Detailed information of component protein
Summary
| Component ID | T6CP015297 |
| Component protein type | T6SSiv-Afp-like |
| T6SS ID (Type) | T6SS01207  (Type iv) |
| Strain | Candidatus Amoebophilus asiaticus 5a2 |
| Replicon | chromosome |
| Sequence | Protein sequence (149 a.a.); Nucleotide sequence (447 bp) |
| Reference | - |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | AASI_RS05500 |
| Coordinate (Strand) | 1388642..1389088 (+) |
| NCBI ID | WP_012473179.1 |
| RefSeq | NC_010830 |
| Uniprot ID | B3ET69_AMOA5 |
| KEGG ID | aas:Aasi_1076 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-148 | MSTQRFTSFNYSPLTTTCSSAGCTVDSVELPDMQRSHIAKVQTSQQAPLSYHTSERKEIVDFTVTYIPYDNPTGKGLLPFVKTAVAEVLKKGYDPQTATIQIKRGNKTTPEQTTTIDNCIVTAGEILIDVNDYVRIKFYLQGILNAPY |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.7223 | pos: 22-23. SAG-CT. | MSTQRFTSFNYSPLTTTCSSAG |
Protein sequence: 149 a.a.
>T6CP015297 NC_010830:1388642-1389088 [Candidatus Amoebophilus asiaticus 5a2] [T6SSiv-Afp-like ]
MSTQRFTSFNYSPLTTTCSSAGCTVDSVELPDMQRSHIAKVQTSQQAPLSYHTSERKEIVDFTVTYIPYDNPTGKGLLPF
VKTAVAEVLKKGYDPQTATIQIKRGNKTTPEQTTTIDNCIVTAGEILIDVNDYVRIKFYLQGILNAPY*
MSTQRFTSFNYSPLTTTCSSAGCTVDSVELPDMQRSHIAKVQTSQQAPLSYHTSERKEIVDFTVTYIPYDNPTGKGLLPF
VKTAVAEVLKKGYDPQTATIQIKRGNKTTPEQTTTIDNCIVTAGEILIDVNDYVRIKFYLQGILNAPY*
Nucleotide sequence: 447 bp
>T6CP015297 NC_010830:1388642-1389088 [Candidatus Amoebophilus asiaticus 5a2] [T6SSiv-Afp-like ]
ATGAGTACACAAAGGTTTACAAGTTTTAATTATTCACCTTTAACAACCACATGTAGTTCAGCAGGTTGCACAGTAGATTC
AGTAGAACTACCAGACATGCAAAGATCACATATAGCTAAAGTACAGACAAGTCAGCAAGCACCATTATCTTATCATACTT
CTGAAAGAAAAGAAATAGTAGATTTTACGGTTACTTATATCCCATATGACAATCCTACAGGAAAAGGGCTTTTACCTTTC
GTTAAAACAGCTGTAGCGGAAGTACTAAAGAAAGGTTATGACCCTCAAACTGCTACAATACAGATAAAAAGAGGTAATAA
GACCACCCCAGAACAGACAACAACCATCGATAATTGTATTGTTACTGCTGGCGAAATATTAATAGATGTTAATGACTATG
TACGTATCAAATTTTACTTACAAGGTATTTTAAATGCACCTTACTAA
ATGAGTACACAAAGGTTTACAAGTTTTAATTATTCACCTTTAACAACCACATGTAGTTCAGCAGGTTGCACAGTAGATTC
AGTAGAACTACCAGACATGCAAAGATCACATATAGCTAAAGTACAGACAAGTCAGCAAGCACCATTATCTTATCATACTT
CTGAAAGAAAAGAAATAGTAGATTTTACGGTTACTTATATCCCATATGACAATCCTACAGGAAAAGGGCTTTTACCTTTC
GTTAAAACAGCTGTAGCGGAAGTACTAAAGAAAGGTTATGACCCTCAAACTGCTACAATACAGATAAAAAGAGGTAATAA
GACCACCCCAGAACAGACAACAACCATCGATAATTGTATTGTTACTGCTGGCGAAATATTAATAGATGTTAATGACTATG
TACGTATCAAATTTTACTTACAAGGTATTTTAAATGCACCTTACTAA