SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015313
Component protein type	FHA
T6SS ID (Type)	T6SS01209 (Type Unknown)
Strain	Pseudomonas fluorescens strain MFE01
Replicon	chromosome
Sequence	Protein sequence (399 a.a.); Nucleotide sequence (1197 bp)
Reference	[1] Decoin V, Barbey C, Bergeau D, et al. A type VI secretion system is involved in Pseudomonas fluorescens bacterial competition. PLoS One. 2014 Feb 14;9(2):e89411. doi: 10.1371/journal.pone.0089411. eCollection 2014. PMID: 24551247 [2] Decoin V, Gallique M, Barbey C, et al. A Pseudomonas fluorescens type 6 secretion system is related to mucoidy, motility and bacterial competition. BMC Microbiol. 2015 Mar 26;15:72. doi: 10.1186/s12866-015-0405-9. PMID: 25886496

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	-
Coordinate (Strand)	1..1197 (+)
NCBI ID	AQM58478.1
GenBank	KX941487
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
FHA	PF00498.28	7.8e-11	29..97

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-398	MELVFEMLNTKQFVPTELCQRTFKQAGGVIGRGEDCDWIIPDRKRHLSNHHAIVSYREGSFFLTDTSSNGVQDGSSGARLHKGEPVRIEHGSTYILGDFEIRARLVRDPATFDGEVGRPRAAGSIIPDDAFLDLDPLNALEQQERVYSEIDELLSPTAKPEDSRQRADYARIDMESLMVPELIAAPVEPEPAPVPKAVERQSEGFWEHFGAALGVDVKGLSHDEREALALNAARLLRQSIGGLQQSLRTRSELKNELRLAQTTVQGTNKNPLKFAVDPSEALQILLQPSKPGHLPAEQAISRAFRDLQAHQVALLTASRAAVRGTLEHFSPEQLTLRFERDSKPLIATSGGRWRAFGRYHQALRQDDDWSERLLARDFAQAYEEQIRLISTLHTDHQG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9851	-	-

Protein sequence: 399 a.a.

						>T6CP015313 KX941487:1-1197 [Pseudomonas fluorescens] [FHA]
MELVFEMLNTKQFVPTELCQRTFKQAGGVIGRGEDCDWIIPDRKRHLSNHHAIVSYREGSFFLTDTSSNGVQDGSSGARL
HKGEPVRIEHGSTYILGDFEIRARLVRDPATFDGEVGRPRAAGSIIPDDAFLDLDPLNALEQQERVYSEIDELLSPTAKP
EDSRQRADYARIDMESLMVPELIAAPVEPEPAPVPKAVERQSEGFWEHFGAALGVDVKGLSHDEREALALNAARLLRQSI
GGLQQSLRTRSELKNELRLAQTTVQGTNKNPLKFAVDPSEALQILLQPSKPGHLPAEQAISRAFRDLQAHQVALLTASRA
AVRGTLEHFSPEQLTLRFERDSKPLIATSGGRWRAFGRYHQALRQDDDWSERLLARDFAQAYEEQIRLISTLHTDHQG*					

Nucleotide sequence: 1197 bp

						>T6CP015313 KX941487:1-1197 [Pseudomonas fluorescens] [FHA]
ATGGAATTGGTTTTCGAAATGCTGAACACCAAGCAGTTCGTGCCCACCGAGTTGTGCCAGCGGACCTTCAAACAGGCCGG
TGGCGTGATCGGGCGCGGCGAGGACTGCGACTGGATCATCCCTGACCGCAAGCGGCACCTGTCCAATCACCACGCGATTG
TCAGCTACCGCGAGGGCTCGTTCTTCCTCACCGATACCAGCAGCAACGGTGTCCAGGACGGCAGCAGCGGCGCGCGCCTG
CACAAGGGCGAGCCGGTGCGTATCGAGCACGGCAGCACCTACATTCTCGGCGACTTCGAGATCCGTGCGCGGCTGGTTCG
TGACCCGGCGACCTTCGACGGCGAAGTCGGCCGCCCGCGCGCCGCTGGCAGCATCATTCCGGACGACGCGTTCCTCGACC
TCGACCCGCTCAACGCCCTCGAACAGCAAGAGCGCGTGTACTCGGAAATCGACGAACTGCTGTCGCCTACCGCCAAGCCG
GAAGACTCCCGTCAACGTGCCGACTACGCGCGCATCGACATGGAAAGCCTGATGGTCCCGGAGCTGATCGCCGCCCCGGT
CGAGCCTGAGCCGGCGCCTGTACCGAAAGCCGTCGAGCGTCAGAGCGAAGGTTTCTGGGAGCACTTCGGCGCGGCGCTGG
GCGTCGACGTCAAAGGCCTCAGCCACGACGAACGCGAAGCGCTGGCGCTGAACGCTGCGCGGCTGCTGCGCCAGAGCATC
GGCGGTTTGCAGCAAAGCCTGCGTACCCGCTCCGAGCTGAAAAACGAATTGCGTTTGGCCCAGACCACCGTGCAAGGCAC
CAACAAGAACCCGCTGAAATTCGCCGTCGATCCGAGCGAAGCACTGCAGATTCTGTTGCAGCCAAGCAAGCCTGGCCATC
TGCCCGCCGAGCAAGCTATCTCCCGTGCATTCCGTGATTTGCAGGCGCACCAAGTGGCACTGCTGACCGCCAGTCGCGCC
GCTGTGCGTGGCACGCTGGAGCACTTCTCGCCAGAGCAGCTGACCCTGCGTTTCGAGCGCGACAGCAAGCCGTTGATCGC
CACGTCGGGCGGGCGCTGGAGAGCGTTCGGCCGCTATCACCAGGCACTGCGTCAGGACGATGACTGGAGCGAGCGTCTGC
TGGCCCGCGACTTCGCCCAGGCCTACGAAGAACAGATCCGCCTGATCTCCACCCTCCACACCGACCACCAAGGATGA					