Detailed information of component protein
Summary
| Component ID | T6CP014884 |
| Component protein type | TssB |
| T6SS ID (Type) | T6SS01170  (Type i1) |
| Strain | Pectobacterium brasiliense 1692 |
| Replicon | chromosome |
| Sequence | Protein sequence (168 a.a.); Nucleotide sequence (504 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | GT391_RS12275 |
| Coordinate (Strand) | 2749401..2749904 (+) |
| NCBI ID | WP_010282535.1 |
| RefSeq | NZ_CP047495 |
| Uniprot ID | A0A1V2QZV7_9GAMM, A0A086EV57_9GAMM, A0A249Q8S6_9GAMM, A0A426IJD6_9GAMM, A0A221T6B9_9GAMM, A0A1M5U140_PECCA, A0A094TDZ7_9GAMM |
| KEGG ID | pbra:B5S52_05465, pcv:BCS7_16400 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS_VipA | PF05591.14 | 2.6e-54 | 9..160 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-167 | MAKGTDGASVAPKERINIKYVPATGGQQAEIELPLTLMIVGNMKGRTEETPIEERQTVSIDKNNFTSVMKEANLELNFSVPNRLEEESQDDLPVKLSIGGLNDFSPDRIAQQVPELRKLLDLREALVALKGPLGNIPSFRNRLQDLLSSEEAREQLLKELDLVKPAE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9885 | - | - |
Protein sequence: 168 a.a.
>T6CP014884 NZ_CP047495:2749401-2749904 [Pectobacterium brasiliense] [TssB]
MAKGTDGASVAPKERINIKYVPATGGQQAEIELPLTLMIVGNMKGRTEETPIEERQTVSIDKNNFTSVMKEANLELNFSV
PNRLEEESQDDLPVKLSIGGLNDFSPDRIAQQVPELRKLLDLREALVALKGPLGNIPSFRNRLQDLLSSEEAREQLLKEL
DLVKPAE*
MAKGTDGASVAPKERINIKYVPATGGQQAEIELPLTLMIVGNMKGRTEETPIEERQTVSIDKNNFTSVMKEANLELNFSV
PNRLEEESQDDLPVKLSIGGLNDFSPDRIAQQVPELRKLLDLREALVALKGPLGNIPSFRNRLQDLLSSEEAREQLLKEL
DLVKPAE*
Nucleotide sequence: 504 bp
>T6CP014884 NZ_CP047495:2749401-2749904 [Pectobacterium brasiliense] [TssB]
ATGGCAAAAGGAACTGATGGTGCATCTGTCGCACCAAAGGAACGTATTAATATCAAATATGTTCCGGCCACTGGTGGTCA
GCAGGCGGAAATAGAATTGCCGTTGACGCTGATGATCGTGGGAAATATGAAAGGGCGCACAGAGGAAACGCCGATCGAAG
AACGTCAGACCGTATCGATCGATAAGAACAACTTTACCTCAGTGATGAAAGAAGCAAATCTGGAATTGAACTTCAGTGTG
CCAAACCGCCTTGAAGAAGAAAGTCAGGATGACCTGCCAGTGAAACTGAGCATCGGCGGTCTGAATGATTTCTCCCCTGA
CCGCATTGCCCAGCAAGTGCCTGAATTACGTAAGCTGCTTGATTTACGTGAAGCACTGGTGGCGCTGAAAGGCCCCCTCG
GCAATATTCCCTCATTCCGTAATCGTTTGCAGGATCTGTTGTCCAGCGAAGAAGCCAGAGAGCAGCTTCTGAAAGAGCTC
GATCTGGTCAAACCCGCCGAATAA
ATGGCAAAAGGAACTGATGGTGCATCTGTCGCACCAAAGGAACGTATTAATATCAAATATGTTCCGGCCACTGGTGGTCA
GCAGGCGGAAATAGAATTGCCGTTGACGCTGATGATCGTGGGAAATATGAAAGGGCGCACAGAGGAAACGCCGATCGAAG
AACGTCAGACCGTATCGATCGATAAGAACAACTTTACCTCAGTGATGAAAGAAGCAAATCTGGAATTGAACTTCAGTGTG
CCAAACCGCCTTGAAGAAGAAAGTCAGGATGACCTGCCAGTGAAACTGAGCATCGGCGGTCTGAATGATTTCTCCCCTGA
CCGCATTGCCCAGCAAGTGCCTGAATTACGTAAGCTGCTTGATTTACGTGAAGCACTGGTGGCGCTGAAAGGCCCCCTCG
GCAATATTCCCTCATTCCGTAATCGTTTGCAGGATCTGTTGTCCAGCGAAGAAGCCAGAGAGCAGCTTCTGAAAGAGCTC
GATCTGGTCAAACCCGCCGAATAA