Detailed information of component protein
Summary
| Component ID | T6CP015192 |
| Component protein type | TssL |
| T6SS ID (Type) | T6SS01197  (Type i2) |
| Strain | Burkholderia cenocepacia H111 |
| Replicon | chromosome |
| Sequence | Protein sequence (244 a.a.); Nucleotide sequence (732 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | I35_RS17345 |
| Coordinate (Strand) | 178595..179326 (-) |
| NCBI ID | WP_043204570.1 |
| RefSeq | NZ_HG938371 |
| Uniprot ID | UPI0005BCD14A |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DotU | PF09850.11 | 4.9e-43 | 28..225 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-203 | MNTLTSNRSGAALMPNATGASLRPGEGMRDLLRDTALLVTTLTSGGHTQDAVALRARCKQLVEQFASGLQQRGFPDDVRNEALVAQCGLLDEVALRHLPGESRSAWEREPLQVEQFNLHEAGERVFERLDARMREPAPPVALLECYSAILGMGFVGRYARDGEAKRTVLITSLNARLERLRPSLDRPFIAERVERRVSDWFYR |
| Transmembrane helix | 204-226 | LSPWAIAGLACVAAAIVWGVWSA |
| Outside | 227-243 | TLDGQLAHLVAAKAVRP |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.8913 | - | - |
Protein sequence: 244 a.a.
>T6CP015192 NZ_HG938371:c179326-178595 [Burkholderia cenocepacia H111] [TssL]
MNTLTSNRSGAALMPNATGASLRPGEGMRDLLRDTALLVTTLTSGGHTQDAVALRARCKQLVEQFASGLQQRGFPDDVRN
EALVAQCGLLDEVALRHLPGESRSAWEREPLQVEQFNLHEAGERVFERLDARMREPAPPVALLECYSAILGMGFVGRYAR
DGEAKRTVLITSLNARLERLRPSLDRPFIAERVERRVSDWFYRLSPWAIAGLACVAAAIVWGVWSATLDGQLAHLVAAKA
VRP*
MNTLTSNRSGAALMPNATGASLRPGEGMRDLLRDTALLVTTLTSGGHTQDAVALRARCKQLVEQFASGLQQRGFPDDVRN
EALVAQCGLLDEVALRHLPGESRSAWEREPLQVEQFNLHEAGERVFERLDARMREPAPPVALLECYSAILGMGFVGRYAR
DGEAKRTVLITSLNARLERLRPSLDRPFIAERVERRVSDWFYRLSPWAIAGLACVAAAIVWGVWSATLDGQLAHLVAAKA
VRP*
Nucleotide sequence: 732 bp
>T6CP015192 NZ_HG938371:c179326-178595 [Burkholderia cenocepacia H111] [TssL]
ATGAATACCCTGACTTCCAATCGAAGCGGCGCCGCCCTGATGCCGAACGCGACAGGCGCCAGCCTCCGGCCCGGCGAAGG
GATGCGCGATCTGCTGCGCGACACCGCATTGCTCGTGACGACGCTCACCTCGGGCGGACACACGCAGGATGCGGTTGCGT
TGCGGGCCCGGTGCAAGCAACTCGTCGAGCAATTCGCAAGCGGTCTGCAGCAACGGGGCTTTCCGGACGACGTGCGCAAC
GAGGCACTGGTCGCACAGTGCGGGCTGCTCGACGAAGTCGCGCTGCGACACCTGCCCGGCGAATCCCGGTCCGCCTGGGA
GCGCGAGCCGCTGCAGGTGGAACAGTTCAACCTGCACGAAGCCGGTGAACGCGTATTCGAGCGACTCGACGCGCGCATGC
GGGAGCCAGCACCACCCGTCGCGCTGCTCGAGTGCTATTCGGCCATCCTCGGAATGGGATTCGTCGGCCGGTATGCCCGC
GACGGTGAAGCCAAGCGGACTGTGCTGATCACGTCGTTGAATGCCAGGCTGGAAAGGCTGCGTCCTTCCCTGGATCGGCC
ATTCATTGCGGAACGTGTCGAACGACGTGTGTCCGACTGGTTCTACCGACTGTCTCCCTGGGCGATCGCCGGACTCGCGT
GCGTGGCCGCCGCGATCGTGTGGGGCGTCTGGTCGGCCACGCTGGACGGGCAGCTCGCACATCTCGTGGCGGCAAAGGCC
GTTCGCCCGTGA
ATGAATACCCTGACTTCCAATCGAAGCGGCGCCGCCCTGATGCCGAACGCGACAGGCGCCAGCCTCCGGCCCGGCGAAGG
GATGCGCGATCTGCTGCGCGACACCGCATTGCTCGTGACGACGCTCACCTCGGGCGGACACACGCAGGATGCGGTTGCGT
TGCGGGCCCGGTGCAAGCAACTCGTCGAGCAATTCGCAAGCGGTCTGCAGCAACGGGGCTTTCCGGACGACGTGCGCAAC
GAGGCACTGGTCGCACAGTGCGGGCTGCTCGACGAAGTCGCGCTGCGACACCTGCCCGGCGAATCCCGGTCCGCCTGGGA
GCGCGAGCCGCTGCAGGTGGAACAGTTCAACCTGCACGAAGCCGGTGAACGCGTATTCGAGCGACTCGACGCGCGCATGC
GGGAGCCAGCACCACCCGTCGCGCTGCTCGAGTGCTATTCGGCCATCCTCGGAATGGGATTCGTCGGCCGGTATGCCCGC
GACGGTGAAGCCAAGCGGACTGTGCTGATCACGTCGTTGAATGCCAGGCTGGAAAGGCTGCGTCCTTCCCTGGATCGGCC
ATTCATTGCGGAACGTGTCGAACGACGTGTGTCCGACTGGTTCTACCGACTGTCTCCCTGGGCGATCGCCGGACTCGCGT
GCGTGGCCGCCGCGATCGTGTGGGGCGTCTGGTCGGCCACGCTGGACGGGCAGCTCGCACATCTCGTGGCGGCAAAGGCC
GTTCGCCCGTGA