SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015317
Component protein type	TssM
T6SS ID (Type)	T6SS01209 (Type Unknown)
Strain	Pseudomonas fluorescens strain MFE01
Replicon	chromosome
Sequence	Protein sequence (1180 a.a.); Nucleotide sequence (3540 bp)
Reference	[1] Decoin V, Barbey C, Bergeau D, et al. A type VI secretion system is involved in Pseudomonas fluorescens bacterial competition. PLoS One. 2014 Feb 14;9(2):e89411. doi: 10.1371/journal.pone.0089411. eCollection 2014. PMID: 24551247 [2] Decoin V, Gallique M, Barbey C, et al. A Pseudomonas fluorescens type 6 secretion system is related to mucoidy, motility and bacterial competition. BMC Microbiol. 2015 Mar 26;15:72. doi: 10.1186/s12866-015-0405-9. PMID: 25886496

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	-
Coordinate (Strand)	1..3540 (+)
NCBI ID	AQM58482.1
GenBank	KX941491
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
IcmF-related_N	PF14331.8	8.3e-80	198..452
IcmF-related	PF06761.14	8.1e-74	502..792
IcmF_C	PF06744.14	1.7e-21	1054..1159

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-16	MKKFFKKVGAFLRQTW
Transmembrane helix	17-39	VWTLLLVLFVALLVWFVGPLLAV
Outside	40-48	DDYKFWESA
Transmembrane helix	49-71	TSRLLTISVLFLIWGLTMVFVSW
Inside	72-447	RAGIRKKAEEESEDGQDRIRREELIDEEQKELKARFKDALKTLKTSSLYRGRSERWRSDLPWYLLIGPQASGKTSLLDFSGLEFPINKIDRKLTRDTLGTRHCDWYFADHGVLIDTAGRYTTQPDAEVDGNAWTTLLELLRKRRRGRPLNGVLVTIPVETLTGGSEQDIETLARQVRSRLQDVHQKLHVDVPVYLVLSKADKLLGFDEFFDQLTREESDQVLGTSFRKDQVGTDVTVLRNEFEELLRRLNSQVIMRMHSERDTQRRGRILDFPHQLGQIGERLCLFVDMAFTGNRYQRATQLRGFYLTSAPHLTQEMDSTTAGIGASLGMNAGVLPTLRSGRSRFIHHLLSQVIFPEADLAGLDKRERSRIHWGQR
Transmembrane helix	448-470	ALYVGALAALALFGMLWAGGFSA
Outside	471-1179	NYERLENLRTLAQNWTQQRSAVTPRDDAMGVLKVLDTSYAATQVFPSKGDVSYHERGGLYQGEEINPVVKTAYERELEAQLLPRVATMLEGQIRANMKDRDRLLNSLRAYLMLNMKDRRDAAWLKDWVATDWSQRYTGNTAVQNGLNTHLERLLKQPFIYPLNDQLVTQARQVLRSESLANVVYRMLREQARNLPDYRFSQHLGPQGSLFIGTEYVIPGFYTQQGYQQYFSVQGAALVTDILRDNWVLGEGAGISDMDLRRLMVELEQLYFRDYANYWSEAVGQVALPPISDAGEGAEQLAGLTSANSPVLALLTEVRENTRFEAAADPVDEAGEAADALAGQKGNLGKVGKLAAAAADKASALNVAKNLPDTAKKSLQRRFEPLHRLLDDNNGPGADLTPAFSALNDLQLQLAGLARSSTPEQAAFEMAKTRMSGQRDALTNLRNASGRLPRPLSVWFNVLAEDSWRLVLNDAYQYLNGRYQNELYSVYGKTISKRYPFSASSTSDVAISDFREFFRAQGTVDRFFDSYMRPFVSGDPGNYRMRSVDGHSLPVSKVYLDQMAAAQTIRQSFFSINPAEPTVQFKLEPYTLDPAVSRSEFKFGDKTMEYRHGPILPMTFKWPTDAEDGRTSLVMDKMAGRPIGIEKNSGPWSLFRLFDLMQTEYLNGRDVLVLKADVGGLRANYLLTSQRTPNPFDMGVLRTFRMPVQL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9950	-	-

Protein sequence: 1180 a.a.

						>T6CP015317 KX941491:1-3540 [Pseudomonas fluorescens] [TssM]
MKKFFKKVGAFLRQTWVWTLLLVLFVALLVWFVGPLLAVDDYKFWESATSRLLTISVLFLIWGLTMVFVSWRAGIRKKAE
EESEDGQDRIRREELIDEEQKELKARFKDALKTLKTSSLYRGRSERWRSDLPWYLLIGPQASGKTSLLDFSGLEFPINKI
DRKLTRDTLGTRHCDWYFADHGVLIDTAGRYTTQPDAEVDGNAWTTLLELLRKRRRGRPLNGVLVTIPVETLTGGSEQDI
ETLARQVRSRLQDVHQKLHVDVPVYLVLSKADKLLGFDEFFDQLTREESDQVLGTSFRKDQVGTDVTVLRNEFEELLRRL
NSQVIMRMHSERDTQRRGRILDFPHQLGQIGERLCLFVDMAFTGNRYQRATQLRGFYLTSAPHLTQEMDSTTAGIGASLG
MNAGVLPTLRSGRSRFIHHLLSQVIFPEADLAGLDKRERSRIHWGQRALYVGALAALALFGMLWAGGFSANYERLENLRT
LAQNWTQQRSAVTPRDDAMGVLKVLDTSYAATQVFPSKGDVSYHERGGLYQGEEINPVVKTAYERELEAQLLPRVATMLE
GQIRANMKDRDRLLNSLRAYLMLNMKDRRDAAWLKDWVATDWSQRYTGNTAVQNGLNTHLERLLKQPFIYPLNDQLVTQA
RQVLRSESLANVVYRMLREQARNLPDYRFSQHLGPQGSLFIGTEYVIPGFYTQQGYQQYFSVQGAALVTDILRDNWVLGE
GAGISDMDLRRLMVELEQLYFRDYANYWSEAVGQVALPPISDAGEGAEQLAGLTSANSPVLALLTEVRENTRFEAAADPV
DEAGEAADALAGQKGNLGKVGKLAAAAADKASALNVAKNLPDTAKKSLQRRFEPLHRLLDDNNGPGADLTPAFSALNDLQ
LQLAGLARSSTPEQAAFEMAKTRMSGQRDALTNLRNASGRLPRPLSVWFNVLAEDSWRLVLNDAYQYLNGRYQNELYSVY
GKTISKRYPFSASSTSDVAISDFREFFRAQGTVDRFFDSYMRPFVSGDPGNYRMRSVDGHSLPVSKVYLDQMAAAQTIRQ
SFFSINPAEPTVQFKLEPYTLDPAVSRSEFKFGDKTMEYRHGPILPMTFKWPTDAEDGRTSLVMDKMAGRPIGIEKNSGP
WSLFRLFDLMQTEYLNGRDVLVLKADVGGLRANYLLTSQRTPNPFDMGVLRTFRMPVQL*					

Nucleotide sequence: 3540 bp

						>T6CP015317 KX941491:1-3540 [Pseudomonas fluorescens] [TssM]
ATGAAAAAGTTTTTCAAGAAAGTCGGCGCCTTCCTGCGCCAGACCTGGGTCTGGACTCTGCTGCTGGTGCTGTTCGTGGC
GCTGCTGGTGTGGTTCGTCGGTCCGTTGTTGGCGGTCGATGATTACAAGTTCTGGGAAAGCGCGACCTCGCGTCTGCTGA
CCATCAGCGTGCTGTTCCTGATCTGGGGCCTGACCATGGTCTTCGTCAGCTGGCGCGCGGGTATCCGCAAGAAAGCCGAG
GAAGAAAGCGAGGACGGCCAGGACCGCATTCGTCGCGAAGAGCTGATCGACGAGGAACAGAAAGAGTTGAAGGCGCGTTT
CAAGGACGCCCTGAAAACCCTGAAGACCTCGAGCCTGTATCGCGGTCGCAGCGAGCGCTGGCGCAGTGACTTGCCGTGGT
ACCTGCTGATTGGTCCGCAGGCCTCGGGTAAGACCAGCCTGCTGGACTTTTCCGGTCTGGAATTCCCGATCAACAAGATC
GACCGCAAGCTGACCCGCGACACCCTCGGCACCCGTCATTGCGACTGGTATTTCGCCGATCACGGTGTACTGATCGACAC
CGCCGGGCGTTACACCACTCAACCGGACGCGGAAGTCGACGGCAACGCCTGGACCACCCTGCTGGAACTGCTGCGCAAGC
GTCGTCGCGGCCGTCCGTTGAACGGCGTGCTGGTGACCATTCCGGTGGAAACCCTCACCGGCGGCAGCGAGCAGGACATC
GAGACCCTCGCGCGCCAGGTGCGCAGCCGTCTGCAGGATGTGCATCAGAAACTCCACGTCGACGTGCCGGTGTATCTGGT
CCTGAGCAAGGCTGACAAGCTGCTCGGCTTCGACGAGTTCTTCGATCAACTGACCCGCGAAGAAAGCGATCAGGTGCTCG
GCACCAGTTTCCGCAAGGATCAGGTCGGCACCGACGTCACGGTTCTGCGCAACGAGTTCGAAGAGCTGCTGCGCCGCCTC
AACAGCCAAGTGATCATGCGCATGCACTCCGAGCGCGACACTCAGCGCCGTGGCCGCATCCTCGACTTCCCGCATCAGTT
GGGGCAGATCGGCGAGCGTCTGTGCCTGTTCGTCGACATGGCGTTCACCGGCAACCGCTATCAGCGCGCCACGCAACTGC
GCGGCTTCTACCTGACCAGCGCGCCGCACCTGACCCAGGAAATGGATTCGACCACCGCCGGCATCGGCGCCAGCCTGGGC
ATGAACGCCGGCGTGCTGCCGACTCTGCGCAGCGGTCGTTCGCGATTCATCCACCACTTGCTCAGCCAAGTGATTTTCCC
CGAGGCCGATCTGGCCGGTCTGGACAAGCGCGAGCGCAGCCGCATTCATTGGGGCCAGCGTGCGCTGTACGTCGGCGCTC
TGGCGGCGCTGGCACTGTTCGGTATGCTCTGGGCCGGTGGTTTCTCGGCCAACTACGAGCGTCTGGAAAATCTGCGCACG
CTGGCGCAGAACTGGACCCAGCAACGCTCGGCCGTCACGCCGCGTGATGACGCCATGGGCGTGCTGAAAGTGCTCGACAC
CAGCTACGCCGCGACCCAGGTGTTCCCGAGCAAGGGCGACGTCTCGTACCACGAACGTGGCGGCCTGTATCAGGGCGAAG
AGATCAACCCGGTGGTGAAAACCGCCTACGAGCGTGAGCTGGAAGCGCAGTTGCTGCCACGGGTGGCGACCATGCTCGAA
GGGCAGATCCGCGCCAACATGAAGGACCGCGATCGCCTGCTCAACAGCCTGCGTGCGTACCTGATGCTGAACATGAAGGA
CCGTCGCGACGCGGCGTGGCTCAAGGACTGGGTCGCCACTGACTGGTCGCAACGCTACACCGGTAACACCGCGGTGCAGA
ACGGTCTGAATACGCACCTCGAGCGTTTGCTCAAGCAGCCGTTCATTTATCCGCTGAACGATCAACTGGTGACGCAGGCG
CGTCAGGTCCTGCGCAGCGAATCGTTGGCCAACGTGGTTTACCGCATGCTCCGCGAGCAGGCGCGCAACCTGCCGGACTA
CCGCTTCAGCCAGCACCTCGGCCCGCAAGGTTCGTTGTTCATCGGCACTGAATACGTGATCCCGGGTTTCTACACCCAGC
AGGGTTATCAGCAGTATTTCTCGGTGCAGGGCGCCGCGCTGGTCACCGACATCCTGCGCGACAACTGGGTGCTGGGCGAA
GGCGCGGGCATCAGCGACATGGATTTGCGTCGCCTGATGGTCGAGCTTGAGCAGCTGTACTTCCGCGACTACGCCAACTA
CTGGAGCGAAGCCGTTGGCCAGGTCGCATTGCCGCCAATCAGCGACGCTGGCGAAGGCGCCGAGCAACTGGCGGGCCTGA
CTTCGGCCAACTCGCCGGTCCTCGCATTGCTCACCGAAGTGCGTGAAAACACTCGCTTCGAAGCCGCGGCTGATCCGGTC
GATGAAGCCGGCGAAGCCGCCGATGCGCTGGCCGGGCAGAAAGGCAACCTGGGCAAGGTCGGCAAACTCGCTGCGGCTGC
CGCCGACAAGGCTTCGGCGCTGAACGTGGCGAAGAACCTGCCGGACACCGCGAAGAAATCCCTGCAACGCCGCTTCGAAC
CGCTGCATCGTCTGCTCGATGACAACAACGGCCCGGGCGCTGACCTGACGCCAGCGTTCAGCGCGCTCAACGACCTGCAA
CTGCAACTGGCCGGTCTGGCCCGTTCCAGCACGCCGGAGCAAGCCGCGTTCGAGATGGCCAAGACGCGCATGAGCGGCCA
GCGTGATGCGCTGACCAACCTGCGCAATGCCTCCGGTCGTCTGCCACGTCCACTGAGCGTGTGGTTCAACGTGCTGGCCG
AAGACTCGTGGCGCCTGGTGCTCAACGATGCCTATCAATACCTGAACGGCCGTTATCAGAACGAGCTGTACAGCGTGTAC
GGCAAAACCATCAGCAAGCGCTATCCGTTCAGCGCCAGCAGCACCAGCGACGTGGCGATCAGCGACTTCCGTGAGTTCTT
CCGTGCTCAAGGTACCGTCGACCGCTTCTTCGACAGCTACATGCGTCCATTCGTCAGCGGCGATCCGGGCAACTACCGCA
TGCGCAGCGTCGACGGTCACAGCCTGCCGGTATCGAAGGTCTACCTCGATCAGATGGCGGCGGCGCAGACCATTCGCCAG
AGCTTCTTCTCGATCAACCCGGCCGAGCCGACCGTGCAGTTCAAGCTCGAGCCGTACACCCTCGATCCGGCGGTCAGCCG
CTCCGAATTCAAGTTCGGCGACAAGACCATGGAATACCGCCACGGCCCGATCCTGCCAATGACCTTCAAGTGGCCGACCG
ATGCTGAAGACGGTCGCACCAGTCTGGTCATGGACAAGATGGCCGGACGCCCGATCGGGATCGAGAAGAATTCCGGCCCA
TGGTCGCTGTTCCGTCTGTTCGACCTGATGCAGACCGAGTACCTGAACGGTCGTGACGTGCTGGTACTGAAAGCCGACGT
GGGCGGCCTGCGCGCCAACTACCTGCTCACCAGCCAGCGCACGCCGAACCCGTTCGACATGGGCGTGCTGCGCACCTTCC
GTATGCCGGTGCAGCTCTGA					