Detailed information of component protein
Summary
| Component ID | T6CP015303 |
| Component protein type | TssE |
| T6SS ID (Type) | T6SS01207  (Type iv) |
| Strain | Candidatus Amoebophilus asiaticus 5a2 |
| Replicon | chromosome |
| Sequence | Protein sequence (132 a.a.); Nucleotide sequence (396 bp) |
| Reference | - |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | AASI_RS05530 |
| Coordinate (Strand) | 1394004..1394399 (+) |
| NCBI ID | WP_012473185.1 |
| RefSeq | NC_010830 |
| Uniprot ID | B3ET75_AMOA5 |
| KEGG ID | aas:Aasi_1082 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| GPW_gp25 | PF04965.16 | 1.5e-20 | 30..119 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-131 | MIEESMFLGVGWNFPPSFDRYSKSIEMVRDEIDIHQSLQVLFTTTPGERTMELDYGCDLSPLAFQRLDLNLKTFMINNIKDAIARCEPRIRVKEVKLEEEDNISGLVNIYVSYIIKSTNMPGNLVYAHSFE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9948 | - | - |
Protein sequence: 132 a.a.
>T6CP015303 NC_010830:1394004-1394399 [Candidatus Amoebophilus asiaticus 5a2] [TssE]
MIEESMFLGVGWNFPPSFDRYSKSIEMVRDEIDIHQSLQVLFTTTPGERTMELDYGCDLSPLAFQRLDLNLKTFMINNIK
DAIARCEPRIRVKEVKLEEEDNISGLVNIYVSYIIKSTNMPGNLVYAHSFE*
MIEESMFLGVGWNFPPSFDRYSKSIEMVRDEIDIHQSLQVLFTTTPGERTMELDYGCDLSPLAFQRLDLNLKTFMINNIK
DAIARCEPRIRVKEVKLEEEDNISGLVNIYVSYIIKSTNMPGNLVYAHSFE*
Nucleotide sequence: 396 bp
>T6CP015303 NC_010830:1394004-1394399 [Candidatus Amoebophilus asiaticus 5a2] [TssE]
ATGATAGAAGAAAGCATGTTTTTGGGAGTTGGATGGAACTTCCCTCCTTCCTTTGATAGATATAGTAAATCAATAGAAAT
GGTACGAGATGAAATAGATATTCATCAAAGCTTACAGGTATTATTCACAACTACACCTGGTGAAAGAACTATGGAGTTGG
ATTATGGTTGTGATTTGAGTCCCCTAGCCTTCCAGCGACTAGATCTGAATCTAAAAACCTTTATGATCAACAATATTAAA
GATGCGATTGCCCGTTGTGAACCAAGAATACGCGTCAAGGAAGTAAAGCTTGAGGAAGAAGATAATATATCCGGCCTGGT
TAATATATACGTAAGCTATATTATAAAGTCTACTAATATGCCAGGGAATTTAGTTTATGCTCACTCTTTTGAATAA
ATGATAGAAGAAAGCATGTTTTTGGGAGTTGGATGGAACTTCCCTCCTTCCTTTGATAGATATAGTAAATCAATAGAAAT
GGTACGAGATGAAATAGATATTCATCAAAGCTTACAGGTATTATTCACAACTACACCTGGTGAAAGAACTATGGAGTTGG
ATTATGGTTGTGATTTGAGTCCCCTAGCCTTCCAGCGACTAGATCTGAATCTAAAAACCTTTATGATCAACAATATTAAA
GATGCGATTGCCCGTTGTGAACCAAGAATACGCGTCAAGGAAGTAAAGCTTGAGGAAGAAGATAATATATCCGGCCTGGT
TAATATATACGTAAGCTATATTATAAAGTCTACTAATATGCCAGGGAATTTAGTTTATGCTCACTCTTTTGAATAA