SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015317
Component protein type	TssM
T6SS ID (Type)	T6SS01209 (Type Unknown)
Strain	Pseudomonas fluorescens strain MFE01
Replicon	chromosome
Sequence	Protein sequence (1180 a.a.); Nucleotide sequence (3540 bp)
Reference	[1] Decoin V, Barbey C, Bergeau D, et al. A type VI secretion system is involved in Pseudomonas fluorescens bacterial competition. PLoS One. 2014 Feb 14;9(2):e89411. doi: 10.1371/journal.pone.0089411. eCollection 2014. PMID: 24551247 [2] Decoin V, Gallique M, Barbey C, et al. A Pseudomonas fluorescens type 6 secretion system is related to mucoidy, motility and bacterial competition. BMC Microbiol. 2015 Mar 26;15:72. doi: 10.1186/s12866-015-0405-9. PMID: 25886496

Experimental investigation has been performed on this T6SS

External database links

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Prediction	Region	Sequence
Inside	1-16	MKKFFKKVGAFLRQTW
Transmembrane helix	17-39	VWTLLLVLFVALLVWFVGPLLAV
Outside	40-48	DDYKFWESA
Transmembrane helix	49-71	TSRLLTISVLFLIWGLTMVFVSW
Inside	72-447	RAGIRKKAEEESEDGQDRIRREELIDEEQKELKARFKDALKTLKTSSLYRGRSERWRSDLPWYLLIGPQASGKTSLLDFSGLEFPINKIDRKLTRDTLGTRHCDWYFADHGVLIDTAGRYTTQPDAEVDGNAWTTLLELLRKRRRGRPLNGVLVTIPVETLTGGSEQDIETLARQVRSRLQDVHQKLHVDVPVYLVLSKADKLLGFDEFFDQLTREESDQVLGTSFRKDQVGTDVTVLRNEFEELLRRLNSQVIMRMHSERDTQRRGRILDFPHQLGQIGERLCLFVDMAFTGNRYQRATQLRGFYLTSAPHLTQEMDSTTAGIGASLGMNAGVLPTLRSGRSRFIHHLLSQVIFPEADLAGLDKRERSRIHWGQR
Transmembrane helix	448-470	ALYVGALAALALFGMLWAGGFSA
Outside	471-1179	NYERLENLRTLAQNWTQQRSAVTPRDDAMGVLKVLDTSYAATQVFPSKGDVSYHERGGLYQGEEINPVVKTAYERELEAQLLPRVATMLEGQIRANMKDRDRLLNSLRAYLMLNMKDRRDAAWLKDWVATDWSQRYTGNTAVQNGLNTHLERLLKQPFIYPLNDQLVTQARQVLRSESLANVVYRMLREQARNLPDYRFSQHLGPQGSLFIGTEYVIPGFYTQQGYQQYFSVQGAALVTDILRDNWVLGEGAGISDMDLRRLMVELEQLYFRDYANYWSEAVGQVALPPISDAGEGAEQLAGLTSANSPVLALLTEVRENTRFEAAADPVDEAGEAADALAGQKGNLGKVGKLAAAAADKASALNVAKNLPDTAKKSLQRRFEPLHRLLDDNNGPGADLTPAFSALNDLQLQLAGLARSSTPEQAAFEMAKTRMSGQRDALTNLRNASGRLPRPLSVWFNVLAEDSWRLVLNDAYQYLNGRYQNELYSVYGKTISKRYPFSASSTSDVAISDFREFFRAQGTVDRFFDSYMRPFVSGDPGNYRMRSVDGHSLPVSKVYLDQMAAAQTIRQSFFSINPAEPTVQFKLEPYTLDPAVSRSEFKFGDKTMEYRHGPILPMTFKWPTDAEDGRTSLVMDKMAGRPIGIEKNSGPWSLFRLFDLMQTEYLNGRDVLVLKADVGGLRANYLLTSQRTPNPFDMGVLRTFRMPVQL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9950	-	-

Protein sequence: 1180 a.a.

Nucleotide sequence: 3540 bp