SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015309
Component protein type	TssG
T6SS ID (Type)	T6SS01209 (Type Unknown)
Strain	Pseudomonas fluorescens strain MFE01
Replicon	chromosome
Sequence	Protein sequence (336 a.a.); Nucleotide sequence (1008 bp)
Reference	[1] Decoin V, Barbey C, Bergeau D, et al. A type VI secretion system is involved in Pseudomonas fluorescens bacterial competition. PLoS One. 2014 Feb 14;9(2):e89411. doi: 10.1371/journal.pone.0089411. eCollection 2014. PMID: 24551247 [2] Decoin V, Gallique M, Barbey C, et al. A Pseudomonas fluorescens type 6 secretion system is related to mucoidy, motility and bacterial competition. BMC Microbiol. 2015 Mar 26;15:72. doi: 10.1186/s12866-015-0405-9. PMID: 25886496

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	-
Coordinate (Strand)	1..1008 (+)
NCBI ID	AQM58474.1
GenBank	KX941483
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	2.5e-87	20..320

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-335	MDTTYGPAAPALSGLTKVIREYSLFQAVLLVIDRLREAHPHLSEDDLYDQVEFQANPSLGFPRSDVDRVEFFEEHGKMRARVRFNLIGLVGSGSPLPAFYGEQALGDSEDGNPTRNFLDLFHHRLQRLMLPIWRKYRYRASFQSGAIDPFSAQLFALIGLGGDEIRKAKELNWKRLLPYLGLLSLRAHSAALIEAVLRYYFKHEDLVIEQCIERRVEILDEQRNRLGRANSVLGEDLVLGEHVRDRSGKFRIHITELDWQRFHEFLPIGFGYQPLCALVRFTLRDPLDYDIRLVLRQEEIRELRIGEQNACRLGWTSWLGREKADGVVTLGSKIH

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9885	-	-

Protein sequence: 336 a.a.

						>T6CP015309 KX941483:1-1008 [Pseudomonas fluorescens] [TssG]
MDTTYGPAAPALSGLTKVIREYSLFQAVLLVIDRLREAHPHLSEDDLYDQVEFQANPSLGFPRSDVDRVEFFEEHGKMRA
RVRFNLIGLVGSGSPLPAFYGEQALGDSEDGNPTRNFLDLFHHRLQRLMLPIWRKYRYRASFQSGAIDPFSAQLFALIGL
GGDEIRKAKELNWKRLLPYLGLLSLRAHSAALIEAVLRYYFKHEDLVIEQCIERRVEILDEQRNRLGRANSVLGEDLVLG
EHVRDRSGKFRIHITELDWQRFHEFLPIGFGYQPLCALVRFTLRDPLDYDIRLVLRQEEIRELRIGEQNACRLGWTSWLG
REKADGVVTLGSKIH*					

Nucleotide sequence: 1008 bp

						>T6CP015309 KX941483:1-1008 [Pseudomonas fluorescens] [TssG]
ATGGACACCACGTATGGGCCTGCAGCCCCTGCTTTAAGCGGGCTGACCAAGGTAATACGCGAGTACTCGCTGTTTCAGGC
CGTGCTGCTGGTGATCGACCGGCTGCGCGAGGCACACCCGCATCTGAGCGAAGACGATTTGTACGATCAGGTCGAGTTCC
AGGCCAACCCGAGCCTGGGCTTTCCGCGCAGCGACGTCGATCGCGTCGAGTTTTTCGAAGAGCACGGCAAGATGCGCGCG
CGCGTGCGTTTCAACCTGATCGGCCTGGTCGGCTCCGGTTCGCCGTTGCCGGCGTTCTATGGCGAGCAGGCCCTGGGCGA
CAGCGAGGACGGCAACCCGACGCGCAACTTCCTCGACCTGTTCCACCATCGTCTGCAACGGCTGATGCTGCCGATCTGGC
GCAAGTATCGCTACCGCGCGAGCTTCCAGAGCGGCGCGATTGACCCGTTCTCGGCGCAACTTTTTGCGCTGATCGGCCTG
GGTGGCGACGAGATCCGCAAGGCCAAGGAACTCAACTGGAAACGCTTGCTGCCGTACCTCGGCCTGCTCAGTTTGCGCGC
GCACTCGGCGGCGCTGATCGAAGCCGTGCTGCGTTACTACTTCAAGCACGAAGACCTGGTCATCGAGCAGTGCATCGAGC
GCCGCGTGGAAATTCTCGACGAGCAGCGCAATCGTTTGGGCCGCGCCAACAGCGTACTCGGCGAAGACCTGGTGCTGGGC
GAGCACGTGCGCGATCGCAGCGGCAAATTCCGCATTCACATTACCGAACTCGACTGGCAGCGATTCCATGAATTCCTGCC
GATCGGTTTCGGCTACCAGCCGCTCTGCGCGCTGGTGCGGTTCACTTTGCGTGACCCGCTCGATTACGACATCCGCCTGG
TCCTGCGCCAGGAAGAAATCCGCGAACTGCGCATCGGTGAGCAGAACGCCTGTCGCCTCGGTTGGACCAGTTGGCTGGGC
CGCGAAAAAGCGGACGGCGTGGTGACCCTGGGCAGCAAAATTCATTAA					