Detailed information of component protein
Summary
| Component ID | T6CP015294 |
| Component protein type | T6SSiv-Afp-like |
| T6SS ID (Type) | T6SS01207  (Type iv) |
| Strain | Candidatus Amoebophilus asiaticus 5a2 |
| Replicon | chromosome |
| Sequence | Protein sequence (283 a.a.); Nucleotide sequence (849 bp) |
| Reference | - |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | AASI_RS05485 |
| Coordinate (Strand) | 1385590..1386438 (+) |
| NCBI ID | WP_012473176.1 |
| RefSeq | NC_010830 |
| Uniprot ID | B3ET66_AMOA5 |
| KEGG ID | aas:Aasi_1073 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-282 | MVFALKLFRINIYNSYYSNQVDNNFHLVADETTQQLMDYHRMRLNEYEGGYEIIWLTNKFDEPLQVFQDKIASSTLTFYFILRNPYTINFSLLKPEQGYIYYFSNTNNSDNLHKKAYVAEEDKVPFSTSIHSIGKPFPNAFGIIHVQLANLWEKKPSIEKFPVQYNIKIKARETIWRYHIIDVQERIKSPMKVVIDEDDSYFIYKGTPENKHIHVYESKKALTLSDKPSQKFSLRMISKKTTKKAANTEETLLERLPLPDTHLLENNVEKGGIFYSNIVVYV |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9822 | - | - |
Protein sequence: 283 a.a.
>T6CP015294 NC_010830:1385590-1386438 [Candidatus Amoebophilus asiaticus 5a2] [T6SSiv-Afp-like ]
MVFALKLFRINIYNSYYSNQVDNNFHLVADETTQQLMDYHRMRLNEYEGGYEIIWLTNKFDEPLQVFQDKIASSTLTFYF
ILRNPYTINFSLLKPEQGYIYYFSNTNNSDNLHKKAYVAEEDKVPFSTSIHSIGKPFPNAFGIIHVQLANLWEKKPSIEK
FPVQYNIKIKARETIWRYHIIDVQERIKSPMKVVIDEDDSYFIYKGTPENKHIHVYESKKALTLSDKPSQKFSLRMISKK
TTKKAANTEETLLERLPLPDTHLLENNVEKGGIFYSNIVVYV*
MVFALKLFRINIYNSYYSNQVDNNFHLVADETTQQLMDYHRMRLNEYEGGYEIIWLTNKFDEPLQVFQDKIASSTLTFYF
ILRNPYTINFSLLKPEQGYIYYFSNTNNSDNLHKKAYVAEEDKVPFSTSIHSIGKPFPNAFGIIHVQLANLWEKKPSIEK
FPVQYNIKIKARETIWRYHIIDVQERIKSPMKVVIDEDDSYFIYKGTPENKHIHVYESKKALTLSDKPSQKFSLRMISKK
TTKKAANTEETLLERLPLPDTHLLENNVEKGGIFYSNIVVYV*
Nucleotide sequence: 849 bp
>T6CP015294 NC_010830:1385590-1386438 [Candidatus Amoebophilus asiaticus 5a2] [T6SSiv-Afp-like ]
ATGGTTTTTGCTTTAAAATTGTTTAGAATTAATATTTACAATAGTTACTACAGTAACCAAGTAGATAATAACTTTCATTT
GGTAGCTGATGAAACAACCCAACAGCTGATGGATTACCATCGTATGCGGCTTAACGAATATGAAGGAGGCTATGAAATAA
TTTGGCTAACAAATAAGTTTGATGAACCTCTTCAAGTTTTTCAAGATAAAATAGCAAGCTCCACTCTAACCTTTTATTTC
ATATTACGCAATCCATATACAATCAACTTTTCCTTACTAAAACCAGAACAAGGATATATTTATTACTTCAGCAACACAAA
TAACTCCGATAACTTACATAAAAAAGCTTATGTTGCTGAAGAAGATAAAGTACCTTTTAGTACTTCTATACATAGCATAG
GAAAACCATTTCCAAATGCATTTGGTATCATTCATGTTCAGTTAGCTAACCTATGGGAAAAAAAGCCATCAATAGAGAAG
TTTCCTGTGCAATACAACATTAAAATTAAAGCTAGGGAAACTATTTGGCGCTACCATATAATAGATGTACAAGAGCGCAT
AAAAAGTCCTATGAAAGTTGTAATTGATGAAGATGATTCTTATTTTATATATAAAGGAACTCCTGAAAATAAGCATATCC
ATGTATATGAGTCTAAAAAAGCGCTTACACTAAGCGACAAACCCAGTCAAAAGTTTTCTTTAAGAATGATCTCAAAAAAA
ACAACAAAAAAAGCAGCTAATACAGAAGAAACACTGTTAGAAAGATTACCCCTTCCAGACACTCATCTGTTAGAAAACAA
TGTAGAAAAAGGAGGCATATTTTATAGTAATATCGTTGTCTATGTTTAA
ATGGTTTTTGCTTTAAAATTGTTTAGAATTAATATTTACAATAGTTACTACAGTAACCAAGTAGATAATAACTTTCATTT
GGTAGCTGATGAAACAACCCAACAGCTGATGGATTACCATCGTATGCGGCTTAACGAATATGAAGGAGGCTATGAAATAA
TTTGGCTAACAAATAAGTTTGATGAACCTCTTCAAGTTTTTCAAGATAAAATAGCAAGCTCCACTCTAACCTTTTATTTC
ATATTACGCAATCCATATACAATCAACTTTTCCTTACTAAAACCAGAACAAGGATATATTTATTACTTCAGCAACACAAA
TAACTCCGATAACTTACATAAAAAAGCTTATGTTGCTGAAGAAGATAAAGTACCTTTTAGTACTTCTATACATAGCATAG
GAAAACCATTTCCAAATGCATTTGGTATCATTCATGTTCAGTTAGCTAACCTATGGGAAAAAAAGCCATCAATAGAGAAG
TTTCCTGTGCAATACAACATTAAAATTAAAGCTAGGGAAACTATTTGGCGCTACCATATAATAGATGTACAAGAGCGCAT
AAAAAGTCCTATGAAAGTTGTAATTGATGAAGATGATTCTTATTTTATATATAAAGGAACTCCTGAAAATAAGCATATCC
ATGTATATGAGTCTAAAAAAGCGCTTACACTAAGCGACAAACCCAGTCAAAAGTTTTCTTTAAGAATGATCTCAAAAAAA
ACAACAAAAAAAGCAGCTAATACAGAAGAAACACTGTTAGAAAGATTACCCCTTCCAGACACTCATCTGTTAGAAAACAA
TGTAGAAAAAGGAGGCATATTTTATAGTAATATCGTTGTCTATGTTTAA