Detailed information of component protein


Summary

Component ID T6CP014895
Component protein type TssA
T6SS ID (Type) T6SS01170 experimental (Type i1)
Strain Pectobacterium brasiliense 1692
Replicon chromosome
Sequence Protein sequence (479 a.a.); Nucleotide sequence (1437 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) GT391_RS12345
Coordinate (Strand) 2768182..2769618 (+)
NCBI ID WP_010285391.1
RefSeq NZ_CP047495
Uniprot ID A0A6I6WW95_9GAMM
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
VasL PF12486.10 1.5e-54 326..472
ImpA_N PF06812.14 1.9e-22 5..112
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-253 MQDAQQALKVGRDPRMLPEFDALRAEINKLSHASRPDVDWMLVHDMATTIFEKQGVDLQTAIYFTLARSRLAGLTGFTESCEFLANLIVTQWDNFWPPVHQERARIEMLDWFIARISEVVRQYAISHEHKRLVYRCERALQLMSEKLHNSGLSRIPRVENLLHFVEGYTHLFDETEIVIVSDDQELKKQDMQIPPMVFFHSDMEPGATVQSSGSSGSGQAALPAGSILIGREKGQMKPTVLKIEAHKKQKPAW
Transmembrane helix 254-276 FWFVSGLLTCALPVAAITGWQYW
Outside 277-478 QEQKADALALLQQPAYALPAAPDHNDIRRVRIALGEQKLQGMEGELINRYQAQLELVKNASPFYLYQYGNGLKNVMQQLYPDSLAVKEMERQWQIALERQQGDEPKTRGYEQARARVNDTLQQLLELERQRRTVTISYLKSKLYDMQKDLISDVPFGIRLRELEARKTRSQSLTPAELRAMEDELRAFNVRLYRLQQGNSAS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9971 - -


  Protein sequence: 479 a.a.    

>T6CP014895 NZ_CP047495:2768182-2769618 [Pectobacterium brasiliense] [TssA]
MQDAQQALKVGRDPRMLPEFDALRAEINKLSHASRPDVDWMLVHDMATTIFEKQGVDLQTAIYFTLARSRLAGLTGFTES
CEFLANLIVTQWDNFWPPVHQERARIEMLDWFIARISEVVRQYAISHEHKRLVYRCERALQLMSEKLHNSGLSRIPRVEN
LLHFVEGYTHLFDETEIVIVSDDQELKKQDMQIPPMVFFHSDMEPGATVQSSGSSGSGQAALPAGSILIGREKGQMKPTV
LKIEAHKKQKPAWFWFVSGLLTCALPVAAITGWQYWQEQKADALALLQQPAYALPAAPDHNDIRRVRIALGEQKLQGMEG
ELINRYQAQLELVKNASPFYLYQYGNGLKNVMQQLYPDSLAVKEMERQWQIALERQQGDEPKTRGYEQARARVNDTLQQL
LELERQRRTVTISYLKSKLYDMQKDLISDVPFGIRLRELEARKTRSQSLTPAELRAMEDELRAFNVRLYRLQQGNSAS*

  Nucleotide sequence: 1437 bp    

>T6CP014895 NZ_CP047495:2768182-2769618 [Pectobacterium brasiliense] [TssA]
ATGCAAGATGCACAACAGGCTCTGAAAGTCGGCCGCGATCCACGGATGCTGCCGGAGTTTGACGCACTTCGGGCGGAAAT
TAACAAGTTGAGCCACGCGTCTCGCCCTGATGTGGATTGGATGCTGGTGCACGATATGGCCACCACCATCTTTGAAAAAC
AGGGCGTGGATCTCCAGACCGCGATTTACTTTACGCTGGCGCGTTCGCGGCTGGCTGGGTTGACGGGCTTCACGGAAAGC
TGTGAGTTTCTGGCGAATTTGATCGTGACGCAGTGGGATAACTTCTGGCCGCCGGTACATCAGGAACGGGCGCGTATCGA
GATGCTGGACTGGTTTATTGCTCGCATCAGCGAAGTGGTGCGTCAGTATGCCATCAGCCATGAACATAAGCGGCTGGTTT
ACCGTTGCGAACGTGCACTGCAATTGATGAGTGAAAAGCTGCACAACTCGGGGCTGAGCCGCATTCCTCGCGTCGAGAAT
CTGCTGCACTTCGTCGAAGGCTATACCCATTTGTTTGATGAAACCGAGATTGTCATTGTGTCGGACGATCAGGAACTGAA
AAAGCAGGATATGCAGATTCCACCCATGGTGTTCTTTCATTCGGACATGGAGCCGGGCGCGACGGTGCAAAGCAGTGGTT
CATCAGGCTCGGGTCAGGCTGCTCTGCCTGCGGGCAGTATTCTCATCGGACGGGAGAAAGGGCAAATGAAACCGACGGTA
TTGAAGATTGAGGCGCATAAAAAGCAGAAACCCGCGTGGTTTTGGTTTGTCTCCGGGCTGCTGACCTGCGCGCTGCCTGT
CGCGGCCATTACAGGCTGGCAGTATTGGCAGGAACAGAAAGCCGATGCGCTGGCACTATTGCAACAGCCTGCATACGCCT
TGCCTGCCGCCCCCGATCATAATGACATTCGCCGAGTGCGTATTGCGCTGGGCGAGCAGAAATTGCAGGGCATGGAAGGT
GAGCTAATTAACCGCTATCAGGCACAGCTGGAGCTGGTGAAAAATGCGTCGCCGTTCTATCTGTATCAATACGGAAACGG
CTTGAAAAACGTGATGCAGCAGCTCTATCCCGACTCGCTGGCGGTGAAAGAGATGGAACGCCAGTGGCAGATTGCGCTTG
AACGTCAGCAGGGCGATGAACCAAAAACACGCGGGTACGAACAGGCCCGTGCCAGAGTCAACGACACGTTGCAGCAACTG
CTGGAACTGGAGCGACAGCGCCGGACGGTGACGATTTCCTACCTGAAATCAAAGCTCTACGATATGCAAAAAGACCTGAT
ATCGGACGTTCCCTTTGGGATACGGCTGCGGGAGCTTGAGGCGCGTAAAACCAGGAGCCAGTCGCTGACGCCTGCGGAGC
TACGCGCGATGGAAGACGAACTGCGCGCCTTCAACGTTCGCTTGTACCGTTTGCAGCAGGGTAATTCTGCCAGCTGA