Detailed information of component protein
Summary
Component ID | T6CP014894 |
Component protein type | TssM |
T6SS ID (Type) | T6SS01170 (Type i1) |
Strain | Pectobacterium brasiliense 1692 |
Replicon | chromosome |
Sequence | Protein sequence (1166 a.a.); Nucleotide sequence (3498 bp) |
Reference |
External database links
Locus tag (Gene) | GT391_RS12340 |
Coordinate (Strand) | 2764632..2768129 (+) |
NCBI ID | WP_039320559.1 |
RefSeq | NZ_CP047495 |
Uniprot ID | A0A6I6X5N5_9GAMM |
KEGG ID | - |
PDB ID | - |
Pfam domain hit(s)
Domain | Pfam ID | E-value | Aligned region |
---|---|---|---|
IcmF-related | PF06761.14 | 1.9e-79 | 506..819 |
IcmF-related_N | PF14331.8 | 2.3e-68 | 201..452 |
IcmF_C | PF06744.14 | 2.4e-23 | 1038..1144 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
Prediction | Region | Sequence |
---|---|---|
Inside | 1-19 | MFKTIITFLRKQLPKLKPS |
Transmembrane helix | 20-39 | WLLLGVLLWVVVLVLAWWLG |
Outside | 40-53 | PRLTVGESRPLQGI |
Transmembrane helix | 54-76 | WGRVVFTLVWLWLAFSYSAWLVW |
Inside | 77-449 | RRVQQMRAERHEQQVIEQDPLQVYVDSQQTFLDRWLEAFQTQLGKRALYAMPWYLTIGLAGSGKSSLIHRANPANKMNPKLDAELRDVAAGQQVSSWVGESAVIWDPSGQLLAQPELEGDSLGQRHARLWQHLLQWLSENRRRQPLNGLVLTLDISWLAQAGVADRKAYAQVMRARLQEISVNINTRLPVYIALTRLDMLSGFDKVYRQLNREARQAVLGVTFTPQASNSKGWLEELERFWDEWVTHLNDNLPDMLLTQSDRSVRNSLFSFVRQLAGVKDYVMEVLTETLATGEDRAFLIRGVYVSSVYQQGVPFDAFAQSASRRYQLPEPINPALRGESNTFFVQRLFPDVIFPEASLAGENRLHSLYRRRR |
Transmembrane helix | 450-472 | LSIGVGCMLLASLALIGSWHHFY |
Outside | 473-1165 | RVNEEAGRNVLTKAQAFIGTNELEGQQGYGYQQLPRLNLIRDATLSFGNYHERTPLLADLGLYQGDKIGPHVEGTYLQMLNQRFLPAVMQGLLEDLNQAPANSEQKLTILRVMRMLDDASGRNKALVEQFMAQRWQKAFPGQGNVQEQLMQHLDYALEHTDWHKAREQKDAVAISTFAPFNGPITQAQQELSKLPMYQRVYQSLVMKATQVLPPDLAIRDEVGPTFDPVFSLRNDKAGSVPRLLTYPGFSDYYLKQDKALLELTALDAWVLGQRERAQFSEADRREILRQVNDRYITDYINQWQKVLANIDVQTLDTPEQALDILTDITGNDQPFQRVLTTVSDNTRIRKLADDDNDTAQSINTRTGRPFMTINAALSGRGEQGPLVQEVNQKLTDLYHYLDQIVNATDPGQAALKAVQARQGNKFADPVFALQQYARSLPAPLDRWVGQLAGESASLVTGLAMSSLNQEWLDKVVTPFNEKLADRYPFDPSSNKDVPLSEMEMFFMAGGTLDSFYQTNLKAMMESGMLEEGMASPLQAELVKQLERATRIRQTLFNAQGSLEVHFVLEPLELTANKRRSVLNLDGQLLEYSHGRRQKTPLVWPNSMRDGAESKLTLVPDDRERSPRSLSFSGPWAMFRLINSDQLTQVNENTFDVRFSLENGAMTYRVYTDASHNPFAGGLFSQFTLPDSLY |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction | Probability | Cleavage site | Signal peptide sequence |
---|---|---|---|
Other | 0.9955 | - | - |
Protein sequence: 1166 a.a.
>T6CP014894 NZ_CP047495:2764632-2768129 [Pectobacterium brasiliense] [TssM]
MFKTIITFLRKQLPKLKPSWLLLGVLLWVVVLVLAWWLGPRLTVGESRPLQGIWGRVVFTLVWLWLAFSYSAWLVWRRVQ
QMRAERHEQQVIEQDPLQVYVDSQQTFLDRWLEAFQTQLGKRALYAMPWYLTIGLAGSGKSSLIHRANPANKMNPKLDAE
LRDVAAGQQVSSWVGESAVIWDPSGQLLAQPELEGDSLGQRHARLWQHLLQWLSENRRRQPLNGLVLTLDISWLAQAGVA
DRKAYAQVMRARLQEISVNINTRLPVYIALTRLDMLSGFDKVYRQLNREARQAVLGVTFTPQASNSKGWLEELERFWDEW
VTHLNDNLPDMLLTQSDRSVRNSLFSFVRQLAGVKDYVMEVLTETLATGEDRAFLIRGVYVSSVYQQGVPFDAFAQSASR
RYQLPEPINPALRGESNTFFVQRLFPDVIFPEASLAGENRLHSLYRRRRLSIGVGCMLLASLALIGSWHHFYRVNEEAGR
NVLTKAQAFIGTNELEGQQGYGYQQLPRLNLIRDATLSFGNYHERTPLLADLGLYQGDKIGPHVEGTYLQMLNQRFLPAV
MQGLLEDLNQAPANSEQKLTILRVMRMLDDASGRNKALVEQFMAQRWQKAFPGQGNVQEQLMQHLDYALEHTDWHKAREQ
KDAVAISTFAPFNGPITQAQQELSKLPMYQRVYQSLVMKATQVLPPDLAIRDEVGPTFDPVFSLRNDKAGSVPRLLTYPG
FSDYYLKQDKALLELTALDAWVLGQRERAQFSEADRREILRQVNDRYITDYINQWQKVLANIDVQTLDTPEQALDILTDI
TGNDQPFQRVLTTVSDNTRIRKLADDDNDTAQSINTRTGRPFMTINAALSGRGEQGPLVQEVNQKLTDLYHYLDQIVNAT
DPGQAALKAVQARQGNKFADPVFALQQYARSLPAPLDRWVGQLAGESASLVTGLAMSSLNQEWLDKVVTPFNEKLADRYP
FDPSSNKDVPLSEMEMFFMAGGTLDSFYQTNLKAMMESGMLEEGMASPLQAELVKQLERATRIRQTLFNAQGSLEVHFVL
EPLELTANKRRSVLNLDGQLLEYSHGRRQKTPLVWPNSMRDGAESKLTLVPDDRERSPRSLSFSGPWAMFRLINSDQLTQ
VNENTFDVRFSLENGAMTYRVYTDASHNPFAGGLFSQFTLPDSLY*
MFKTIITFLRKQLPKLKPSWLLLGVLLWVVVLVLAWWLGPRLTVGESRPLQGIWGRVVFTLVWLWLAFSYSAWLVWRRVQ
QMRAERHEQQVIEQDPLQVYVDSQQTFLDRWLEAFQTQLGKRALYAMPWYLTIGLAGSGKSSLIHRANPANKMNPKLDAE
LRDVAAGQQVSSWVGESAVIWDPSGQLLAQPELEGDSLGQRHARLWQHLLQWLSENRRRQPLNGLVLTLDISWLAQAGVA
DRKAYAQVMRARLQEISVNINTRLPVYIALTRLDMLSGFDKVYRQLNREARQAVLGVTFTPQASNSKGWLEELERFWDEW
VTHLNDNLPDMLLTQSDRSVRNSLFSFVRQLAGVKDYVMEVLTETLATGEDRAFLIRGVYVSSVYQQGVPFDAFAQSASR
RYQLPEPINPALRGESNTFFVQRLFPDVIFPEASLAGENRLHSLYRRRRLSIGVGCMLLASLALIGSWHHFYRVNEEAGR
NVLTKAQAFIGTNELEGQQGYGYQQLPRLNLIRDATLSFGNYHERTPLLADLGLYQGDKIGPHVEGTYLQMLNQRFLPAV
MQGLLEDLNQAPANSEQKLTILRVMRMLDDASGRNKALVEQFMAQRWQKAFPGQGNVQEQLMQHLDYALEHTDWHKAREQ
KDAVAISTFAPFNGPITQAQQELSKLPMYQRVYQSLVMKATQVLPPDLAIRDEVGPTFDPVFSLRNDKAGSVPRLLTYPG
FSDYYLKQDKALLELTALDAWVLGQRERAQFSEADRREILRQVNDRYITDYINQWQKVLANIDVQTLDTPEQALDILTDI
TGNDQPFQRVLTTVSDNTRIRKLADDDNDTAQSINTRTGRPFMTINAALSGRGEQGPLVQEVNQKLTDLYHYLDQIVNAT
DPGQAALKAVQARQGNKFADPVFALQQYARSLPAPLDRWVGQLAGESASLVTGLAMSSLNQEWLDKVVTPFNEKLADRYP
FDPSSNKDVPLSEMEMFFMAGGTLDSFYQTNLKAMMESGMLEEGMASPLQAELVKQLERATRIRQTLFNAQGSLEVHFVL
EPLELTANKRRSVLNLDGQLLEYSHGRRQKTPLVWPNSMRDGAESKLTLVPDDRERSPRSLSFSGPWAMFRLINSDQLTQ
VNENTFDVRFSLENGAMTYRVYTDASHNPFAGGLFSQFTLPDSLY*
Nucleotide sequence: 3498 bp
>T6CP014894 NZ_CP047495:2764632-2768129 [Pectobacterium brasiliense] [TssM]
ATGTTTAAAACAATCATAACTTTTCTACGCAAGCAGTTGCCTAAACTGAAACCCTCCTGGTTACTTTTGGGCGTGCTGCT
GTGGGTCGTCGTGTTGGTTCTGGCCTGGTGGCTGGGGCCGCGTTTGACGGTGGGCGAGTCGCGCCCGTTGCAGGGAATCT
GGGGCCGCGTGGTATTCACGCTGGTTTGGCTGTGGCTGGCGTTTTCCTACAGCGCCTGGCTGGTCTGGCGTCGCGTGCAG
CAGATGCGGGCGGAGCGTCATGAGCAGCAGGTGATTGAACAGGATCCCTTGCAGGTGTATGTCGATAGCCAGCAAACATT
TCTCGATCGCTGGCTGGAGGCGTTTCAGACCCAGTTGGGTAAACGCGCGCTGTACGCGATGCCCTGGTATCTGACGATTG
GTCTGGCTGGCAGCGGGAAAAGTAGCCTGATCCATCGTGCTAATCCGGCAAACAAAATGAATCCGAAGCTGGATGCGGAG
CTGCGAGACGTGGCGGCAGGTCAGCAGGTGAGCAGTTGGGTCGGTGAATCCGCGGTGATTTGGGACCCAAGCGGGCAGCT
ACTTGCCCAGCCTGAGCTGGAAGGCGATTCGCTCGGACAGCGTCATGCTCGACTGTGGCAGCACCTGTTGCAGTGGCTCA
GTGAAAACCGCCGCCGCCAGCCGCTCAACGGTCTGGTGCTCACGCTGGATATTTCCTGGCTGGCGCAGGCTGGCGTCGCC
GATCGCAAAGCTTACGCGCAGGTGATGCGCGCCCGCTTGCAGGAAATTTCCGTCAACATCAATACGCGTTTACCGGTGTA
TATCGCGCTGACCCGGCTGGATATGCTGAGCGGCTTCGATAAGGTCTATCGCCAGTTGAACCGCGAGGCGCGTCAGGCCG
TATTAGGGGTGACGTTTACGCCGCAGGCGAGCAACAGCAAAGGCTGGTTAGAAGAGCTGGAGCGCTTCTGGGACGAGTGG
GTCACGCACCTGAATGACAACCTGCCGGATATGCTGCTGACGCAATCCGACAGAAGCGTGCGCAATTCGCTGTTCTCGTT
CGTTCGCCAGTTGGCTGGAGTGAAAGATTACGTGATGGAAGTGCTGACAGAAACGTTAGCGACGGGCGAGGATCGCGCGT
TCCTGATCCGCGGCGTCTACGTCAGCTCGGTCTACCAACAAGGGGTGCCGTTCGATGCCTTTGCGCAATCGGCTTCCCGA
CGCTATCAACTGCCAGAGCCGATTAACCCTGCGCTGCGCGGTGAATCGAATACGTTCTTTGTGCAGCGTCTCTTCCCTGA
CGTCATTTTTCCGGAGGCCAGTCTGGCTGGGGAAAACCGGCTGCATAGCCTCTATCGCCGGCGTCGGCTGAGTATTGGCG
TGGGCTGCATGTTACTTGCCAGTCTGGCGCTGATTGGCAGTTGGCACCATTTCTATCGGGTCAATGAAGAGGCGGGACGT
AACGTCCTGACGAAAGCGCAGGCGTTCATTGGCACCAATGAGCTGGAAGGCCAGCAGGGTTACGGCTATCAGCAGTTGCC
GCGTCTGAATTTGATTCGCGATGCGACCTTATCCTTTGGTAATTACCACGAGCGCACGCCGCTGCTGGCCGATCTTGGCC
TGTATCAGGGTGACAAGATTGGGCCTCATGTTGAAGGCACCTATCTGCAAATGCTGAATCAGCGCTTTCTCCCGGCAGTG
ATGCAAGGGCTGCTGGAAGATCTGAATCAGGCACCGGCCAATAGCGAGCAAAAACTGACGATTCTGCGCGTGATGCGGAT
GCTGGATGATGCGTCAGGGCGCAATAAAGCGCTGGTCGAACAGTTTATGGCGCAGCGCTGGCAGAAGGCATTTCCCGGAC
AGGGCAACGTGCAGGAACAGCTGATGCAGCATCTGGATTACGCCCTTGAGCACACCGACTGGCACAAGGCACGTGAGCAG
AAAGACGCGGTAGCGATCAGCACGTTCGCACCATTTAATGGACCGATTACGCAGGCCCAGCAGGAACTGAGCAAGCTGCC
AATGTATCAGCGCGTTTATCAGAGTCTGGTCATGAAGGCGACACAGGTGCTGCCACCGGATTTAGCGATTCGTGATGAAG
TCGGGCCGACCTTTGATCCGGTGTTTTCCCTGCGTAATGACAAAGCGGGCAGCGTGCCTCGGCTGCTGACGTATCCCGGC
TTTAGCGATTATTACCTCAAGCAGGACAAAGCGCTGTTAGAGCTGACGGCACTGGACGCCTGGGTGCTGGGACAGCGCGA
GCGTGCGCAGTTCAGCGAGGCCGATCGGCGGGAAATTCTGCGTCAGGTGAATGACCGCTACATTACGGATTACATCAACC
AATGGCAGAAAGTGCTGGCGAACATTGATGTGCAGACGCTCGATACGCCGGAGCAGGCACTCGACATTCTCACGGATATT
ACCGGCAACGATCAGCCCTTCCAGCGCGTGCTGACGACGGTCAGTGATAACACCCGCATTCGTAAGCTGGCCGATGATGA
TAACGATACGGCGCAGAGTATTAACACGCGTACTGGTCGTCCGTTTATGACTATCAATGCGGCGCTGAGCGGACGCGGTG
AGCAGGGGCCGCTGGTGCAAGAGGTCAATCAGAAGCTGACGGATCTCTATCACTATCTCGATCAGATCGTTAACGCGACC
GATCCGGGACAGGCGGCGTTGAAAGCGGTGCAGGCACGGCAGGGGAATAAATTTGCCGATCCGGTATTCGCCTTACAGCA
ATACGCCCGTAGCCTTCCTGCGCCGCTGGATCGCTGGGTAGGGCAATTAGCCGGAGAGAGCGCGAGTCTGGTGACCGGGT
TGGCGATGTCGTCGCTGAATCAGGAATGGCTGGATAAGGTCGTGACGCCATTCAATGAGAAGCTGGCGGATCGTTACCCC
TTCGACCCGTCATCGAACAAGGATGTGCCGCTATCGGAAATGGAAATGTTCTTCATGGCCGGCGGGACGTTGGACAGCTT
CTATCAAACCAACCTCAAGGCGATGATGGAGAGCGGCATGCTGGAAGAAGGCATGGCATCCCCTCTGCAGGCAGAGCTGG
TGAAACAGCTTGAACGCGCTACCCGCATCCGCCAGACCCTGTTTAATGCGCAGGGCAGCCTGGAAGTACATTTCGTTCTG
GAACCGCTGGAACTGACGGCGAACAAGCGCCGCAGCGTGCTGAATCTGGACGGACAGCTGCTGGAATACAGTCACGGTCG
TCGCCAGAAAACGCCGTTGGTGTGGCCAAACAGTATGCGCGACGGGGCTGAGAGCAAGCTGACGCTGGTGCCGGACGATC
GTGAGCGTTCACCGCGCAGCCTGAGCTTTAGCGGGCCGTGGGCGATGTTCCGCTTAATCAATAGCGATCAGCTGACTCAG
GTGAATGAGAACACCTTTGACGTGCGCTTCTCGCTGGAAAATGGCGCGATGACGTATCGCGTGTATACCGATGCCAGCCA
TAACCCGTTTGCCGGTGGTCTGTTCAGCCAGTTCACGCTGCCTGACTCGCTTTATTAA
ATGTTTAAAACAATCATAACTTTTCTACGCAAGCAGTTGCCTAAACTGAAACCCTCCTGGTTACTTTTGGGCGTGCTGCT
GTGGGTCGTCGTGTTGGTTCTGGCCTGGTGGCTGGGGCCGCGTTTGACGGTGGGCGAGTCGCGCCCGTTGCAGGGAATCT
GGGGCCGCGTGGTATTCACGCTGGTTTGGCTGTGGCTGGCGTTTTCCTACAGCGCCTGGCTGGTCTGGCGTCGCGTGCAG
CAGATGCGGGCGGAGCGTCATGAGCAGCAGGTGATTGAACAGGATCCCTTGCAGGTGTATGTCGATAGCCAGCAAACATT
TCTCGATCGCTGGCTGGAGGCGTTTCAGACCCAGTTGGGTAAACGCGCGCTGTACGCGATGCCCTGGTATCTGACGATTG
GTCTGGCTGGCAGCGGGAAAAGTAGCCTGATCCATCGTGCTAATCCGGCAAACAAAATGAATCCGAAGCTGGATGCGGAG
CTGCGAGACGTGGCGGCAGGTCAGCAGGTGAGCAGTTGGGTCGGTGAATCCGCGGTGATTTGGGACCCAAGCGGGCAGCT
ACTTGCCCAGCCTGAGCTGGAAGGCGATTCGCTCGGACAGCGTCATGCTCGACTGTGGCAGCACCTGTTGCAGTGGCTCA
GTGAAAACCGCCGCCGCCAGCCGCTCAACGGTCTGGTGCTCACGCTGGATATTTCCTGGCTGGCGCAGGCTGGCGTCGCC
GATCGCAAAGCTTACGCGCAGGTGATGCGCGCCCGCTTGCAGGAAATTTCCGTCAACATCAATACGCGTTTACCGGTGTA
TATCGCGCTGACCCGGCTGGATATGCTGAGCGGCTTCGATAAGGTCTATCGCCAGTTGAACCGCGAGGCGCGTCAGGCCG
TATTAGGGGTGACGTTTACGCCGCAGGCGAGCAACAGCAAAGGCTGGTTAGAAGAGCTGGAGCGCTTCTGGGACGAGTGG
GTCACGCACCTGAATGACAACCTGCCGGATATGCTGCTGACGCAATCCGACAGAAGCGTGCGCAATTCGCTGTTCTCGTT
CGTTCGCCAGTTGGCTGGAGTGAAAGATTACGTGATGGAAGTGCTGACAGAAACGTTAGCGACGGGCGAGGATCGCGCGT
TCCTGATCCGCGGCGTCTACGTCAGCTCGGTCTACCAACAAGGGGTGCCGTTCGATGCCTTTGCGCAATCGGCTTCCCGA
CGCTATCAACTGCCAGAGCCGATTAACCCTGCGCTGCGCGGTGAATCGAATACGTTCTTTGTGCAGCGTCTCTTCCCTGA
CGTCATTTTTCCGGAGGCCAGTCTGGCTGGGGAAAACCGGCTGCATAGCCTCTATCGCCGGCGTCGGCTGAGTATTGGCG
TGGGCTGCATGTTACTTGCCAGTCTGGCGCTGATTGGCAGTTGGCACCATTTCTATCGGGTCAATGAAGAGGCGGGACGT
AACGTCCTGACGAAAGCGCAGGCGTTCATTGGCACCAATGAGCTGGAAGGCCAGCAGGGTTACGGCTATCAGCAGTTGCC
GCGTCTGAATTTGATTCGCGATGCGACCTTATCCTTTGGTAATTACCACGAGCGCACGCCGCTGCTGGCCGATCTTGGCC
TGTATCAGGGTGACAAGATTGGGCCTCATGTTGAAGGCACCTATCTGCAAATGCTGAATCAGCGCTTTCTCCCGGCAGTG
ATGCAAGGGCTGCTGGAAGATCTGAATCAGGCACCGGCCAATAGCGAGCAAAAACTGACGATTCTGCGCGTGATGCGGAT
GCTGGATGATGCGTCAGGGCGCAATAAAGCGCTGGTCGAACAGTTTATGGCGCAGCGCTGGCAGAAGGCATTTCCCGGAC
AGGGCAACGTGCAGGAACAGCTGATGCAGCATCTGGATTACGCCCTTGAGCACACCGACTGGCACAAGGCACGTGAGCAG
AAAGACGCGGTAGCGATCAGCACGTTCGCACCATTTAATGGACCGATTACGCAGGCCCAGCAGGAACTGAGCAAGCTGCC
AATGTATCAGCGCGTTTATCAGAGTCTGGTCATGAAGGCGACACAGGTGCTGCCACCGGATTTAGCGATTCGTGATGAAG
TCGGGCCGACCTTTGATCCGGTGTTTTCCCTGCGTAATGACAAAGCGGGCAGCGTGCCTCGGCTGCTGACGTATCCCGGC
TTTAGCGATTATTACCTCAAGCAGGACAAAGCGCTGTTAGAGCTGACGGCACTGGACGCCTGGGTGCTGGGACAGCGCGA
GCGTGCGCAGTTCAGCGAGGCCGATCGGCGGGAAATTCTGCGTCAGGTGAATGACCGCTACATTACGGATTACATCAACC
AATGGCAGAAAGTGCTGGCGAACATTGATGTGCAGACGCTCGATACGCCGGAGCAGGCACTCGACATTCTCACGGATATT
ACCGGCAACGATCAGCCCTTCCAGCGCGTGCTGACGACGGTCAGTGATAACACCCGCATTCGTAAGCTGGCCGATGATGA
TAACGATACGGCGCAGAGTATTAACACGCGTACTGGTCGTCCGTTTATGACTATCAATGCGGCGCTGAGCGGACGCGGTG
AGCAGGGGCCGCTGGTGCAAGAGGTCAATCAGAAGCTGACGGATCTCTATCACTATCTCGATCAGATCGTTAACGCGACC
GATCCGGGACAGGCGGCGTTGAAAGCGGTGCAGGCACGGCAGGGGAATAAATTTGCCGATCCGGTATTCGCCTTACAGCA
ATACGCCCGTAGCCTTCCTGCGCCGCTGGATCGCTGGGTAGGGCAATTAGCCGGAGAGAGCGCGAGTCTGGTGACCGGGT
TGGCGATGTCGTCGCTGAATCAGGAATGGCTGGATAAGGTCGTGACGCCATTCAATGAGAAGCTGGCGGATCGTTACCCC
TTCGACCCGTCATCGAACAAGGATGTGCCGCTATCGGAAATGGAAATGTTCTTCATGGCCGGCGGGACGTTGGACAGCTT
CTATCAAACCAACCTCAAGGCGATGATGGAGAGCGGCATGCTGGAAGAAGGCATGGCATCCCCTCTGCAGGCAGAGCTGG
TGAAACAGCTTGAACGCGCTACCCGCATCCGCCAGACCCTGTTTAATGCGCAGGGCAGCCTGGAAGTACATTTCGTTCTG
GAACCGCTGGAACTGACGGCGAACAAGCGCCGCAGCGTGCTGAATCTGGACGGACAGCTGCTGGAATACAGTCACGGTCG
TCGCCAGAAAACGCCGTTGGTGTGGCCAAACAGTATGCGCGACGGGGCTGAGAGCAAGCTGACGCTGGTGCCGGACGATC
GTGAGCGTTCACCGCGCAGCCTGAGCTTTAGCGGGCCGTGGGCGATGTTCCGCTTAATCAATAGCGATCAGCTGACTCAG
GTGAATGAGAACACCTTTGACGTGCGCTTCTCGCTGGAAAATGGCGCGATGACGTATCGCGTGTATACCGATGCCAGCCA
TAACCCGTTTGCCGGTGGTCTGTTCAGCCAGTTCACGCTGCCTGACTCGCTTTATTAA