Detailed information of component protein


Summary

Component ID T6CP015300
Component protein type T6SSiv-tube2
T6SS ID (Type) T6SS01207 experimental (Type iv)
Strain Candidatus Amoebophilus asiaticus 5a2
Replicon chromosome
Sequence Protein sequence (378 a.a.); Nucleotide sequence (1134 bp)
Reference
-
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) AASI_RS05515
Coordinate (Strand) 1389821..1390954 (+)
NCBI ID WP_012473182.1
RefSeq NC_010830
Uniprot ID B3ET72_AMOA5
KEGG ID aas:Aasi_1079
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
CIS_tube PF19266.1 6.1e-40 3..151
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-169 MSISKLVIKAYSNESFTTQKGEFSASINPANLKITSSVDYERSQGMGSANMALRYNVSPPKELSFRLIFDNTGIFPDSDKSVKDQLEALQDVVYKFQEDINSPYYVRVIWGVIDFKGKLVGLETSYTMFKSDGAPIRAEVDIVVLEDASASKIATAAKAAAKTANTATT
Transmembrane helix 170-192 AVLGAATGAAAGAAAAAVTVAAA
Outside 193-321 SVAVSPNAPPSVAPDATTAGATLTESELADASTPDTAGAKANTSATGTSQAGGEPAAGTNAAATTTNPQNADTKNIEQAPGATAAATPTTVQQVTPKDKLTGVAKNSLGDPNLAKSLGRVNGLDSLRNL
Transmembrane helix 322-344 ASGLSLAVPLTSLGLLAMLLAMA
Inside 345-377 KKYGSKGANYLKSKAKTGKNKAVAAKDKVKSKL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9794 - -


  Protein sequence: 378 a.a.    

>T6CP015300 NC_010830:1389821-1390954 [Candidatus Amoebophilus asiaticus 5a2] [T6SSiv-tube2]
MSISKLVIKAYSNESFTTQKGEFSASINPANLKITSSVDYERSQGMGSANMALRYNVSPPKELSFRLIFDNTGIFPDSDK
SVKDQLEALQDVVYKFQEDINSPYYVRVIWGVIDFKGKLVGLETSYTMFKSDGAPIRAEVDIVVLEDASASKIATAAKAA
AKTANTATTAVLGAATGAAAGAAAAAVTVAAASVAVSPNAPPSVAPDATTAGATLTESELADASTPDTAGAKANTSATGT
SQAGGEPAAGTNAAATTTNPQNADTKNIEQAPGATAAATPTTVQQVTPKDKLTGVAKNSLGDPNLAKSLGRVNGLDSLRN
LASGLSLAVPLTSLGLLAMLLAMAKKYGSKGANYLKSKAKTGKNKAVAAKDKVKSKL*

  Nucleotide sequence: 1134 bp    

>T6CP015300 NC_010830:1389821-1390954 [Candidatus Amoebophilus asiaticus 5a2] [T6SSiv-tube2]
ATGAGTATTAGCAAGCTGGTTATAAAAGCATATAGTAATGAGAGCTTTACTACCCAAAAGGGTGAGTTTTCAGCTTCTAT
CAACCCTGCAAACCTCAAAATTACAAGTAGTGTAGATTATGAGAGGTCTCAAGGTATGGGCTCAGCCAATATGGCGCTTC
GCTATAACGTTTCGCCTCCTAAGGAACTGTCGTTTAGACTCATCTTTGATAATACAGGGATCTTTCCAGACTCAGACAAA
AGTGTAAAAGATCAGCTAGAAGCTTTGCAAGACGTGGTATATAAGTTCCAGGAAGATATTAATTCACCTTATTACGTGCG
GGTTATCTGGGGTGTAATTGATTTTAAAGGTAAATTGGTTGGTTTGGAGACAAGTTATACCATGTTTAAGTCAGATGGTG
CTCCAATCCGAGCAGAAGTAGATATAGTGGTATTAGAAGATGCAAGCGCAAGCAAGATTGCCACAGCTGCAAAAGCAGCT
GCGAAAACGGCTAATACAGCCACTACTGCAGTATTAGGGGCAGCAACTGGTGCAGCGGCTGGGGCCGCAGCCGCTGCCGT
AACCGTAGCAGCAGCGTCGGTTGCTGTAAGCCCTAATGCACCACCTAGTGTAGCGCCTGATGCTACAACTGCTGGAGCTA
CACTCACAGAATCAGAACTTGCTGACGCCAGCACACCAGACACTGCAGGAGCAAAAGCAAACACTAGTGCAACTGGCACT
TCACAAGCAGGAGGAGAACCAGCAGCAGGAACAAATGCCGCAGCCACCACTACCAATCCTCAAAATGCAGATACAAAGAA
TATAGAGCAGGCACCTGGCGCAACTGCAGCTGCTACCCCTACTACGGTACAACAAGTGACACCCAAAGATAAATTAACTG
GTGTTGCTAAAAATTCATTAGGAGATCCAAATCTTGCTAAATCACTAGGCCGTGTAAATGGATTAGACAGCCTTAGGAAC
TTAGCTTCAGGGCTCTCACTAGCTGTTCCTTTAACATCGCTGGGGCTTTTAGCAATGCTATTGGCAATGGCAAAAAAATA
CGGTTCAAAGGGTGCTAATTATTTAAAAAGTAAGGCAAAAACAGGTAAAAACAAGGCTGTTGCAGCTAAAGATAAGGTAA
AGAGTAAACTTTAA