Detailed information of component protein


Summary

Component ID T6CP015295
Component protein type TssB/TssC
T6SS ID (Type) T6SS01207 experimental (Type iv)
Strain Candidatus Amoebophilus asiaticus 5a2
Replicon chromosome
Sequence Protein sequence (499 a.a.); Nucleotide sequence (1497 bp)
Reference
-
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) AASI_RS05490
Coordinate (Strand) 1386461..1387957 (+)
NCBI ID WP_012473177.1
RefSeq NC_010830
Uniprot ID B3ET67_AMOA5
KEGG ID aas:Aasi_1074
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_sheath_1 PF04984.16 1.7e-08 280..380
Phage_sheath_1C PF17482.4 2.3e-08 386..490
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-498 MPENLKTPGVYIVEKDTGANAVVQVATAVPVFIGFTERAEINGKSFHMKPVHINSLSEFEIFYGKAPVPVFTVKPAEKGGGDLNMNGQMYTLQQSPYSKFYLYNSLKLFFDNGGADCYIISIGQYGKDPQLLAITPDTFKKAIDTLAGEEVPTMLLMPDSLLLDEEDSSYYSVQTYALQHCGKYMDKVALFDIWGSGEELPLGEDKNKYVTRFRENIGLDNLTYGAAYYPWVKTNIISINDIGYENFNLDSLESLINEAHKPILHNIKTATSEKEKKYWDAGLKNASKEYKLLRKTIADRLNVLPAAPAMAGLYTRTDRSRGVWIAPANQNLNSVIEPAIKITHEDQETLNVDAISGKSINAIRAFRGRGSAIVWGARTLAGNNVEWRYINVRRLFILIEQSIKQASFSVVFQPNVSITWAIVKGSIGNFLTNLWRQGALVGNTPSEAFTVSCGLGETMTQEDINEGIMRIKVQAAASRPAEFIVITFEQKMGGQEGS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9854 - -


  Protein sequence: 499 a.a.    

>T6CP015295 NC_010830:1386461-1387957 [Candidatus Amoebophilus asiaticus 5a2] [TssB/TssC]
MPENLKTPGVYIVEKDTGANAVVQVATAVPVFIGFTERAEINGKSFHMKPVHINSLSEFEIFYGKAPVPVFTVKPAEKGG
GDLNMNGQMYTLQQSPYSKFYLYNSLKLFFDNGGADCYIISIGQYGKDPQLLAITPDTFKKAIDTLAGEEVPTMLLMPDS
LLLDEEDSSYYSVQTYALQHCGKYMDKVALFDIWGSGEELPLGEDKNKYVTRFRENIGLDNLTYGAAYYPWVKTNIISIN
DIGYENFNLDSLESLINEAHKPILHNIKTATSEKEKKYWDAGLKNASKEYKLLRKTIADRLNVLPAAPAMAGLYTRTDRS
RGVWIAPANQNLNSVIEPAIKITHEDQETLNVDAISGKSINAIRAFRGRGSAIVWGARTLAGNNVEWRYINVRRLFILIE
QSIKQASFSVVFQPNVSITWAIVKGSIGNFLTNLWRQGALVGNTPSEAFTVSCGLGETMTQEDINEGIMRIKVQAAASRP
AEFIVITFEQKMGGQEGS*

  Nucleotide sequence: 1497 bp    

>T6CP015295 NC_010830:1386461-1387957 [Candidatus Amoebophilus asiaticus 5a2] [TssB/TssC]
ATGCCAGAAAATTTAAAAACTCCCGGCGTATATATCGTCGAGAAGGACACCGGTGCCAATGCTGTGGTTCAGGTGGCAAC
TGCAGTTCCCGTTTTCATAGGATTTACCGAGCGGGCAGAAATCAATGGAAAATCTTTCCATATGAAGCCGGTGCATATTA
ACTCTTTATCTGAGTTTGAAATATTCTATGGAAAAGCACCTGTGCCTGTCTTTACCGTTAAACCTGCAGAAAAAGGAGGT
GGAGATCTTAATATGAATGGACAAATGTATACCTTACAACAAAGTCCTTATTCAAAATTTTACCTATACAATAGTTTAAA
ATTATTTTTTGATAATGGTGGTGCAGATTGCTACATCATATCTATTGGACAATATGGTAAAGATCCACAACTGCTAGCAA
TTACCCCTGATACATTCAAAAAAGCAATAGATACCTTAGCAGGCGAAGAAGTACCTACTATGTTGCTTATGCCCGACTCT
CTGCTACTAGATGAAGAAGATTCTTCTTATTATTCTGTACAAACATATGCTTTGCAACATTGTGGCAAATATATGGATAA
AGTAGCGCTATTTGATATCTGGGGAAGTGGAGAAGAGCTTCCATTAGGAGAAGACAAAAATAAATATGTAACTCGATTTA
GAGAAAATATAGGCTTAGACAACCTAACCTATGGTGCAGCGTACTACCCTTGGGTTAAAACCAATATCATATCAATCAAC
GATATTGGATATGAGAACTTTAATTTAGATTCTTTAGAATCTCTTATTAATGAAGCACACAAACCTATCCTGCACAATAT
CAAAACTGCTACTAGCGAAAAGGAGAAAAAATATTGGGATGCAGGACTTAAAAATGCTAGTAAAGAATATAAGCTTCTAC
GTAAAACTATAGCAGACAGACTTAATGTATTGCCAGCAGCACCAGCTATGGCAGGGTTGTACACACGTACTGATAGAAGT
AGAGGCGTATGGATAGCACCAGCTAACCAAAACCTAAATTCTGTTATTGAGCCTGCTATTAAGATTACGCATGAAGATCA
AGAAACTCTTAACGTAGATGCTATAAGCGGAAAATCTATTAATGCTATCCGTGCATTTAGAGGAAGAGGATCTGCTATTG
TTTGGGGGGCAAGAACGTTGGCAGGCAACAATGTAGAATGGCGCTATATTAACGTAAGGAGATTATTTATACTTATTGAA
CAGTCTATCAAACAAGCATCCTTCTCTGTTGTATTCCAACCTAACGTATCCATAACCTGGGCTATAGTAAAAGGAAGTAT
TGGTAACTTCTTAACCAACTTGTGGAGACAAGGTGCTTTAGTAGGAAACACTCCTTCTGAAGCCTTTACAGTAAGCTGTG
GACTTGGTGAAACTATGACTCAAGAAGACATTAATGAAGGTATCATGCGAATAAAAGTTCAGGCAGCAGCTTCTAGACCA
GCAGAGTTTATCGTCATTACATTTGAGCAAAAGATGGGCGGACAAGAAGGAAGTTAA