Detailed information of component protein


Summary

Component ID T6CP014914
Component protein type TssM
T6SS ID (Type) T6SS01172 experimental (Type i4b)
Strain Acinetobacter baumannii ATCC 17978
Replicon chromosome
Sequence Protein sequence (1275 a.a.); Nucleotide sequence (3825 bp)
Reference
[1] Ruiz FM, Santillana E, Spínola-Amilibia M, et al. Crystal Structure of Hcp from Acinetobacter baumannii: A Component of the Type VI Secretion System. PLoS One. 2015 Jun 16;10(6):e0129691. doi: 10.1371/journal.pone.0129691. eCollection 2015. PMID: 26079269
[2] Weber BS, Miyata ST, Iwashkiw JA, et al. Genomic and functional analysis of the type VI secretion system in Acinetobacter. PLoS One. 2013;8(1):e55142. doi: 10.1371/journal.pone.0055142. Epub 2013 Jan 24. PMID: 23365692
[3] Carruthers MD, Nicholson PA, Tracy EN, et al. Acinetobacter baumannii utilizes a type VI secretion system for bacterial competition. PLoS One. 2013;8(3):e59388. doi: 10.1371/journal.pone.0059388. Epub 2013 Mar 19. PMID: 23527179
[4] Eijkelkamp BA, Stroeher UH, Hassan KA, et al. H-NS plays a role in expression of Acinetobacter baumannii virulence features. Infect Immun. 2013 Jul;81(7):2574-83. doi: 10.1128/IAI.00065-13. Epub 2013 May 6. PMID: 23649094
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) G6543_RS11095
Coordinate (Strand) 2315597..2319421 (-)
NCBI ID WP_000556914.1
RefSeq NZ_CP049363
Uniprot ID A0A7U4F707_ACIBA, A0A0E1PTD4_ACIBA, A0A219CCU5_ACIBA, A0A654KYR3_ACIBM, A0A385EU63_ACIBA
KEGG ID abw:BL01_13240, abz:ABZJ_01461
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related PF06761.14 1.7e-82 496..837
IcmF-related_N PF14331.8 7.8e-79 188..444
IcmF_C PF06744.14 3.8e-18 1107..1197
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-4 MHTI
Transmembrane helix 5-27 LGYLWQYITNPKAIIALSLFVAL
Outside 28-36 MSSYTSIPR
Transmembrane helix 37-59 HIFWALASAYALGLIAYGIYWLI
Inside 60-442 QRKKHAVQGEELAQAIEKDTQAEYGKNKDKEELQLISQQMKESIQLIRKSKLGDKKGNAALYELPWYMVIGNPAAGKSSAIYNSGLKFPFEETHQKMVSAGLSGTRNCDWFFSTEGILLDTAGRYSVYSEDHSEWLGFLNILKKNRSKAPVNGLILIVSIAELVSQSPEHSLKLAKNLRARIQDLTERLEVVVPVYLVFSKMDLIAGFTEFFEFYEAQEYNQAWGATLPYEQNSSHNAVDLFEKHYNILYDGLKGVSSTHLSRRHSQNISPSVMTFPLEFKTLKPALKSFIATLFEDNPYQFQPVFRGFYFTSALQEGVIESPMTEQIAQEFQLSQIAQSEVKDQLKTTPNHGYFLKGLFSDVILKDKDLVKQHVNTSKKRQR
Transmembrane helix 443-462 YIAFIGALAGVSIILGVWVW
Outside 463-1274 SYRNNQQLIAEVQADLNKVVQLEKSSGQQLSTQLEALLILQRRLQQLDEFDENRPLKFSFGLYQGNELREKLKAEYLKGVKQIVLIPTQQNIAQYLQRVKNNEATLKANHTNVEIKQTAQTQQYLEPSDTNPQDAYNALKAYLMMSNPQYMDASHLSDQVTRFWRSWLDANKGQMPRADMIQEAEQILSYAMTLANDKQFPILDADSQLVDQTRQVLLSVIRGMPARDRVYNEIKMRAAVRFPALTVNQIVGDANKNIVLGSYALPGVFTQKAWNEYVEKAIEEAADKPTDTKDWVLNSRQSDDLTFSGSPEQIRKQLTALYKQEYIAEWRKFLSGIHYAKATQFAQQVKNIDVLGEPQNSPIRMLIERVAIETNWDNPVVQAELAAPQKGFIAWFKRKVLNHDDKQLANQAVTNAQGPISQEYQMFYQLVRKRDDQQGKSLLDEYMTNLALVRSKFNELKNAGEIGPNAMTLVKQTLNEQTSVFNQTQKIVDEKMAVGFSEIDQQLLQKLVVSPLTQAFESLITPTQDEINKLWVMQAYQPFTANLAKKYPFNSSASLQATSSEIGQILGENGSISRFVKESLDPFVIRRGYTLTSKTWKDLGISLNPQFVMNFQRYVAPTNGMATGELNSQAPAAPATNQSNFQFYPIQNPQLLSYTVDIDGQRMTYENGVQQWVNFIWPNQGSIPGARITAVDLQGQTHTIFDEPGEYGINRLIDSAQRKEQNGGFEMLWRSKTDPSLFVKMNFRLISSNSGSIGSSRGYSGMQLVDKVTADKAARVVSAQQAPAQAAAPAKTENPVSALAQPAAGVKP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9546 - -


  Protein sequence: 1275 a.a.    

>T6CP014914 NZ_CP049363:c2319421-2315597 [Acinetobacter baumannii] [TssM]
MHTILGYLWQYITNPKAIIALSLFVALMSSYTSIPRHIFWALASAYALGLIAYGIYWLIQRKKHAVQGEELAQAIEKDTQ
AEYGKNKDKEELQLISQQMKESIQLIRKSKLGDKKGNAALYELPWYMVIGNPAAGKSSAIYNSGLKFPFEETHQKMVSAG
LSGTRNCDWFFSTEGILLDTAGRYSVYSEDHSEWLGFLNILKKNRSKAPVNGLILIVSIAELVSQSPEHSLKLAKNLRAR
IQDLTERLEVVVPVYLVFSKMDLIAGFTEFFEFYEAQEYNQAWGATLPYEQNSSHNAVDLFEKHYNILYDGLKGVSSTHL
SRRHSQNISPSVMTFPLEFKTLKPALKSFIATLFEDNPYQFQPVFRGFYFTSALQEGVIESPMTEQIAQEFQLSQIAQSE
VKDQLKTTPNHGYFLKGLFSDVILKDKDLVKQHVNTSKKRQRYIAFIGALAGVSIILGVWVWSYRNNQQLIAEVQADLNK
VVQLEKSSGQQLSTQLEALLILQRRLQQLDEFDENRPLKFSFGLYQGNELREKLKAEYLKGVKQIVLIPTQQNIAQYLQR
VKNNEATLKANHTNVEIKQTAQTQQYLEPSDTNPQDAYNALKAYLMMSNPQYMDASHLSDQVTRFWRSWLDANKGQMPRA
DMIQEAEQILSYAMTLANDKQFPILDADSQLVDQTRQVLLSVIRGMPARDRVYNEIKMRAAVRFPALTVNQIVGDANKNI
VLGSYALPGVFTQKAWNEYVEKAIEEAADKPTDTKDWVLNSRQSDDLTFSGSPEQIRKQLTALYKQEYIAEWRKFLSGIH
YAKATQFAQQVKNIDVLGEPQNSPIRMLIERVAIETNWDNPVVQAELAAPQKGFIAWFKRKVLNHDDKQLANQAVTNAQG
PISQEYQMFYQLVRKRDDQQGKSLLDEYMTNLALVRSKFNELKNAGEIGPNAMTLVKQTLNEQTSVFNQTQKIVDEKMAV
GFSEIDQQLLQKLVVSPLTQAFESLITPTQDEINKLWVMQAYQPFTANLAKKYPFNSSASLQATSSEIGQILGENGSISR
FVKESLDPFVIRRGYTLTSKTWKDLGISLNPQFVMNFQRYVAPTNGMATGELNSQAPAAPATNQSNFQFYPIQNPQLLSY
TVDIDGQRMTYENGVQQWVNFIWPNQGSIPGARITAVDLQGQTHTIFDEPGEYGINRLIDSAQRKEQNGGFEMLWRSKTD
PSLFVKMNFRLISSNSGSIGSSRGYSGMQLVDKVTADKAARVVSAQQAPAQAAAPAKTENPVSALAQPAAGVKP*

  Nucleotide sequence: 3825 bp    

>T6CP014914 NZ_CP049363:c2319421-2315597 [Acinetobacter baumannii] [TssM]
ATGCATACAATTTTAGGCTACTTGTGGCAGTACATCACGAATCCTAAAGCAATTATCGCCTTGTCATTGTTCGTGGCATT
GATGTCTTCCTATACTTCAATTCCTCGCCATATATTTTGGGCTTTAGCATCTGCATATGCTTTAGGGCTCATCGCTTATG
GAATTTATTGGCTAATTCAGCGTAAAAAGCATGCTGTTCAAGGTGAAGAGCTTGCACAAGCGATTGAAAAGGATACCCAA
GCAGAGTACGGCAAAAATAAAGATAAAGAAGAGCTGCAACTTATTAGCCAACAAATGAAAGAATCTATTCAATTGATTCG
TAAGTCAAAGTTAGGCGATAAAAAGGGGAACGCAGCACTATATGAGTTGCCATGGTATATGGTCATTGGTAACCCAGCTG
CTGGTAAAAGTTCTGCGATCTATAATTCTGGTTTAAAGTTTCCTTTTGAAGAAACCCATCAAAAAATGGTTTCGGCTGGT
TTAAGCGGTACGCGTAATTGTGACTGGTTTTTTTCGACTGAGGGTATTTTACTCGACACCGCTGGACGTTATTCGGTCTA
TAGCGAAGACCACTCTGAATGGTTAGGCTTTTTAAATATCTTAAAGAAGAACCGTTCTAAGGCTCCAGTAAATGGTTTAA
TTCTTATTGTTAGTATTGCTGAGCTTGTTAGTCAGAGTCCTGAACACTCATTAAAGCTTGCGAAAAATTTACGCGCTCGT
ATTCAGGATTTAACCGAACGTTTAGAAGTGGTTGTACCCGTATATCTAGTCTTTTCAAAAATGGATTTGATCGCGGGTTT
TACCGAATTTTTCGAATTCTATGAAGCACAAGAATATAATCAGGCGTGGGGCGCAACACTACCATATGAGCAAAACTCTT
CTCATAATGCTGTAGATCTTTTTGAGAAACACTACAATATTCTTTATGACGGTTTAAAAGGGGTCAGTTCAACTCATCTT
AGCCGTCGACATTCTCAAAATATCTCACCTAGTGTGATGACTTTCCCATTAGAATTTAAGACATTAAAACCTGCATTAAA
AAGTTTTATTGCGACATTGTTTGAAGATAACCCTTACCAGTTCCAACCAGTTTTCCGTGGGTTTTATTTCACAAGTGCTT
TGCAAGAAGGCGTAATAGAAAGTCCAATGACTGAGCAAATTGCACAGGAATTCCAGCTTTCCCAAATTGCTCAAAGTGAA
GTAAAAGATCAGCTAAAAACAACGCCTAACCATGGTTATTTCTTAAAAGGCTTGTTTTCAGATGTCATTTTAAAAGATAA
AGATCTGGTTAAACAGCATGTCAATACAAGCAAAAAGCGTCAGCGTTATATTGCATTTATTGGTGCTTTAGCAGGCGTAT
CCATTATTCTCGGTGTGTGGGTTTGGTCATATCGTAATAATCAACAACTTATTGCCGAAGTTCAAGCTGATTTGAATAAA
GTTGTTCAATTGGAAAAGAGTTCAGGTCAACAATTGTCGACTCAATTAGAGGCCTTACTTATCTTGCAAAGACGTCTACA
ACAGTTAGACGAATTTGATGAAAATAGACCACTAAAATTTAGTTTTGGTTTATATCAAGGTAATGAGCTACGTGAAAAAT
TAAAAGCTGAATATTTAAAAGGTGTTAAGCAAATTGTTTTGATACCTACACAGCAAAATATTGCTCAGTATTTGCAACGT
GTAAAAAATAACGAAGCAACTTTAAAAGCAAATCATACCAATGTTGAGATCAAGCAAACTGCTCAAACTCAGCAATATTT
AGAGCCTTCGGATACCAACCCTCAAGATGCTTATAATGCTTTAAAAGCATATTTAATGATGAGCAATCCACAGTATATGG
ATGCAAGTCACTTAAGTGATCAGGTAACGCGTTTCTGGCGTTCTTGGCTTGATGCCAATAAAGGGCAGATGCCACGTGCA
GATATGATCCAAGAAGCTGAGCAGATCTTATCTTATGCAATGACATTGGCAAATGATAAACAATTCCCGATCTTGGATGC
TGACTCTCAACTCGTGGATCAGACACGTCAAGTATTGCTCTCTGTTATTAGAGGTATGCCAGCACGTGACCGTGTTTATA
ATGAAATTAAAATGCGTGCTGCTGTGCGCTTTCCAGCTTTAACTGTAAACCAGATTGTAGGTGACGCAAATAAAAATATT
GTATTGGGTAGTTATGCATTACCAGGGGTATTTACTCAAAAAGCTTGGAACGAGTATGTAGAAAAAGCAATTGAGGAAGC
AGCAGACAAACCTACGGATACTAAAGACTGGGTGCTTAATAGCCGTCAATCTGATGATTTAACTTTCTCAGGTAGTCCAG
AGCAAATCAGAAAGCAGCTTACTGCATTGTATAAACAAGAATATATTGCAGAGTGGAGAAAATTCTTAAGTGGCATTCAC
TATGCAAAAGCAACTCAGTTTGCTCAGCAAGTTAAAAATATTGATGTATTGGGCGAACCACAAAATTCACCAATTCGCAT
GCTTATTGAACGCGTAGCCATTGAAACAAACTGGGATAATCCGGTTGTTCAAGCAGAGTTAGCAGCGCCTCAAAAAGGAT
TTATTGCATGGTTTAAACGTAAGGTTTTAAACCATGATGATAAACAATTAGCAAATCAGGCTGTAACGAATGCTCAAGGG
CCTATTTCTCAAGAATATCAAATGTTCTATCAATTGGTTCGTAAACGTGATGATCAGCAAGGTAAATCTTTGCTTGATGA
ATATATGACGAATCTTGCCTTAGTACGTAGTAAGTTTAATGAACTTAAGAATGCGGGTGAGATCGGACCGAATGCGATGA
CATTGGTTAAGCAAACTTTAAATGAGCAGACATCGGTATTTAACCAGACTCAAAAAATTGTAGATGAAAAGATGGCGGTT
GGTTTCAGTGAAATTGATCAGCAACTTCTACAAAAATTAGTGGTGAGTCCACTTACCCAAGCCTTTGAAAGTTTAATTAC
ACCTACTCAAGATGAAATTAATAAGCTATGGGTAATGCAAGCCTATCAACCATTTACTGCAAATCTTGCTAAGAAATATC
CATTTAACTCATCAGCTTCTTTACAAGCAACAAGTAGTGAAATTGGACAAATTCTTGGTGAGAACGGCAGTATTTCCCGT
TTTGTTAAAGAGAGTTTAGACCCGTTTGTTATCCGCCGCGGTTATACCTTAACCTCCAAAACATGGAAAGATTTGGGGAT
TAGTTTAAACCCACAATTTGTTATGAACTTCCAACGCTATGTTGCACCTACAAATGGAATGGCAACAGGTGAGTTAAATA
GCCAAGCACCAGCTGCTCCGGCAACTAATCAGTCTAACTTCCAGTTCTACCCAATTCAAAACCCGCAGTTACTTTCATAT
ACCGTTGATATTGATGGTCAGCGTATGACTTATGAAAATGGGGTGCAGCAATGGGTTAACTTTATTTGGCCAAATCAAGG
CAGTATTCCGGGTGCTCGTATTACGGCTGTAGATTTACAAGGTCAAACACATACCATTTTTGATGAACCAGGCGAATACG
GGATTAACCGTTTAATCGATAGTGCACAGCGTAAAGAGCAAAACGGTGGTTTTGAAATGCTTTGGCGCAGTAAGACAGAT
CCATCACTCTTTGTGAAAATGAACTTCCGTCTTATTAGTAGTAATTCAGGAAGTATCGGTTCAAGCCGTGGCTACTCTGG
TATGCAACTGGTTGACAAAGTAACTGCGGATAAGGCTGCTCGCGTTGTATCCGCACAGCAAGCACCAGCTCAGGCAGCTG
CTCCTGCCAAAACTGAAAATCCAGTTTCTGCACTAGCACAACCTGCTGCGGGAGTTAAGCCATGA