SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015319
Component protein type	PpkA
T6SS ID (Type)	T6SS01209 (Type Unknown)
Strain	Pseudomonas fluorescens strain MFE01
Replicon	chromosome
Sequence	Protein sequence (334 a.a.); Nucleotide sequence (1002 bp)
Reference	[1] Decoin V, Barbey C, Bergeau D, et al. A type VI secretion system is involved in Pseudomonas fluorescens bacterial competition. PLoS One. 2014 Feb 14;9(2):e89411. doi: 10.1371/journal.pone.0089411. eCollection 2014. PMID: 24551247 [2] Decoin V, Gallique M, Barbey C, et al. A Pseudomonas fluorescens type 6 secretion system is related to mucoidy, motility and bacterial competition. BMC Microbiol. 2015 Mar 26;15:72. doi: 10.1186/s12866-015-0405-9. PMID: 25886496

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	-
Coordinate (Strand)	1..1002 (+)
NCBI ID	AQM58484.1
GenBank	KX941493
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Pkinase	PF00069.27	4.6e-38	54..310

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-333	MSELESPIDDLLISEEQASNLTYFAFAKGNKAEPLLAPTKASIGALPDVLAGRYHLERLLGAGGMGAVYRARDLLHEQFGDPDPYIALKILSEEFAESPDASALLYSEFALTRRLRHDNVVRAHTFEVDTDCQRAFITMEYMRGLTLDKLLCERPLGLPWQELRDIVLPLLDTLAYAHRRGVLHGDLKPSNVMLSEDGLRLFDFGLGQAEEGTLPGLPHLSRERFNAWTPGYAAPELLEGQPLSASADVYGVACVIYELAGGKHPFRRLPSTQARDEHLERELRAPANLPQHGWQALRTALSFNPAERAITAQQLRDALGATSSWLQRLRLRA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9779	-	-

Protein sequence: 334 a.a.

						>T6CP015319 KX941493:1-1002 [Pseudomonas fluorescens] [PpkA]
MSELESPIDDLLISEEQASNLTYFAFAKGNKAEPLLAPTKASIGALPDVLAGRYHLERLLGAGGMGAVYRARDLLHEQFG
DPDPYIALKILSEEFAESPDASALLYSEFALTRRLRHDNVVRAHTFEVDTDCQRAFITMEYMRGLTLDKLLCERPLGLPW
QELRDIVLPLLDTLAYAHRRGVLHGDLKPSNVMLSEDGLRLFDFGLGQAEEGTLPGLPHLSRERFNAWTPGYAAPELLEG
QPLSASADVYGVACVIYELAGGKHPFRRLPSTQARDEHLERELRAPANLPQHGWQALRTALSFNPAERAITAQQLRDALG
ATSSWLQRLRLRA*					

Nucleotide sequence: 1002 bp

						>T6CP015319 KX941493:1-1002 [Pseudomonas fluorescens] [PpkA]
ATGAGTGAACTCGAATCGCCCATCGATGACCTGCTGATCAGCGAAGAACAGGCCAGCAACCTGACCTACTTCGCCTTCGC
CAAGGGCAACAAGGCCGAACCGTTGCTGGCGCCGACCAAGGCCAGCATCGGTGCGCTGCCGGATGTACTCGCCGGTCGCT
ACCACCTCGAGCGTCTGCTCGGGGCCGGTGGCATGGGCGCCGTTTACCGGGCGCGGGATCTGCTGCATGAGCAGTTCGGC
GATCCCGATCCTTACATCGCGCTGAAAATCCTCAGCGAAGAATTTGCCGAATCGCCGGACGCCAGTGCCTTGCTCTACAG
CGAGTTCGCCCTGACCCGACGCCTGCGCCACGACAATGTCGTGCGTGCACACACCTTTGAAGTCGACACCGACTGCCAGC
GGGCCTTCATCACCATGGAATACATGCGTGGCCTGACCCTGGACAAATTGCTCTGCGAGCGGCCCCTCGGCCTGCCGTGG
CAAGAATTGCGCGACATCGTCCTGCCGCTGCTCGACACGCTGGCCTACGCCCACCGTCGCGGCGTGCTGCACGGTGATCT
GAAACCGAGCAACGTCATGCTCAGCGAAGACGGCCTGCGCCTGTTCGACTTCGGTCTGGGACAGGCAGAGGAGGGCACCT
TGCCCGGTCTGCCGCACCTGAGCCGCGAGCGCTTCAACGCCTGGACCCCAGGCTACGCCGCCCCCGAACTGCTTGAGGGC
CAACCGTTGTCGGCCAGCGCGGACGTGTACGGCGTGGCCTGCGTGATCTATGAACTGGCGGGCGGCAAACACCCGTTCCG
CCGCTTACCCTCGACCCAGGCCCGCGACGAGCACCTGGAGCGCGAACTGCGCGCCCCAGCGAATCTACCCCAGCACGGCT
GGCAAGCGCTGCGAACCGCGCTGAGCTTCAACCCGGCAGAACGCGCCATCACTGCCCAACAATTGCGTGACGCCTTGGGC
GCCACTTCGTCCTGGCTGCAACGTCTGCGACTTCGGGCGTAA					