Detailed information of component protein


Summary

Component ID T6CP015179
Component protein type TssJ
T6SS ID (Type) T6SS01196 experimental (Type i4b)
Strain Burkholderia cenocepacia H111
Replicon chromosome
Sequence Protein sequence (200 a.a.); Nucleotide sequence (600 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) I35_RS01710
Coordinate (Strand) 350221..350820 (-)
NCBI ID WP_006485335.1
RefSeq NZ_HG938370
Uniprot ID UPI00017B90FD
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS-SciN PF12790.9 8.1e-30 50..168
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-4 MIRY
Transmembrane helix 5-27 ALPLLACALLAGCAAAPLLGSAA
Outside 28-199 SAVMSAAGIGKPEVPDAQKPPRNIGLTLAAAPNLNAATDNKPLALVVRLYALKDPTSFQQAPFDAFTDPAKEKTALGADLLNVREITLIPGQRYTATEKVSREAQAFGIVALFRDPALQRWKLTFDPVKSEKSGIIIGLHNCAMTVTDGTVIAPQQGAPSQPLNMLSSVSCG


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.8408 pos: 16-17. LAG-CA. MIRYALPLLACALLAG


  Protein sequence: 200 a.a.    

>T6CP015179 NZ_HG938370:c350820-350221 [Burkholderia cenocepacia H111] [TssJ]
MIRYALPLLACALLAGCAAAPLLGSAASAVMSAAGIGKPEVPDAQKPPRNIGLTLAAAPNLNAATDNKPLALVVRLYALK
DPTSFQQAPFDAFTDPAKEKTALGADLLNVREITLIPGQRYTATEKVSREAQAFGIVALFRDPALQRWKLTFDPVKSEKS
GIIIGLHNCAMTVTDGTVIAPQQGAPSQPLNMLSSVSCG*

  Nucleotide sequence: 600 bp    

>T6CP015179 NZ_HG938370:c350820-350221 [Burkholderia cenocepacia H111] [TssJ]
ATGATTCGCTACGCGCTGCCGCTGCTTGCGTGCGCCCTTCTGGCCGGATGCGCGGCCGCTCCGCTGCTCGGCTCGGCCGC
GAGCGCCGTGATGTCCGCGGCCGGCATCGGCAAGCCCGAGGTACCCGACGCGCAGAAGCCGCCGCGCAACATCGGCCTCA
CGCTGGCCGCCGCACCGAACCTGAATGCCGCGACCGACAACAAGCCGCTCGCGCTCGTCGTGCGCCTCTATGCGCTGAAG
GACCCGACCTCGTTCCAGCAAGCGCCGTTCGACGCATTTACCGACCCGGCCAAGGAAAAAACCGCACTCGGCGCCGATCT
GCTGAACGTGCGTGAAATCACGCTGATTCCGGGCCAGCGCTATACGGCAACCGAGAAGGTATCGCGCGAAGCGCAGGCAT
TCGGCATTGTCGCGCTGTTCCGCGATCCCGCACTGCAACGCTGGAAACTGACGTTCGATCCGGTGAAGTCGGAGAAATCC
GGCATAATCATCGGCCTGCATAATTGCGCGATGACGGTCACCGACGGCACGGTCATCGCACCGCAACAGGGTGCGCCTTC
GCAACCGCTGAATATGCTGTCTTCGGTCTCCTGCGGTTAA