Detailed information of component protein
Summary
| Component ID | T6CP015227 |
| Component protein type | TssL |
| T6SS ID (Type) | T6SS01200  (Type i2) |
| Strain | Escherichia coli KTE165 |
| Replicon | chromosome |
| Sequence | Protein sequence (222 a.a.); Nucleotide sequence (666 bp) |
| Reference | - |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | A31K_RS20855 |
| Coordinate (Strand) | 184372..185037 (+) |
| NCBI ID | 486400239 |
| RefSeq | NZ_KB733141 |
| Uniprot ID | UPI0002A1FA92 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DotU | PF09850.11 | 4.7e-45 | 9..207 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-185 | MKKETDINIDALLRDTFLTVVELRQGTTVRHGMELYRHCQRQVELVRERLKDAGFSRENVEHITYAQCALLDETVLSRSGMDDGQAIWMKNPLQSHFFNTLQAGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPAAPERDQLISTLNGLVAPFGVLPETAVLNVPLSTRQHPLLHS |
| Transmembrane helix | 186-208 | PFFWLVTLTLVLVGVWWGLHYWL |
| Outside | 209-221 | NVLVDELLPQSLR |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9781 | - | - |
Protein sequence: 222 a.a.
>T6CP015227 NZ_KB733141:184372-185037 [Escherichia coli KTE165] [TssL]
MKKETDINIDALLRDTFLTVVELRQGTTVRHGMELYRHCQRQVELVRERLKDAGFSRENVEHITYAQCALLDETVLSRSG
MDDGQAIWMKNPLQSHFFNTLQAGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPAAPERDQLISTLNGLVA
PFGVLPETAVLNVPLSTRQHPLLHSPFFWLVTLTLVLVGVWWGLHYWLNVLVDELLPQSLR*
MKKETDINIDALLRDTFLTVVELRQGTTVRHGMELYRHCQRQVELVRERLKDAGFSRENVEHITYAQCALLDETVLSRSG
MDDGQAIWMKNPLQSHFFNTLQAGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPAAPERDQLISTLNGLVA
PFGVLPETAVLNVPLSTRQHPLLHSPFFWLVTLTLVLVGVWWGLHYWLNVLVDELLPQSLR*
Nucleotide sequence: 666 bp
>T6CP015227 NZ_KB733141:184372-185037 [Escherichia coli KTE165] [TssL]
ATGAAAAAAGAGACGGATATCAATATCGACGCGCTGCTGCGCGATACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAAC
CACCGTGCGTCACGGTATGGAGCTGTATCGCCACTGTCAGCGGCAGGTGGAACTGGTCCGCGAACGTCTGAAAGATGCCG
GATTCAGTCGGGAGAACGTGGAGCACATAACTTACGCGCAGTGCGCCCTGCTGGACGAAACGGTGCTCAGCCGTAGTGGC
ATGGACGACGGTCAGGCCATCTGGATGAAAAACCCACTCCAGTCGCATTTCTTCAACACCCTGCAGGCAGGCGAGCTGCT
GTACGAGCGGATGAAGCAGGTGCTACAGGAGCCCGCCCCCGCGCAGGCCGTGCTGACCTGCTTTCACCGTGTCCTGCTGC
TGGGATTTCGCGGGCGTTATCAGGATCCGGCAGCCCCGGAGCGCGACCAGCTTATTTCGACGCTCAACGGGCTGGTCGCG
CCATTTGGTGTGCTGCCGGAAACGGCGGTACTGAACGTGCCGTTATCCACCCGTCAGCACCCTTTGCTGCATTCGCCGTT
TTTCTGGCTGGTGACGCTCACACTGGTACTGGTCGGTGTCTGGTGGGGGCTACATTACTGGCTGAACGTGCTGGTGGATG
AACTGCTGCCGCAAAGTCTGCGTTAA
ATGAAAAAAGAGACGGATATCAATATCGACGCGCTGCTGCGCGATACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAAC
CACCGTGCGTCACGGTATGGAGCTGTATCGCCACTGTCAGCGGCAGGTGGAACTGGTCCGCGAACGTCTGAAAGATGCCG
GATTCAGTCGGGAGAACGTGGAGCACATAACTTACGCGCAGTGCGCCCTGCTGGACGAAACGGTGCTCAGCCGTAGTGGC
ATGGACGACGGTCAGGCCATCTGGATGAAAAACCCACTCCAGTCGCATTTCTTCAACACCCTGCAGGCAGGCGAGCTGCT
GTACGAGCGGATGAAGCAGGTGCTACAGGAGCCCGCCCCCGCGCAGGCCGTGCTGACCTGCTTTCACCGTGTCCTGCTGC
TGGGATTTCGCGGGCGTTATCAGGATCCGGCAGCCCCGGAGCGCGACCAGCTTATTTCGACGCTCAACGGGCTGGTCGCG
CCATTTGGTGTGCTGCCGGAAACGGCGGTACTGAACGTGCCGTTATCCACCCGTCAGCACCCTTTGCTGCATTCGCCGTT
TTTCTGGCTGGTGACGCTCACACTGGTACTGGTCGGTGTCTGGTGGGGGCTACATTACTGGCTGAACGTGCTGGTGGATG
AACTGCTGCCGCAAAGTCTGCGTTAA