SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP014887
Component protein type	TssF
T6SS ID (Type)	T6SS01170 (Type i1)
Strain	Pectobacterium brasiliense 1692
Replicon	chromosome
Sequence	Protein sequence (589 a.a.); Nucleotide sequence (1767 bp)
Reference	[1] Bellieny-Rabelo D, Tanui CK, Miguel N, et al. Transcriptome and Comparative Genomics Analyses Reveal New Functional Insights on Key Determinants of Pathogenesis and Interbacterial Competition in Pectobacterium and Dickeya spp. Appl Environ Microbiol. 2019 Jan 9;85(2). pii: e02050-18. doi: 10.1128/AEM.02050-18. Print 2019 Jan 15. PMID: 30413477

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	GT391_RS12290
Coordinate (Strand)	2751855..2753621 (+)
NCBI ID	WP_010282521.1
RefSeq	NZ_CP047495
Uniprot ID	A0A6I6X5M0_9GAMM
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	2.6e-153	3..584

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-588	MSLEHFFRDELTYLRLQGREFAKAHPELTRFLSEQTTDPDVERLLEGFAFLTGSLRAKIEDEFPELTHGLLGMLWPNYLRPVPSMTIMQFSVLPGAIAQPAFVERGCELDSLPIDDVVCHFQTCHDAWIYPADIREIKVQSGNDLSTITLDIGLHGPLSLSDLQLDKLRFYLGGDTYTAYELYFWIASQLSHIELEVDGQRFRQEASVLKTVGFEREDALLPYPGNVYSGYRILQEYFCFPESFLFFQLAGAVWPELPLTVTEFRLHFCFDRPLPAELKIRPDSFMLNCVPAINLFQHDSEPINLSGRQTDYPLKASYRNADSFEIFSVDKVEGWVEGNSGRARGIPRTYQPFESFQHQIERAKGRLALYYRIRVREAVSGNGFDHMLSFVRGDEQEVIDLDESISVTLTCTNRSRAAKLPVGSICVPTGNSPSFATFRNLVRPTRPLRPAMDGSLHWTLISNLSLNYVSLLRRDALVQILRTYDFPALHDKQAEQASRKRLAGIESIETTPIDRLVQGMPVRGLKSILSVRQSAFSSEGELYLFSTVLAHFFSLYASVNAFHLLEVVNIDNKERYRWPVQIGQHSMM

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9879	-	-

Protein sequence: 589 a.a.

						>T6CP014887 NZ_CP047495:2751855-2753621 [Pectobacterium brasiliense] [TssF]
MSLEHFFRDELTYLRLQGREFAKAHPELTRFLSEQTTDPDVERLLEGFAFLTGSLRAKIEDEFPELTHGLLGMLWPNYLR
PVPSMTIMQFSVLPGAIAQPAFVERGCELDSLPIDDVVCHFQTCHDAWIYPADIREIKVQSGNDLSTITLDIGLHGPLSL
SDLQLDKLRFYLGGDTYTAYELYFWIASQLSHIELEVDGQRFRQEASVLKTVGFEREDALLPYPGNVYSGYRILQEYFCF
PESFLFFQLAGAVWPELPLTVTEFRLHFCFDRPLPAELKIRPDSFMLNCVPAINLFQHDSEPINLSGRQTDYPLKASYRN
ADSFEIFSVDKVEGWVEGNSGRARGIPRTYQPFESFQHQIERAKGRLALYYRIRVREAVSGNGFDHMLSFVRGDEQEVID
LDESISVTLTCTNRSRAAKLPVGSICVPTGNSPSFATFRNLVRPTRPLRPAMDGSLHWTLISNLSLNYVSLLRRDALVQI
LRTYDFPALHDKQAEQASRKRLAGIESIETTPIDRLVQGMPVRGLKSILSVRQSAFSSEGELYLFSTVLAHFFSLYASVN
AFHLLEVVNIDNKERYRWPVQIGQHSMM*					

Nucleotide sequence: 1767 bp

						>T6CP014887 NZ_CP047495:2751855-2753621 [Pectobacterium brasiliense] [TssF]
ATGTCACTGGAACATTTTTTCAGGGATGAGCTGACCTACTTGCGCCTGCAAGGGCGTGAATTCGCCAAGGCGCACCCTGA
GCTTACCCGATTTTTGTCAGAACAAACCACGGATCCAGACGTCGAGCGTCTGCTGGAAGGGTTCGCCTTTTTGACAGGGA
GCCTGCGGGCGAAGATCGAGGATGAATTTCCGGAACTGACACACGGTCTGCTGGGCATGCTGTGGCCTAATTACCTGCGC
CCCGTACCGAGCATGACGATTATGCAGTTTTCGGTACTCCCCGGCGCGATTGCCCAGCCCGCGTTTGTTGAACGCGGCTG
TGAACTTGATAGTCTGCCGATTGATGATGTGGTGTGCCATTTTCAGACCTGCCACGATGCCTGGATTTATCCTGCGGATA
TCCGTGAGATCAAGGTACAGAGCGGCAACGATCTTTCCACCATCACGCTGGATATTGGGCTGCATGGCCCGCTATCGTTA
AGCGATCTGCAACTCGACAAGCTGCGCTTTTATCTGGGCGGTGATACCTATACGGCCTACGAACTCTATTTCTGGATTGC
CAGCCAACTGTCGCACATTGAGCTGGAAGTCGATGGTCAGCGTTTTCGTCAGGAAGCCAGCGTGCTGAAAACGGTCGGCT
TTGAGCGGGAAGATGCGCTATTGCCGTATCCCGGCAATGTCTATTCCGGCTACCGCATTTTGCAGGAATACTTTTGTTTT
CCTGAAAGTTTTCTCTTTTTCCAACTCGCAGGCGCGGTATGGCCGGAGCTTCCGCTGACGGTAACGGAATTCCGTCTGCA
TTTTTGCTTTGACCGCCCGCTGCCGGCGGAACTGAAGATCCGTCCTGATTCGTTCATGCTCAACTGCGTGCCGGCAATCA
ATCTCTTCCAGCATGATAGCGAGCCGATCAACCTCAGCGGACGCCAGACGGATTACCCGCTGAAAGCCAGCTACCGCAAC
GCCGATAGCTTTGAAATTTTCTCTGTGGATAAGGTTGAAGGTTGGGTTGAAGGGAATTCAGGCCGTGCTAGGGGGATTCC
ACGCACCTATCAGCCTTTTGAGAGCTTTCAGCACCAAATCGAACGTGCCAAAGGACGGCTCGCGCTCTATTACCGTATTC
GGGTAAGAGAAGCGGTCAGCGGCAACGGGTTTGATCACATGCTCTCTTTTGTGCGTGGTGATGAGCAGGAAGTGATTGAT
TTGGATGAATCCATCTCGGTAACGCTGACCTGCACCAACCGATCGCGTGCGGCGAAACTGCCTGTTGGGTCGATCTGCGT
GCCAACCGGCAATTCGCCGTCTTTCGCGACGTTTCGCAATCTGGTACGGCCGACGCGACCGCTGCGGCCTGCCATGGATG
GCAGCCTTCACTGGACGCTGATCTCCAATCTGTCTCTGAACTATGTGTCGCTGCTGCGGCGCGATGCGCTGGTGCAGATC
CTGCGTACTTACGATTTCCCGGCGCTGCACGACAAGCAGGCGGAACAGGCCTCGCGTAAGCGTCTGGCAGGGATCGAATC
TATCGAAACCACACCGATCGACCGTCTGGTGCAGGGGATGCCGGTTCGCGGCTTGAAATCCATCCTGTCAGTACGGCAAT
CCGCGTTTTCCAGTGAAGGAGAACTCTATCTGTTTAGTACGGTGCTGGCGCACTTCTTCTCGCTATACGCCAGCGTCAAC
GCTTTCCACCTGTTGGAAGTGGTCAACATCGATAACAAGGAGCGCTACCGATGGCCGGTACAGATAGGTCAACACTCAAT
GATGTGA					