SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP014888
Component protein type	TssG
T6SS ID (Type)	T6SS01170 (Type i1)
Strain	Pectobacterium brasiliense 1692
Replicon	chromosome
Sequence	Protein sequence (333 a.a.); Nucleotide sequence (999 bp)
Reference	[1] Bellieny-Rabelo D, Tanui CK, Miguel N, et al. Transcriptome and Comparative Genomics Analyses Reveal New Functional Insights on Key Determinants of Pathogenesis and Interbacterial Competition in Pectobacterium and Dickeya spp. Appl Environ Microbiol. 2019 Jan 9;85(2). pii: e02050-18. doi: 10.1128/AEM.02050-18. Print 2019 Jan 15. PMID: 30413477

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	GT391_RS12295
Coordinate (Strand)	2753585..2754583 (+)
NCBI ID	WP_010282519.1
RefSeq	NZ_CP047495
Uniprot ID	A0A0M2EVS8_9GAMM
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	3.5e-93	18..318

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-332	MAGTDRSTLNDVTFRQDVSRFNFFQLVELLNQLEGVDLEQELDFRPEQERLRFRSTASIGFHPSDILQVGRDEAGRQELEVAFLGLHGSQSPMPGYYLEELAWEYAQGEQKLGVFLDFFHHRLLTLLHRAWRKYRYHVRFQNEGEDGFSRLMFALVGLGNDAVRDSLPVNRAKMLSYAGVLASPSRSPEVVAGLVIHCFDLDDVAVLAWQHRRVPIHEGQQNRLGRGNMMLGGDFVIGDKVNDCAGKFLLKVGNLSFSRFLSFLPNGEHFQPLVRFVSFILRDQLAWDLRLGFAEGEAKGLSLGSEQSSRLGWSSFLGQPPADPYVTICVQE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9910	-	-

Protein sequence: 333 a.a.

						>T6CP014888 NZ_CP047495:2753585-2754583 [Pectobacterium brasiliense] [TssG]
MAGTDRSTLNDVTFRQDVSRFNFFQLVELLNQLEGVDLEQELDFRPEQERLRFRSTASIGFHPSDILQVGRDEAGRQELE
VAFLGLHGSQSPMPGYYLEELAWEYAQGEQKLGVFLDFFHHRLLTLLHRAWRKYRYHVRFQNEGEDGFSRLMFALVGLGN
DAVRDSLPVNRAKMLSYAGVLASPSRSPEVVAGLVIHCFDLDDVAVLAWQHRRVPIHEGQQNRLGRGNMMLGGDFVIGDK
VNDCAGKFLLKVGNLSFSRFLSFLPNGEHFQPLVRFVSFILRDQLAWDLRLGFAEGEAKGLSLGSEQSSRLGWSSFLGQP
PADPYVTICVQE*					

Nucleotide sequence: 999 bp

						>T6CP014888 NZ_CP047495:2753585-2754583 [Pectobacterium brasiliense] [TssG]
ATGGCCGGTACAGATAGGTCAACACTCAATGATGTGACATTCCGTCAGGATGTCTCGCGCTTTAATTTTTTCCAATTGGT
GGAGCTACTCAACCAACTGGAAGGTGTGGATCTGGAACAAGAGCTGGATTTCCGCCCTGAACAGGAACGCCTGCGCTTTC
GCTCTACGGCCTCCATCGGTTTTCACCCCAGCGATATCTTGCAGGTGGGACGTGATGAAGCTGGCCGTCAGGAGCTGGAA
GTCGCGTTTCTTGGCCTGCACGGCAGTCAGTCACCGATGCCCGGTTATTACCTCGAAGAGCTGGCCTGGGAATACGCGCA
GGGCGAACAGAAGCTGGGCGTGTTTCTCGACTTCTTTCATCACCGCTTACTTACGCTGCTGCACCGCGCATGGCGCAAAT
ACCGCTATCACGTCCGCTTTCAGAATGAAGGGGAGGATGGATTCTCCCGCCTGATGTTTGCGCTGGTGGGGTTAGGGAAT
GACGCCGTACGCGACAGCCTGCCGGTTAACCGCGCCAAGATGCTGTCCTACGCCGGGGTGCTCGCCAGCCCCAGCCGCTC
CCCAGAAGTGGTCGCGGGCTTGGTTATTCACTGTTTTGACCTGGATGACGTGGCTGTCCTGGCCTGGCAGCACCGTCGTG
TGCCCATTCACGAAGGGCAGCAGAACCGCCTGGGCAGAGGAAACATGATGTTGGGCGGTGATTTTGTTATCGGCGACAAA
GTGAATGACTGCGCCGGCAAGTTTTTGCTCAAGGTCGGCAATCTCAGCTTTAGCCGCTTTCTCAGCTTCTTGCCCAACGG
CGAGCATTTTCAGCCGCTGGTGCGTTTTGTCTCTTTCATTCTTCGCGATCAGTTGGCCTGGGATCTGCGTCTGGGTTTTG
CCGAAGGCGAAGCCAAGGGCTTAAGTCTGGGCAGTGAGCAAAGCAGCCGGTTGGGATGGAGCAGCTTTCTCGGGCAGCCG
CCCGCCGATCCCTATGTGACGATTTGTGTGCAGGAGTAA					