Detailed information of component protein
Summary
| Component ID | T6CP015289 |
| Component protein type | T6SSiv-spike |
| T6SS ID (Type) | T6SS01206  (Type iv) |
| Strain | Candidatus Amoebophilus asiaticus 5a2 |
| Replicon | chromosome |
| Sequence | Protein sequence (1119 a.a.); Nucleotide sequence (3357 bp) |
| Reference | - |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | |
| Coordinate (Strand) | 722849..726205 (+) |
| NCBI ID | WP_187146293.1 |
| RefSeq | NC_010830 |
| Uniprot ID | UPI00164F1C49 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-4 | MLKD |
| Transmembrane helix | 5-27 | YLDIILFTTFLLVNLIIGLIAGR |
| Inside | 28-46 | RVRSLRDFSIGNKDFTTAT |
| Transmembrane helix | 47-69 | VTSTIVATWFGGGFIFYGLQNAY |
| Outside | 70-72 | TSG |
| Transmembrane helix | 73-95 | LQYIIPLLGSSLCLLFTGQVLAV |
| Inside | 96-115 | RMGEFLNNLSVAEAMGNLFS |
| Transmembrane helix | 116-138 | RLVRIITAISGILGGIGYIAIQF |
| Outside | 139-147 | QVIAKMLNF |
| Transmembrane helix | 148-170 | LLGFEGPWVTVAAASIVIIYSAF |
| Inside | 171-176 | GGIRSV |
| Transmembrane helix | 177-199 | IITDLLQFIVFSIFIPILALVVW |
| Outside | 200-230 | NHLKKPGQVAHTLVTNPVFNFKEMLGWNPKF |
| Transmembrane helix | 231-253 | LSALGLVLYFAIPGITPAIFQRV |
| Inside | 254-265 | TISKDLRQVKDS |
| Transmembrane helix | 266-288 | FTYAAASSLLMVASLAWIAILLL |
| Outside | 289-302 | SDNPNLESGSLVNY |
| Transmembrane helix | 303-325 | IITTYAYPGLKGLIAIGITAMAM |
| Inside | 326-354 | STADSYLNSSAVLAVNDILKPLQLYWKDS |
| Transmembrane helix | 355-377 | IKIVRVFSFVLGIFALLLALRST |
| Outside | 378-381 | DLLQ |
| Transmembrane helix | 382-404 | LMLLSGSFYMPIVTVPLLLAIFG |
| Inside | 405-410 | FRSSSR |
| Transmembrane helix | 411-430 | SVLIGMSAGCITVILWNKFL |
| Outside | 431-433 | THT |
| Transmembrane helix | 434-456 | GMQSLIPGMIANLVFYVGSHYIL |
| Inside | 457-503 | RQQGGWIGIRDREPLLAARQGRREAWQKFIYKVRHPQIYTYLQKNLP |
| Transmembrane helix | 504-526 | AYEVVYTLFAIYVIGATYASFYT |
| Outside | 527-540 | IPESTVANYQKLYD |
| Transmembrane helix | 541-560 | IAAHSVLVMTAGFLTYPAWP |
| Inside | 561-566 | PTFKAK |
| Transmembrane helix | 567-589 | WFITFAWPAGILYVLFIVGTILV |
| Outside | 590-598 | LMSGFHEVQ |
| Transmembrane helix | 599-633 | VMIFLINLIMTAFLLSWPLMLFVSLVGIIIGCLVF |
| Inside | 634-653 | YMYCGDLYACTSSTGSGQFK |
| Transmembrane helix | 654-671 | VIYGILLFSSFLIALFRF |
| Outside | 672-1118 | KQSQTRLENKNVYLENIYKQLKDELSEILGYKETLVKELKEDELALFGTTAAAYMQQAIYRITDYVRLEVSQIKLEDLIVEIKDILKLKDFDGQSPQLSSKKYTKEESIYADADKIKQVLADSICYIHKHNLSNKTIVIALEDAMLGHSVEHMKDYTRKLKAIKITITTQELLSNTSNRVYMFDQAPSISRMAGDGDRKALLNNARIIDAHYGYAELDKEDTHIYVLPINVREVRGKVMELLRAPAVASEEEVKHPLAIQLEEELLNKTKTAKLDTNVIEKSLNTIKRYHAGVKRKSGEPFFTHPIAVALILLDYCKDQDAVIAALLHDTVEDTSLSITHIKAMFGEKVAFIVGKVTNLEDKIRRLSLEEHENIKRLINYEDERAAFVKLADRLHNMRTIEGHLSLSKQKHIANETLLFFVPLANQLGVDHVAQELEKLSLEVLAKK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9795 | - | - |
Protein sequence: 1119 a.a.
Nucleotide sequence: 3357 bp