Detailed information of component protein


Summary

Component ID T6CP015230
Component protein type TssI
T6SS ID (Type) T6SS01200 experimental (Type i2)
Strain Escherichia coli KTE165
Replicon chromosome
Sequence Protein sequence (853 a.a.); Nucleotide sequence (2559 bp)
Reference
-
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) A31K_RS20835
Coordinate (Strand) 190090..192648 (+)
NCBI ID 486400243
RefSeq NZ_KB733141
Uniprot ID UPI0002A40196
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 6.2e-58 30..345
DUF2345 PF10106.11 3.8e-46 602..748
T6SS_Vgr PF13296.8 2.9e-36 484..585
Phage_base_V PF04717.14 1.8e-09 397..463
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-852 MNVQFFDHAHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQFTSTDKAIAPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARYEVRLEPRMALLARSRQNAIYQNLTVPQIVEKILRERHQMRGQDFVFNLKSEYPSREQVMQYGEDDLTFVSRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGLFDGETEAVWGLNTAYSVVEKNVTTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYADNFLQKGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIRVQGDDAPAVFRKGVLITGVTASAARDCSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKNDIYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEYGGKSQLNLGHLVDAGKQQRGEGFELRTDLWGAVRAKKGIFISADAQDKAQGQVLDMQAAIAQLENALSIAKNLSQAAEVAKAHSADLSEHESLNKALSELAEAGIVLSAPKGVGVVSPRAVRLGSGESSVGLMAGTNVDVSAMKKITLTAGDSVGVFARKDGMKLYANQGKVEVEAQNGGMRLTSRKDMEINSTENRVEITAKEELVLSCGGAYIRLKGGKIELGSPGNILLKSVNVQKMSKARLSPAMPVLPGEGNYDLSLELRDWEGAPISGAKYKITFESGSVLSGILDEQGYTLHTNVPPESASVEYDLPDPLPDAPWDPYASLLAAVDAEFSTNGQGAK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9928 - -


  Protein sequence: 853 a.a.    

>T6CP015230 NZ_KB733141:190090-192648 [Escherichia coli KTE165] [TssI]
MNVQFFDHAHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQFTSTDKAIAPESVLMQDGAFSLTAPPVQGMPVQTALRT
LHGVITGFKHLSSSQDEARYEVRLEPRMALLARSRQNAIYQNLTVPQIVEKILRERHQMRGQDFVFNLKSEYPSREQVMQ
YGEDDLTFVSRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGLFDGETEAVWGLNTAYSVVEKNVTT
RDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYADNFLQKGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGL
EIRVQGDDAPAVFRKGVLITGVTASAARDCSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKNDIYAHIDKD
GRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKR
NVLRTPANNKIRLDDERGKEHIKVSTEYGGKSQLNLGHLVDAGKQQRGEGFELRTDLWGAVRAKKGIFISADAQDKAQGQ
VLDMQAAIAQLENALSIAKNLSQAAEVAKAHSADLSEHESLNKALSELAEAGIVLSAPKGVGVVSPRAVRLGSGESSVGL
MAGTNVDVSAMKKITLTAGDSVGVFARKDGMKLYANQGKVEVEAQNGGMRLTSRKDMEINSTENRVEITAKEELVLSCGG
AYIRLKGGKIELGSPGNILLKSVNVQKMSKARLSPAMPVLPGEGNYDLSLELRDWEGAPISGAKYKITFESGSVLSGILD
EQGYTLHTNVPPESASVEYDLPDPLPDAPWDPYASLLAAVDAEFSTNGQGAK*

  Nucleotide sequence: 2559 bp    

>T6CP015230 NZ_KB733141:190090-192648 [Escherichia coli KTE165] [TssI]
ATGAACGTACAGTTCTTCGACCACGCACACCACAAGCTCAAAATTCGCGGCCTGCAAAGCCCGGTGGATGTGCTGACCTT
TGAAGGCCGCGAGCAACTGAGTACCCCGTTCCGCTACGATATCCAGTTTACCAGTACCGATAAAGCCATCGCGCCGGAGT
CGGTGCTGATGCAGGATGGCGCATTCAGCCTGACGGCACCGCCGGTACAGGGGATGCCGGTACAGACTGCCCTGCGCACC
CTGCACGGGGTGATAACCGGCTTTAAGCATCTCTCCTCGTCACAGGACGAAGCCCGCTATGAAGTGCGTCTTGAACCCCG
TATGGCGCTGCTGGCCCGCAGTCGCCAGAACGCCATCTACCAGAATCTGACGGTGCCGCAAATCGTCGAAAAAATCCTGC
GCGAGCGGCACCAGATGCGCGGTCAGGACTTCGTGTTTAACCTCAAAAGCGAATACCCGTCCCGCGAACAGGTGATGCAG
TACGGTGAGGACGACCTGACGTTTGTCTCACGCCTGCTGTCGGAAGTGGGTATCTGGTTCCGCTTTGCCACCGACGCCCG
GCTGAAAATCGAAGTTGTCGAGTTTTATGACGACCAGAGCGGCTATGAGCGCGGGCTGACGCTGCCGCTTCGCCATCCTT
CAGGGCTGTTTGATGGTGAAACGGAAGCCGTATGGGGGCTGAACACGGCATACAGCGTGGTGGAGAAAAACGTCACCACG
CGGGACTACAACTACCGCACCGCCACAGCAGAGATGATGACAGAGCAGCATGACGCCACCGGGGGCGATAACACCACCTA
CGGCGAAGCCTACCACTATGCTGACAACTTCCTGCAAAAAGGCGATAAAGAGGCTGCGGAAAGCGGCGCGTTTTATGCCC
GTATCCGCCACGAACGCTACCTGAATGAACAGGCCATTCTCAAAGGCCAGAGCACCAGTTCACTGCTGATGCCGGGGCTG
GAAATCAGGGTTCAGGGCGACGACGCGCCAGCAGTGTTCCGCAAGGGTGTGCTGATTACCGGCGTGACGGCCAGCGCGGC
GCGTGACTGCAGCTATGAACTGACGTTTACTGCCATCCCGTACTCAGAACGCTACGGCTACCGCCCGGCGCTTATCCCCC
GCCCGGTAATGGCCGGCACGCTCCCGGCGCGGGTCACCAGCACGGTGAAAAACGATATCTATGCCCATATCGACAAGGAC
GGGCGCTACCGGGTCAACCTCGACTTTGACCGCGATACGTGGAAACCGGGTTATGAAAGCCTGTGGGTGCGCCAGTCGCG
ACCCTACGCCGGAGACACCTACGGCCTGCACCTGCCGCTGCTGGCGGGAACGGAAGTCAGCATCGCCTTTGAGGAAGGCA
ACCCGGACCGACCTTATATTGCAGGCGTAAAACACGACTCGGCACACACTGACCATGTGACCATTCAGAACTACAAGCGC
AACGTGCTACGCACCCCGGCGAACAACAAAATCCGGCTGGATGACGAGCGCGGCAAGGAGCATATCAAGGTCAGTACCGA
GTACGGCGGCAAGAGCCAGCTAAACCTCGGCCATCTGGTGGATGCCGGAAAACAGCAGCGCGGGGAAGGTTTTGAACTGC
GTACCGATCTGTGGGGTGCAGTACGGGCAAAGAAAGGCATCTTTATCAGCGCCGACGCGCAGGACAAGGCGCAGGGGCAG
GTGCTGGATATGCAGGCAGCGATAGCGCAACTGGAGAATGCGCTGTCGATAGCGAAAAATCTGTCACAGGCGGCGGAAGT
GGCAAAGGCGCACAGCGCTGATCTGTCGGAGCATGAGTCTCTGAATAAGGCATTATCAGAACTGGCAGAGGCGGGAATCG
TGTTGTCTGCCCCGAAGGGCGTGGGGGTAGTGAGTCCTAGAGCGGTGCGTTTGGGTTCCGGGGAAAGCAGTGTGGGACTG
ATGGCGGGAACAAATGTGGACGTGTCGGCGATGAAGAAGATAACACTGACGGCGGGAGATTCAGTGGGAGTGTTTGCCCG
TAAGGACGGGATGAAGCTGTATGCGAATCAGGGAAAAGTGGAAGTGGAAGCACAGAATGGAGGGATGCGCCTGACATCGC
GTAAAGATATGGAGATAAACAGTACGGAAAACCGGGTGGAGATAACGGCAAAAGAAGAGCTGGTGTTGTCGTGCGGCGGT
GCGTATATCCGGCTAAAGGGTGGGAAGATAGAGCTGGGGAGTCCTGGGAATATCCTGCTGAAGTCAGTGAATGTGCAGAA
GATGAGTAAAGCCAGACTGTCACCAGCAATGCCTGTTTTACCGGGGGAAGGCAATTACGATTTGTCGCTGGAGCTTCGGG
ACTGGGAAGGTGCGCCCATCAGCGGTGCGAAGTACAAAATCACCTTTGAATCAGGTTCCGTTCTATCGGGAATACTTGAT
GAGCAGGGATATACACTGCATACCAATGTTCCTCCTGAGTCCGCATCTGTGGAATACGATCTTCCTGACCCGTTACCGGA
TGCCCCCTGGGATCCTTATGCGTCGTTGCTTGCCGCTGTGGATGCTGAATTTTCAACGAACGGACAAGGGGCAAAGTAA