SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015318
Component protein type	PppA
T6SS ID (Type)	T6SS01209 (Type Unknown)
Strain	Pseudomonas fluorescens strain MFE01
Replicon	chromosome
Sequence	Protein sequence (243 a.a.); Nucleotide sequence (729 bp)
Reference	[1] Decoin V, Barbey C, Bergeau D, et al. A type VI secretion system is involved in Pseudomonas fluorescens bacterial competition. PLoS One. 2014 Feb 14;9(2):e89411. doi: 10.1371/journal.pone.0089411. eCollection 2014. PMID: 24551247 [2] Decoin V, Gallique M, Barbey C, et al. A Pseudomonas fluorescens type 6 secretion system is related to mucoidy, motility and bacterial competition. BMC Microbiol. 2015 Mar 26;15:72. doi: 10.1186/s12866-015-0405-9. PMID: 25886496

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	-
Coordinate (Strand)	1..729 (+)
NCBI ID	AQM58483.1
GenBank	KX941492
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
PP2C_2	PF13672.8	1.3e-13	19..203

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-242	MLVASPWRSAARTDPGKVRARNEDAFLDSPQHGLWVVADGMGGHQGGDIASQLIVASLAELPQHEDFDERLKAIRQCLHWLNRRLGQELTVTAGRHDSIMGSTVVALLVEGNRAACIWAGDSRCYMWRGQRLYQLSKDHSLQQQLIDEQQMSVEQAAAHPAAQALTRAVGAADTLTLDVLELEVYPGDVFLLCSDGLYQGLSSDALGNALSLSAPHVALERLFDGALRGAARDNLTAVVIRQ

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9756	-	-

Protein sequence: 243 a.a.

						>T6CP015318 KX941492:1-729 [Pseudomonas fluorescens] [PppA]
MLVASPWRSAARTDPGKVRARNEDAFLDSPQHGLWVVADGMGGHQGGDIASQLIVASLAELPQHEDFDERLKAIRQCLHW
LNRRLGQELTVTAGRHDSIMGSTVVALLVEGNRAACIWAGDSRCYMWRGQRLYQLSKDHSLQQQLIDEQQMSVEQAAAHP
AAQALTRAVGAADTLTLDVLELEVYPGDVFLLCSDGLYQGLSSDALGNALSLSAPHVALERLFDGALRGAARDNLTAVVI
RQ*					

Nucleotide sequence: 729 bp

						>T6CP015318 KX941492:1-729 [Pseudomonas fluorescens] [PppA]
ATGCTGGTGGCCAGTCCCTGGCGCAGCGCGGCGCGTACCGACCCGGGCAAGGTGCGGGCGCGCAACGAAGATGCCTTCCT
CGACTCGCCGCAGCATGGGCTGTGGGTGGTCGCGGACGGCATGGGCGGTCATCAGGGTGGCGACATCGCCAGCCAGTTGA
TCGTCGCCAGCCTGGCTGAGTTGCCGCAACACGAGGACTTCGACGAACGCCTCAAAGCCATCCGCCAGTGCCTGCACTGG
CTGAACCGGCGCTTGGGGCAGGAGTTGACCGTCACCGCCGGACGTCACGACAGCATCATGGGCAGCACCGTCGTCGCGCT
GCTGGTGGAAGGCAATCGCGCGGCCTGTATCTGGGCCGGCGACAGCCGTTGCTACATGTGGCGCGGGCAGCGTTTGTATC
AGCTGTCCAAGGACCATTCGCTGCAACAGCAGCTGATCGACGAGCAGCAAATGAGCGTCGAACAGGCGGCAGCGCACCCG
GCGGCTCAGGCACTCACCCGAGCCGTCGGCGCCGCCGACACACTGACCCTGGATGTGCTTGAACTCGAGGTCTATCCGGG
CGATGTGTTTTTGCTCTGCAGCGATGGTCTGTATCAGGGCCTGAGCAGCGATGCCCTCGGCAACGCCCTCAGCCTCAGCG
CGCCGCACGTGGCGCTGGAACGTTTGTTCGACGGCGCCCTGCGTGGCGCTGCGCGGGACAACCTGACTGCCGTGGTGATC
CGCCAATGA					