Detailed information of component protein
Summary
| Component ID | T6CP015224 |
| Component protein type | TssB |
| T6SS ID (Type) | T6SS01200  (Type i2) |
| Strain | Escherichia coli KTE165 |
| Replicon | chromosome |
| Sequence | Protein sequence (167 a.a.); Nucleotide sequence (501 bp) |
| Reference | - |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | A31K_RS20870 |
| Coordinate (Strand) | 180921..181421 (+) |
| NCBI ID | WP_001296334.1 |
| RefSeq | NZ_KB733141 |
| Uniprot ID | X5FEF0_ECOLX, A0A5R8T9P2_ECOLX |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS_VipA | PF05591.14 | 5.3e-51 | 7..157 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-166 | MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNGKEQRPLSERDKIDINKNNFNSVMAEFSPAVNLTVEDTLNGNGNEQNIALEFKSLKDFEPEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKIAPQENV |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9943 | - | - |
Protein sequence: 167 a.a.
>T6CP015224 NZ_KB733141:180921-181421 [Escherichia coli KTE165] [TssB]
MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNGKEQRPLSERDKIDINKNNFNSVMAEFSPAVNLTVED
TLNGNGNEQNIALEFKSLKDFEPEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
APQENV*
MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNGKEQRPLSERDKIDINKNNFNSVMAEFSPAVNLTVED
TLNGNGNEQNIALEFKSLKDFEPEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
APQENV*
Nucleotide sequence: 501 bp
>T6CP015224 NZ_KB733141:180921-181421 [Escherichia coli KTE165] [TssB]
ATGGCCGACAGTTTCCAGAATGAAGTTCCCGCCGCTCGTGTAAATATCAAGCTTGATCTGCATACAGGCAATGCTAAAAA
GAAAGTTGAACTCCCCCTCAAGCTTCTTGCCGTAGGCGATTACAGTAACGGAAAAGAGCAGCGTCCGCTGTCCGAACGGG
ACAAGATTGATATCAATAAAAACAACTTCAACAGCGTCATGGCTGAGTTTTCGCCTGCGGTTAATTTAACAGTAGAAGAT
ACGCTAAACGGAAACGGTAATGAACAAAATATCGCGCTTGAATTTAAAAGCCTGAAGGATTTTGAACCCGAACAGGTAGC
AAAAAATATTCCGCAACTTCGTGTATTACTCGCTATGCGCAATTTACTACGTGACCTGAAATCCAATCTTCTGGATAACG
CAACGTTTCGCCGTGAACTGGAAAATATCCTGAAAGACCCGACGCTCAGTTCAGAACTGCGTGATGAACTGGCAAAAATT
GCGCCGCAGGAGAACGTTTAA
ATGGCCGACAGTTTCCAGAATGAAGTTCCCGCCGCTCGTGTAAATATCAAGCTTGATCTGCATACAGGCAATGCTAAAAA
GAAAGTTGAACTCCCCCTCAAGCTTCTTGCCGTAGGCGATTACAGTAACGGAAAAGAGCAGCGTCCGCTGTCCGAACGGG
ACAAGATTGATATCAATAAAAACAACTTCAACAGCGTCATGGCTGAGTTTTCGCCTGCGGTTAATTTAACAGTAGAAGAT
ACGCTAAACGGAAACGGTAATGAACAAAATATCGCGCTTGAATTTAAAAGCCTGAAGGATTTTGAACCCGAACAGGTAGC
AAAAAATATTCCGCAACTTCGTGTATTACTCGCTATGCGCAATTTACTACGTGACCTGAAATCCAATCTTCTGGATAACG
CAACGTTTCGCCGTGAACTGGAAAATATCCTGAAAGACCCGACGCTCAGTTCAGAACTGCGTGATGAACTGGCAAAAATT
GCGCCGCAGGAGAACGTTTAA