Detailed information of component protein


Summary

Component ID T6CP015289
Component protein type T6SSiv-spike
T6SS ID (Type) T6SS01206 experimental (Type iv)
Strain Candidatus Amoebophilus asiaticus 5a2
Replicon chromosome
Sequence Protein sequence (1119 a.a.); Nucleotide sequence (3357 bp)
Reference
-
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) AASI_RS02890
Coordinate (Strand) 722849..726205 (+)
NCBI ID WP_187146293.1
RefSeq NC_010830
Uniprot ID UPI00164F1C49
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
HD_4 PF13328.8 2.6e-28 957..1100
SSF PF00474.19 1.1e-09 37..419
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-4 MLKD
Transmembrane helix 5-27 YLDIILFTTFLLVNLIIGLIAGR
Inside 28-46 RVRSLRDFSIGNKDFTTAT
Transmembrane helix 47-69 VTSTIVATWFGGGFIFYGLQNAY
Outside 70-72 TSG
Transmembrane helix 73-95 LQYIIPLLGSSLCLLFTGQVLAV
Inside 96-115 RMGEFLNNLSVAEAMGNLFS
Transmembrane helix 116-138 RLVRIITAISGILGGIGYIAIQF
Outside 139-147 QVIAKMLNF
Transmembrane helix 148-170 LLGFEGPWVTVAAASIVIIYSAF
Inside 171-176 GGIRSV
Transmembrane helix 177-199 IITDLLQFIVFSIFIPILALVVW
Outside 200-230 NHLKKPGQVAHTLVTNPVFNFKEMLGWNPKF
Transmembrane helix 231-253 LSALGLVLYFAIPGITPAIFQRV
Inside 254-265 TISKDLRQVKDS
Transmembrane helix 266-288 FTYAAASSLLMVASLAWIAILLL
Outside 289-302 SDNPNLESGSLVNY
Transmembrane helix 303-325 IITTYAYPGLKGLIAIGITAMAM
Inside 326-354 STADSYLNSSAVLAVNDILKPLQLYWKDS
Transmembrane helix 355-377 IKIVRVFSFVLGIFALLLALRST
Outside 378-381 DLLQ
Transmembrane helix 382-404 LMLLSGSFYMPIVTVPLLLAIFG
Inside 405-410 FRSSSR
Transmembrane helix 411-430 SVLIGMSAGCITVILWNKFL
Outside 431-433 THT
Transmembrane helix 434-456 GMQSLIPGMIANLVFYVGSHYIL
Inside 457-503 RQQGGWIGIRDREPLLAARQGRREAWQKFIYKVRHPQIYTYLQKNLP
Transmembrane helix 504-526 AYEVVYTLFAIYVIGATYASFYT
Outside 527-540 IPESTVANYQKLYD
Transmembrane helix 541-560 IAAHSVLVMTAGFLTYPAWP
Inside 561-566 PTFKAK
Transmembrane helix 567-589 WFITFAWPAGILYVLFIVGTILV
Outside 590-598 LMSGFHEVQ
Transmembrane helix 599-633 VMIFLINLIMTAFLLSWPLMLFVSLVGIIIGCLVF
Inside 634-653 YMYCGDLYACTSSTGSGQFK
Transmembrane helix 654-671 VIYGILLFSSFLIALFRF
Outside 672-1118 KQSQTRLENKNVYLENIYKQLKDELSEILGYKETLVKELKEDELALFGTTAAAYMQQAIYRITDYVRLEVSQIKLEDLIVEIKDILKLKDFDGQSPQLSSKKYTKEESIYADADKIKQVLADSICYIHKHNLSNKTIVIALEDAMLGHSVEHMKDYTRKLKAIKITITTQELLSNTSNRVYMFDQAPSISRMAGDGDRKALLNNARIIDAHYGYAELDKEDTHIYVLPINVREVRGKVMELLRAPAVASEEEVKHPLAIQLEEELLNKTKTAKLDTNVIEKSLNTIKRYHAGVKRKSGEPFFTHPIAVALILLDYCKDQDAVIAALLHDTVEDTSLSITHIKAMFGEKVAFIVGKVTNLEDKIRRLSLEEHENIKRLINYEDERAAFVKLADRLHNMRTIEGHLSLSKQKHIANETLLFFVPLANQLGVDHVAQELEKLSLEVLAKK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9795 - -


  Protein sequence: 1119 a.a.    

>T6CP015289 NC_010830:722849-726205 [Candidatus Amoebophilus asiaticus 5a2] [T6SSiv-spike]
MLKDYLDIILFTTFLLVNLIIGLIAGRRVRSLRDFSIGNKDFTTATVTSTIVATWFGGGFIFYGLQNAYTSGLQYIIPLL
GSSLCLLFTGQVLAVRMGEFLNNLSVAEAMGNLFSRLVRIITAISGILGGIGYIAIQFQVIAKMLNFLLGFEGPWVTVAA
ASIVIIYSAFGGIRSVIITDLLQFIVFSIFIPILALVVWNHLKKPGQVAHTLVTNPVFNFKEMLGWNPKFLSALGLVLYF
AIPGITPAIFQRVTISKDLRQVKDSFTYAAASSLLMVASLAWIAILLLSDNPNLESGSLVNYIITTYAYPGLKGLIAIGI
TAMAMSTADSYLNSSAVLAVNDILKPLQLYWKDSIKIVRVFSFVLGIFALLLALRSTDLLQLMLLSGSFYMPIVTVPLLL
AIFGFRSSSRSVLIGMSAGCITVILWNKFLTHTGMQSLIPGMIANLVFYVGSHYILRQQGGWIGIRDREPLLAARQGRRE
AWQKFIYKVRHPQIYTYLQKNLPAYEVVYTLFAIYVIGATYASFYTIPESTVANYQKLYDIAAHSVLVMTAGFLTYPAWP
PTFKAKWFITFAWPAGILYVLFIVGTILVLMSGFHEVQVMIFLINLIMTAFLLSWPLMLFVSLVGIIIGCLVFYMYCGDL
YACTSSTGSGQFKVIYGILLFSSFLIALFRFKQSQTRLENKNVYLENIYKQLKDELSEILGYKETLVKELKEDELALFGT
TAAAYMQQAIYRITDYVRLEVSQIKLEDLIVEIKDILKLKDFDGQSPQLSSKKYTKEESIYADADKIKQVLADSICYIHK
HNLSNKTIVIALEDAMLGHSVEHMKDYTRKLKAIKITITTQELLSNTSNRVYMFDQAPSISRMAGDGDRKALLNNARIID
AHYGYAELDKEDTHIYVLPINVREVRGKVMELLRAPAVASEEEVKHPLAIQLEEELLNKTKTAKLDTNVIEKSLNTIKRY
HAGVKRKSGEPFFTHPIAVALILLDYCKDQDAVIAALLHDTVEDTSLSITHIKAMFGEKVAFIVGKVTNLEDKIRRLSLE
EHENIKRLINYEDERAAFVKLADRLHNMRTIEGHLSLSKQKHIANETLLFFVPLANQLGVDHVAQELEKLSLEVLAKK*

  Nucleotide sequence: 3357 bp    

>T6CP015289 NC_010830:722849-726205 [Candidatus Amoebophilus asiaticus 5a2] [T6SSiv-spike]
ATGCTAAAAGATTACTTAGATATCATACTATTCACTACATTTTTACTTGTTAACTTAATAATAGGCCTGATCGCTGGTCG
ACGTGTAAGAAGCTTGCGAGATTTCTCCATAGGTAATAAAGATTTTACTACAGCTACGGTAACTTCTACCATTGTAGCTA
CCTGGTTTGGAGGTGGATTTATATTTTATGGATTACAAAATGCTTATACAAGTGGACTGCAATATATTATACCACTTCTG
GGATCAAGTTTATGTTTGCTATTTACTGGTCAAGTACTTGCTGTACGTATGGGCGAATTTTTAAATAATCTTTCTGTTGC
CGAGGCAATGGGAAATCTATTTAGCCGATTGGTACGTATAATTACTGCTATTAGCGGAATACTTGGTGGAATTGGTTATA
TAGCCATTCAGTTTCAGGTGATTGCTAAAATGTTAAACTTCCTCTTAGGATTTGAGGGGCCTTGGGTTACTGTTGCAGCA
GCTTCTATCGTCATTATTTATTCAGCTTTCGGTGGTATCCGATCTGTTATTATTACAGATTTATTGCAGTTTATTGTCTT
TAGTATTTTTATCCCTATTCTTGCCTTGGTGGTTTGGAACCACCTAAAAAAACCTGGACAAGTAGCACATACCTTAGTTA
CCAATCCGGTATTTAACTTCAAGGAAATGCTAGGTTGGAACCCTAAATTTTTATCTGCACTAGGCTTAGTGCTTTATTTT
GCTATTCCGGGCATAACTCCTGCTATATTCCAGCGCGTAACTATATCTAAAGATCTTAGACAAGTTAAAGATTCTTTTAC
CTATGCAGCTGCAAGTTCGTTACTGATGGTTGCTTCATTAGCTTGGATTGCTATTTTATTACTGTCAGATAATCCTAATC
TAGAGTCTGGTAGTCTTGTAAACTATATTATTACAACCTATGCTTATCCAGGACTGAAAGGACTTATTGCTATTGGTATT
ACAGCCATGGCTATGTCTACAGCAGACTCTTATCTTAATTCATCTGCTGTGTTAGCAGTTAATGATATTCTGAAACCACT
GCAGCTTTATTGGAAAGATTCTATTAAGATCGTTAGAGTTTTTTCCTTTGTTTTAGGAATTTTTGCATTGTTATTAGCGC
TTCGCAGTACAGACTTACTACAATTGATGCTACTGTCTGGCAGCTTTTATATGCCTATTGTTACTGTACCACTCTTATTA
GCCATTTTTGGTTTTCGAAGTAGTAGCAGATCAGTTCTTATAGGAATGTCAGCAGGCTGTATAACTGTGATATTATGGAA
TAAGTTTTTAACTCATACAGGCATGCAAAGTCTTATCCCTGGTATGATCGCTAACTTAGTATTCTATGTAGGCAGTCATT
ATATACTTAGGCAACAAGGGGGCTGGATAGGCATACGAGATAGAGAACCCCTTTTGGCAGCTCGGCAAGGCCGTAGAGAG
GCATGGCAGAAATTTATTTATAAGGTTAGACATCCACAAATCTATACTTACTTACAAAAAAATCTGCCGGCTTATGAAGT
TGTTTATACGCTTTTTGCCATTTATGTGATTGGTGCCACGTATGCTTCTTTTTACACCATACCAGAGTCAACAGTTGCCA
ATTATCAAAAGCTCTATGATATTGCTGCACATTCTGTTTTAGTTATGACGGCTGGGTTTCTTACCTATCCTGCTTGGCCC
CCTACTTTTAAAGCCAAATGGTTCATCACCTTTGCTTGGCCAGCGGGTATCCTGTATGTACTCTTTATAGTAGGGACTAT
CTTAGTACTTATGAGTGGCTTTCATGAAGTACAAGTCATGATTTTTCTAATTAATCTAATCATGACTGCATTTTTACTTT
CCTGGCCCCTAATGTTATTTGTTTCTTTGGTAGGTATAATTATTGGCTGCCTAGTTTTTTACATGTATTGTGGGGATTTA
TATGCTTGTACCAGTTCAACTGGTTCAGGGCAATTTAAAGTTATTTATGGCATTCTTTTGTTTAGTAGCTTTCTTATTGC
TCTATTCAGATTTAAACAAAGCCAAACAAGGCTCGAGAATAAGAATGTTTATTTAGAAAATATATATAAACAATTAAAAG
ATGAACTATCTGAAATTTTAGGCTATAAAGAGACGCTTGTTAAAGAACTAAAAGAAGATGAGTTGGCTTTGTTTGGAACA
ACCGCAGCTGCTTACATGCAACAAGCCATTTATCGTATTACCGATTATGTAAGATTAGAAGTAAGCCAGATCAAACTAGA
AGATCTTATAGTAGAAATTAAAGATATACTCAAGTTAAAAGACTTTGATGGACAGTCCCCTCAACTAAGCAGTAAAAAAT
ATACTAAAGAGGAGTCAATCTATGCGGATGCTGATAAGATCAAACAAGTGCTTGCTGACAGTATCTGTTATATCCATAAA
CATAATCTATCCAATAAAACTATTGTTATTGCACTTGAAGATGCCATGCTAGGCCATAGTGTAGAACATATGAAAGATTA
TACACGAAAACTAAAGGCTATTAAAATTACCATTACAACCCAGGAACTATTATCTAATACCTCTAATCGTGTATATATGT
TTGATCAAGCACCTTCTATTAGTCGGATGGCTGGGGATGGAGATAGAAAAGCTTTGTTAAATAATGCACGTATTATCGAT
GCTCATTATGGTTATGCAGAGCTAGATAAGGAAGATACCCATATATATGTTCTTCCTATCAATGTACGTGAAGTAAGGGG
AAAGGTTATGGAGCTTTTAAGGGCGCCTGCAGTGGCCAGTGAGGAAGAAGTAAAGCATCCTTTAGCCATACAGCTGGAGG
AAGAGCTACTTAATAAAACAAAAACTGCTAAACTTGATACAAATGTTATAGAAAAATCACTTAATACCATTAAGAGGTAT
CATGCAGGAGTTAAAAGAAAATCAGGAGAACCTTTCTTCACTCACCCAATAGCTGTAGCATTGATCTTGCTAGATTATTG
CAAAGATCAGGATGCCGTAATAGCAGCACTACTACATGATACAGTGGAAGATACTAGCTTATCTATAACCCATATTAAGG
CTATGTTTGGAGAAAAAGTAGCTTTTATAGTAGGAAAGGTAACCAACCTAGAGGATAAAATAAGAAGGCTAAGCCTTGAG
GAACATGAAAATATTAAAAGGTTAATTAACTATGAAGATGAGCGGGCGGCTTTTGTAAAATTAGCTGACAGGCTTCATAA
CATGCGTACCATTGAAGGACATTTGTCCTTATCTAAACAAAAGCATATAGCCAATGAGACCTTGCTTTTCTTTGTACCAC
TTGCCAACCAATTAGGGGTAGACCATGTGGCACAGGAATTAGAAAAACTTAGTTTGGAAGTATTGGCTAAGAAATGA