SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015316
Component protein type	TssL
T6SS ID (Type)	T6SS01209 (Type Unknown)
Strain	Pseudomonas fluorescens strain MFE01
Replicon	chromosome
Sequence	Protein sequence (292 a.a.); Nucleotide sequence (876 bp)
Reference	[1] Decoin V, Barbey C, Bergeau D, et al. A type VI secretion system is involved in Pseudomonas fluorescens bacterial competition. PLoS One. 2014 Feb 14;9(2):e89411. doi: 10.1371/journal.pone.0089411. eCollection 2014. PMID: 24551247 [2] Decoin V, Gallique M, Barbey C, et al. A Pseudomonas fluorescens type 6 secretion system is related to mucoidy, motility and bacterial competition. BMC Microbiol. 2015 Mar 26;15:72. doi: 10.1186/s12866-015-0405-9. PMID: 25886496

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	-
Coordinate (Strand)	1..876 (+)
NCBI ID	AQM58481.1
GenBank	KX941490
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	1.6e-66	63..264

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-243	MIKETDYNQDDKTVLLDRQGHGPASSPLTDFAAPPRFEQLEERMIYAARLRPAEAFNISLNSLVAASSDLLSEVVRLKHSETREDLYALNERLTAGLKLFEVRALHNGAESSQVMAARYVLCTVVDEAVVTTPWGNESEWSQMSLLSSFHNETFGGEKFFQLLDRLSKNPIKHLPMLELMYLCLSLGFEGKYRVQARGMLELEGIRDALYRQIRQLRGDVPRELSPHWEGLNDQRRHLVRIVP
Transmembrane helix	244-266	AWMVVLFTLVCLVVMYSGFAWVL
Outside	267-291	SEQRDTVLHPYQPLDPAAVQPQAQP

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9795	-	-

Protein sequence: 292 a.a.

						>T6CP015316 KX941490:1-876 [Pseudomonas fluorescens] [TssL]
MIKETDYNQDDKTVLLDRQGHGPASSPLTDFAAPPRFEQLEERMIYAARLRPAEAFNISLNSLVAASSDLLSEVVRLKHS
ETREDLYALNERLTAGLKLFEVRALHNGAESSQVMAARYVLCTVVDEAVVTTPWGNESEWSQMSLLSSFHNETFGGEKFF
QLLDRLSKNPIKHLPMLELMYLCLSLGFEGKYRVQARGMLELEGIRDALYRQIRQLRGDVPRELSPHWEGLNDQRRHLVR
IVPAWMVVLFTLVCLVVMYSGFAWVLSEQRDTVLHPYQPLDPAAVQPQAQP*					

Nucleotide sequence: 876 bp

						>T6CP015316 KX941490:1-876 [Pseudomonas fluorescens] [TssL]
ATGATCAAGGAAACGGATTACAACCAGGACGACAAAACCGTCCTGCTCGACCGTCAGGGCCACGGACCGGCATCGAGTCC
GCTGACCGATTTCGCCGCGCCGCCGCGTTTCGAGCAACTGGAAGAACGCATGATCTACGCCGCGCGCCTGCGTCCGGCCG
AGGCGTTCAACATCAGCCTCAACTCGCTGGTCGCGGCCTCGTCCGACCTGCTCTCGGAAGTGGTGCGTCTCAAGCACAGC
GAAACCCGCGAAGACCTGTACGCACTCAATGAGCGGCTGACCGCCGGGCTGAAATTGTTCGAAGTACGCGCCCTGCACAA
CGGCGCCGAAAGCAGCCAGGTGATGGCCGCGCGTTACGTGCTCTGCACCGTGGTCGACGAAGCCGTCGTGACCACGCCGT
GGGGCAACGAAAGCGAGTGGTCGCAGATGAGCCTGCTGAGCAGCTTCCACAACGAAACCTTCGGCGGCGAGAAGTTTTTC
CAGCTGCTCGATCGGCTGTCGAAAAACCCGATCAAGCACCTGCCGATGCTCGAACTGATGTACCTGTGCCTGTCCCTCGG
TTTCGAGGGCAAGTACCGCGTCCAGGCGCGCGGCATGCTCGAGCTTGAAGGCATCCGCGACGCCTTGTATCGGCAGATCC
GTCAGTTGCGTGGCGACGTGCCGCGCGAGTTGTCGCCGCACTGGGAAGGCCTCAACGACCAGCGCCGCCACCTGGTGCGC
ATCGTGCCGGCGTGGATGGTGGTGCTCTTTACTCTGGTCTGCTTGGTGGTGATGTACTCGGGCTTCGCCTGGGTTCTGAG
CGAACAACGCGACACCGTTCTGCATCCTTATCAGCCGCTTGATCCAGCCGCGGTACAACCGCAGGCGCAGCCGTAA					