCAGCAACAGGTCAACCGCATCAACCTGATCTGGGGCAATGGCGTC
GGCCAGGATCTCGTCCCTGGCTACCAGAACCTGCTCAAGAGCGCCCAGCAAAGCAGCTTGCTGGGAAACCGCGTGGAGGA
ACTGCAGAACCTCACTGTGCAGTTGCAGCAGCAGTTTCGCAGCGAATGGATCGCGCCTCCGGAGGCTGCCGGCGACGGCC
TGCTGGTGAAACAGGGGGCGACGCTCAAGCTGGCCGATGACCTGCTCGGTCTGAAACGCGCCATCGATGACCTGAAGACC
AAGGACTTCGTCTCCGTGGCCCTGGCGGACAAGACACTGGCGGACCACTCCTTGCTGAGCATCGACGATATCGGCGTGAC
TCAGGCGTTGAGCTTCTTCAACGACTTCACCGGCTACTACGAGAGCACCCTGCCATCGCTCAACCCGCGCTATCGCTATT
CGGTCCTGCACGCCGCCGCCAGCGCGGCGACCGAGGCCATGTGGCAAAGCCTCGGGCCGCGCAGTCGTAGCCTGGCCAGT
CGCAATGCCTCGCGTTTCGACGTGCAGGTCAAGCAGGTCCAGGTGTTGCAGGCGGCGCTGCTGGAGTTGCAGGACCTGCA
GGCCGGCGCGCGCCTGCTGCTCAGCCTCAACGCGCTGGCCGTGGCCGATATCGGCCAGGCCCTGCGCGACATCGACGAGC
AGGCGGTGCTGCGCGAGCCGGTCGATTTCAGCCGCTGGTCGGGCGCGCCGAATTTCGGCCTGCAGATGTTCCGCGCCCAG
GACAGTGCCGAGCTGAAGCAAAGCCTGAACAGCCAGTTCAATGCCATGGCGGCGGTCGCCGAACGCCACGCCCCGGCGCT
GGAGTGGTTGCAGGCGCAGCAATCCAGCCTGGCCACCGCCGACTACAACGCGTTCGTGCGCTTCAGCGCGCTCAACGCCG
AACTGCAGAAATTCAAGGCCGACAACCCGGCGAGCACCGCGGCGCAGCTGGCGAAGCTGGTGGGTAACGATTTCAACCAG
ATGGACATCGGCAGTTGCGCCGACATTCTCAACGGCGTGCTCCTGCCGTCCAGCCGCAGCGACCTGGCGACGCGCCTGGT
GGACCTGCGCCAGGGTGCGCTCGGGCGCTGCCAGTCGTTGCAACAGCAGCAGGCGGCGCAGGCCTGGAAGGACCTCGCCG
ACTATTTCAACCAGTACCTCGCCGGACGCTTCCCGTTCGCCTACAGCCTGGAAGCGGCCGATGCCGAGCCGGGGCGGGTG
CGGCACCTGCTGAAACTGATGGAGACGCGCTTGCCGCAGGTGCGCGAAGGGCTGGCCCAGGTTCGCTCGCCGGACCTGCC
GGCCGCCGAGGACTTCGTCCGCCGCCTGGAGCAGGCGCAGCGCTGGCTCGGCCCGCTGTTCGAGCGCGACAAGTCGGGCC
TGCTCGGCGTCGCCCTGGACATCGACTGGCGCAGCGACCGCAGCCTGGAACGCGGCGCCGACCAGGTGATCGCCTGGAGC
CTGTACTCGGGCGACCAGGAAAGCCGTTTCCCCGGCGCGCAGGACAAGGGCCTGACCTGGAGTGTCGGCGATCCGCTGAA
AATCATGCTGCGCTGGGCGAAGAACGGCTCGCAGCGTCCGGCAGACGACCCGCGCCAGGCCAGCCTGGCGGTGGCCGACC
TCGAGGCCGGCTGGAGCTACCAGGGCTCCTGGGCGCTGCTGCGCATGATGCGCGCGCACTTCTCCCGGCAGCGTCCGCCG
AACGTCGACTACACGGAGTTTCCGCTGGTCCTGCAACTGCCGGTGTATGCGCCCTACAGCCCGGAAAACGAGGCGCGGAT
GTTCCTCCGCCTGTCGTTGATGAGCCTGGGCGGCAAGACCCCGCTGTCCATCCAGCCGCTGCCGGTGCGCGCGCCGCAAT
CGCCGTTCGCGACGCTGTTGCCGGCCACCGTCGCCAGCACCGGAGGTACCCCATGA