SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP014891
Component protein type	TssL
T6SS ID (Type)	T6SS01170 (Type i1)
Strain	Pectobacterium brasiliense 1692
Replicon	chromosome
Sequence	Protein sequence (259 a.a.); Nucleotide sequence (777 bp)
Reference	[1] Bellieny-Rabelo D, Tanui CK, Miguel N, et al. Transcriptome and Comparative Genomics Analyses Reveal New Functional Insights on Key Determinants of Pathogenesis and Interbacterial Competition in Pectobacterium and Dickeya spp. Appl Environ Microbiol. 2019 Jan 9;85(2). pii: e02050-18. doi: 10.1128/AEM.02050-18. Print 2019 Jan 15. PMID: 30413477

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	GT391_RS12315
Coordinate (Strand)	2757683..2758459 (+)
NCBI ID	WP_010282512.1
RefSeq	NZ_CP047495
Uniprot ID	A0A3S0XSG8_9GAMM, A0A3S1A9A0_9GAMM, A0A419AJ62_PECCA
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	2.1e-64	39..240

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-217	MSIEVIKNDQLGDLLFDHARQLDMDSDYWFRLRGQSINPMVDAVTPLLGMVERVRQLSAYEGVEDLYQRVVSEIQAIEQELHSHGYENGVILSFRYILCTFIDEAVMGREWGGQSMWSAHSLLTRFHNETWGGEKVFVLLEKLLDDPTRYRDILEFIYLCLCLGFEGRYRVMTQGREELNRVVNKLHDTLRPEPANSPTVFHLNLGQQASRYQLRRQ
Transmembrane helix	218-240	VSLRTLFVGVCVVLVAAFGLYRY
Outside	241-258	QLTHQTQDVLRQLGELLQ

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9851	-	-

Protein sequence: 259 a.a.

						>T6CP014891 NZ_CP047495:2757683-2758459 [Pectobacterium brasiliense] [TssL]
MSIEVIKNDQLGDLLFDHARQLDMDSDYWFRLRGQSINPMVDAVTPLLGMVERVRQLSAYEGVEDLYQRVVSEIQAIEQE
LHSHGYENGVILSFRYILCTFIDEAVMGREWGGQSMWSAHSLLTRFHNETWGGEKVFVLLEKLLDDPTRYRDILEFIYLC
LCLGFEGRYRVMTQGREELNRVVNKLHDTLRPEPANSPTVFHLNLGQQASRYQLRRQVSLRTLFVGVCVVLVAAFGLYRY
QLTHQTQDVLRQLGELLQ*					

Nucleotide sequence: 777 bp

						>T6CP014891 NZ_CP047495:2757683-2758459 [Pectobacterium brasiliense] [TssL]
ATGAGCATCGAGGTTATTAAAAACGATCAACTGGGCGATCTGCTTTTTGACCACGCCCGCCAGCTGGATATGGACTCTGA
CTACTGGTTCCGCCTGCGTGGACAAAGCATTAATCCGATGGTGGATGCGGTCACGCCGCTGCTGGGCATGGTAGAGCGGG
TGCGCCAGCTCTCTGCCTACGAGGGTGTGGAAGATTTGTATCAGCGCGTGGTGTCTGAAATTCAGGCTATCGAGCAGGAA
TTACACTCTCATGGCTATGAAAACGGCGTGATCTTGTCGTTCCGCTACATCCTCTGCACCTTCATTGATGAAGCTGTGAT
GGGGCGGGAGTGGGGCGGCCAAAGCATGTGGTCGGCCCATTCTCTGCTGACCCGTTTTCACAATGAGACCTGGGGCGGCG
AAAAGGTGTTTGTCCTGCTGGAGAAGCTGCTGGACGACCCCACCCGCTATCGCGACATTCTGGAATTTATCTACCTTTGC
CTCTGTCTGGGTTTTGAAGGGCGCTATCGGGTGATGACGCAGGGGCGTGAAGAACTGAATCGCGTCGTGAACAAGCTGCA
TGACACCCTGCGCCCTGAACCCGCGAATTCGCCGACGGTCTTCCACCTGAATCTGGGGCAGCAAGCCTCGCGCTACCAGC
TGCGCAGACAGGTGTCATTACGCACGCTATTTGTCGGCGTGTGTGTGGTCTTGGTGGCCGCCTTCGGCCTGTACCGCTAT
CAGCTAACTCATCAAACCCAGGACGTACTGCGTCAGCTGGGAGAATTATTACAATAA					