Detailed information of component protein


Summary

Component ID T6CP015301
Component protein type TssI
T6SS ID (Type) T6SS01207 experimental (Type iv)
Strain Candidatus Amoebophilus asiaticus 5a2
Replicon chromosome
Sequence Protein sequence (600 a.a.); Nucleotide sequence (1800 bp)
Reference
-
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) AASI_RS05520
Coordinate (Strand) 1391821..1393620 (+)
NCBI ID WP_012473183.1
RefSeq NC_010830
Uniprot ID B3ET73_AMOA5
KEGG ID aas:Aasi_1080
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_base_V PF04717.14 5.1e-17 377..453
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-599 MSTSATSSDLVNYFIKVGTKEIDKEFPIFSITVNKTINKIPYAKILLHDGDPAEQSFLISNSNIFEIGKVVEIQLGYQSDLTVVFEGVITKHSLKANNYTNPYLEVYCKDIAYQTTLVPKTISFADTTDSGALENILKNYEGDIEQHISSTTIKHEILAQQDTTDWDFINVRAEANGQVVIVDDGTLTTKKPNSSQTPSYTFTYGVDIYGLDLEMDASTQWQQAGGKIWKHDEQACEVVNASTVGEKSFGATSHAKLAGTNKQEPITFIHGGNVTKEEMKSFSTGLLELNRLAKIRGKVTVQGIADLKPDSTIKIDKGADNFEGNAYVSGVHHRLEGGQWFTDITVGLPNERYMRKYNNIAGLPAAGMLCPVYGLQIGIVKELYKQEDPDPNYRIFVNIPIIHQPNEGIWCRVASFYASKGIGAFIMPEKEDEVIIGFVNDDFRSPVIVGSLYSGSKHKTPIQQDPENNIKALVTRSKLEMTFNDKDKAIVFQTPGGRTISISDKSGTIEITNGNANKIILGKQNVEIISNKDIILNAKGSINLQATDSVQIKGNNKVELSGMNVSANATMKASLVGNSSAQVQSSMSTIIKGTIVQIN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9857 - -


  Protein sequence: 600 a.a.    

>T6CP015301 NC_010830:1391821-1393620 [Candidatus Amoebophilus asiaticus 5a2] [TssI]
MSTSATSSDLVNYFIKVGTKEIDKEFPIFSITVNKTINKIPYAKILLHDGDPAEQSFLISNSNIFEIGKVVEIQLGYQSD
LTVVFEGVITKHSLKANNYTNPYLEVYCKDIAYQTTLVPKTISFADTTDSGALENILKNYEGDIEQHISSTTIKHEILAQ
QDTTDWDFINVRAEANGQVVIVDDGTLTTKKPNSSQTPSYTFTYGVDIYGLDLEMDASTQWQQAGGKIWKHDEQACEVVN
ASTVGEKSFGATSHAKLAGTNKQEPITFIHGGNVTKEEMKSFSTGLLELNRLAKIRGKVTVQGIADLKPDSTIKIDKGAD
NFEGNAYVSGVHHRLEGGQWFTDITVGLPNERYMRKYNNIAGLPAAGMLCPVYGLQIGIVKELYKQEDPDPNYRIFVNIP
IIHQPNEGIWCRVASFYASKGIGAFIMPEKEDEVIIGFVNDDFRSPVIVGSLYSGSKHKTPIQQDPENNIKALVTRSKLE
MTFNDKDKAIVFQTPGGRTISISDKSGTIEITNGNANKIILGKQNVEIISNKDIILNAKGSINLQATDSVQIKGNNKVEL
SGMNVSANATMKASLVGNSSAQVQSSMSTIIKGTIVQIN*

  Nucleotide sequence: 1800 bp    

>T6CP015301 NC_010830:1391821-1393620 [Candidatus Amoebophilus asiaticus 5a2] [TssI]
ATGAGCACTTCCGCAACTTCTAGCGACTTAGTTAATTATTTTATTAAAGTTGGCACTAAAGAAATAGATAAAGAATTTCC
TATTTTTTCTATTACTGTAAATAAGACCATTAATAAAATTCCTTATGCAAAAATTCTCTTGCATGATGGAGATCCTGCGG
AACAGTCGTTTCTAATTAGTAATAGTAACATATTTGAAATAGGAAAAGTTGTAGAAATTCAATTAGGCTATCAGTCTGAC
TTAACGGTTGTATTTGAAGGTGTCATTACCAAACATAGTTTAAAAGCTAATAACTACACAAATCCCTATTTAGAAGTATA
TTGTAAAGATATTGCTTACCAAACTACCTTAGTTCCCAAAACAATTAGTTTTGCAGACACTACTGATAGTGGAGCTTTGG
AGAACATATTGAAGAACTACGAAGGCGATATAGAGCAACATATTAGCTCTACAACAATCAAACATGAAATTCTAGCACAA
CAAGATACTACGGACTGGGATTTTATTAACGTGCGTGCAGAAGCTAACGGACAAGTAGTAATAGTAGATGATGGGACCCT
TACTACAAAAAAGCCCAATAGTAGCCAAACACCTAGCTACACATTTACTTATGGGGTTGATATTTATGGGTTAGATTTAG
AAATGGATGCCAGTACACAATGGCAACAAGCAGGCGGCAAAATATGGAAACATGATGAACAAGCATGTGAAGTTGTTAAT
GCAAGTACTGTGGGAGAAAAATCGTTTGGTGCTACTTCACATGCTAAACTAGCAGGTACTAACAAACAGGAACCTATTAC
GTTTATTCATGGAGGTAATGTTACTAAAGAAGAGATGAAAAGCTTCTCCACAGGCCTGTTGGAGCTTAACAGGTTAGCAA
AAATCAGAGGAAAAGTGACTGTGCAAGGAATTGCTGATCTTAAACCAGACAGTACAATCAAAATTGATAAAGGGGCTGAT
AATTTTGAGGGCAATGCTTACGTAAGTGGGGTGCACCACCGGTTAGAAGGAGGACAATGGTTTACAGATATAACTGTGGG
CCTGCCTAACGAACGTTATATGCGTAAATACAACAATATAGCAGGCCTCCCAGCAGCCGGAATGCTTTGTCCTGTCTACG
GATTACAAATAGGTATAGTAAAAGAATTATATAAACAAGAAGACCCAGACCCTAATTACCGAATTTTTGTAAATATCCCT
ATCATTCACCAACCGAACGAGGGTATTTGGTGTAGAGTAGCTTCATTTTACGCTTCTAAAGGCATAGGTGCCTTTATTAT
GCCAGAAAAAGAAGATGAAGTTATCATTGGCTTTGTTAATGATGATTTTAGATCACCGGTCATAGTAGGCTCTCTATATA
GTGGCAGTAAACATAAAACTCCTATTCAGCAAGACCCGGAAAATAATATCAAAGCACTGGTAACCAGAAGCAAGTTAGAG
ATGACTTTTAATGATAAAGATAAAGCAATTGTATTTCAAACTCCAGGAGGAAGGACTATTAGCATTTCTGACAAAAGTGG
TACAATAGAAATTACGAATGGTAACGCGAACAAGATAATTCTAGGTAAGCAGAATGTTGAAATTATTAGTAATAAAGATA
TAATCTTAAATGCCAAAGGAAGTATCAACTTACAGGCAACAGACAGTGTACAGATAAAAGGAAACAATAAAGTAGAACTT
AGTGGCATGAATGTATCTGCCAACGCAACAATGAAAGCTTCATTGGTAGGCAATTCAAGTGCGCAGGTACAATCTAGTAT
GTCTACTATAATAAAAGGAACTATAGTACAAATAAATTAA