SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP014889
Component protein type	TssJ
T6SS ID (Type)	T6SS01170 (Type i1)
Strain	Pectobacterium brasiliense 1692
Replicon	chromosome
Sequence	Protein sequence (174 a.a.); Nucleotide sequence (522 bp)
Reference	[1] Bellieny-Rabelo D, Tanui CK, Miguel N, et al. Transcriptome and Comparative Genomics Analyses Reveal New Functional Insights on Key Determinants of Pathogenesis and Interbacterial Competition in Pectobacterium and Dickeya spp. Appl Environ Microbiol. 2019 Jan 9;85(2). pii: e02050-18. doi: 10.1128/AEM.02050-18. Print 2019 Jan 15. PMID: 30413477

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	GT391_RS12305
Coordinate (Strand)	2755806..2756327 (+)
NCBI ID	WP_010282515.1
RefSeq	NZ_CP047495
Uniprot ID	A0A086EV62_9GAMM
KEGG ID	pbra:B5S52_05495
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	2.6e-30	43..161

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-173	MLRALCLCSLMFLLSGCTTLGKMAQVAANPDIQVGSNNHQPSTVGFSLLAEPDVNPNDSGEAAPIEFQLVLLAEDSRLLATDYDQITTDIEKALAKNYINHQDYTLLPGQFKYLPPEALDEKVHYLGVVARYADSESAEWRKVIKLKNTGQTYQILVHLRRDEVEIKKDQEEE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9954	pos: 16-17. LSG-CT.	MLRALCLCSLMFLLSG

Protein sequence: 174 a.a.

						>T6CP014889 NZ_CP047495:2755806-2756327 [Pectobacterium brasiliense] [TssJ]
MLRALCLCSLMFLLSGCTTLGKMAQVAANPDIQVGSNNHQPSTVGFSLLAEPDVNPNDSGEAAPIEFQLVLLAEDSRLLA
TDYDQITTDIEKALAKNYINHQDYTLLPGQFKYLPPEALDEKVHYLGVVARYADSESAEWRKVIKLKNTGQTYQILVHLR
RDEVEIKKDQEEE*					

Nucleotide sequence: 522 bp

						>T6CP014889 NZ_CP047495:2755806-2756327 [Pectobacterium brasiliense] [TssJ]
ATGCTGCGGGCTCTGTGCTTATGTTCCCTGATGTTCCTGCTGTCGGGGTGTACCACGCTGGGCAAGATGGCGCAGGTAGC
GGCGAATCCCGACATTCAGGTGGGCAGTAATAATCATCAGCCTTCTACCGTGGGGTTTAGCCTGCTGGCGGAACCGGACG
TTAACCCGAACGACAGCGGTGAAGCCGCGCCTATTGAATTCCAGCTAGTGCTGTTGGCGGAAGATTCGCGCCTGTTAGCC
ACCGATTACGACCAAATCACGACGGATATCGAAAAGGCATTAGCCAAGAATTACATCAATCATCAGGACTACACGCTGCT
GCCGGGGCAGTTCAAGTACCTGCCGCCCGAAGCGCTGGATGAAAAGGTGCACTACCTCGGCGTGGTGGCGCGCTATGCCG
ATTCGGAAAGCGCAGAGTGGCGCAAAGTCATCAAGCTAAAGAATACCGGGCAGACGTATCAGATCCTCGTGCACCTGCGT
CGGGATGAAGTCGAAATCAAAAAAGACCAAGAAGAAGAATAA					