SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015314
Component protein type	TssJ
T6SS ID (Type)	T6SS01209 (Type Unknown)
Strain	Pseudomonas fluorescens strain MFE01
Replicon	chromosome
Sequence	Protein sequence (167 a.a.); Nucleotide sequence (501 bp)
Reference	[1] Decoin V, Barbey C, Bergeau D, et al. A type VI secretion system is involved in Pseudomonas fluorescens bacterial competition. PLoS One. 2014 Feb 14;9(2):e89411. doi: 10.1371/journal.pone.0089411. eCollection 2014. PMID: 24551247 [2] Decoin V, Gallique M, Barbey C, et al. A Pseudomonas fluorescens type 6 secretion system is related to mucoidy, motility and bacterial competition. BMC Microbiol. 2015 Mar 26;15:72. doi: 10.1186/s12866-015-0405-9. PMID: 25886496

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	-
Coordinate (Strand)	1..501 (+)
NCBI ID	AQM58479.1
GenBank	KX941488
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	3.1e-39	34..151

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-6	MSRRST
Transmembrane helix	7-29	AFFKTLTALTVLVLLAGCSSLSP
Outside	30-166	YSKVTKINLKLTGSDQLNPDLNGRPSPIVVRLFELKHPVTFENADFFSLYERAKESLNPDLVASEELELRPGETVELKLSVEEGSRYVGILAAYRDLPDTQWRHTVQITPLELTEADLTLDQAGIRNTQQVLAKADD

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9979	pos: 23-24. LAG-CS.	MSRRSTAFFKTLTALTVLVLLAG

Protein sequence: 167 a.a.

						>T6CP015314 KX941488:1-501 [Pseudomonas fluorescens] [TssJ]
MSRRSTAFFKTLTALTVLVLLAGCSSLSPYSKVTKINLKLTGSDQLNPDLNGRPSPIVVRLFELKHPVTFENADFFSLYE
RAKESLNPDLVASEELELRPGETVELKLSVEEGSRYVGILAAYRDLPDTQWRHTVQITPLELTEADLTLDQAGIRNTQQV
LAKADD*					

Nucleotide sequence: 501 bp

						>T6CP015314 KX941488:1-501 [Pseudomonas fluorescens] [TssJ]
ATGTCTCGCCGCTCGACCGCTTTTTTCAAGACGCTGACTGCGCTCACCGTGCTGGTGCTGCTGGCCGGTTGCTCGTCGCT
GTCGCCGTACTCGAAAGTGACCAAGATCAATCTGAAACTGACCGGCAGCGATCAGCTCAACCCGGACCTCAACGGGCGGC
CGTCGCCGATCGTCGTGCGCCTGTTCGAACTCAAGCACCCGGTGACCTTCGAGAACGCTGATTTCTTCAGCCTCTACGAA
CGCGCCAAGGAATCCCTCAACCCGGATCTGGTGGCCAGCGAAGAACTCGAACTGCGCCCGGGTGAAACCGTGGAACTGAA
ACTCAGCGTGGAGGAGGGCAGCCGTTACGTGGGCATCCTTGCTGCCTATCGCGACCTGCCGGATACCCAGTGGCGCCACA
CCGTGCAGATCACTCCGCTGGAACTCACCGAGGCTGATCTGACCCTCGATCAGGCCGGTATCCGCAACACCCAACAAGTG
CTCGCCAAGGCGGATGACTGA					