Detailed information of component protein


Summary

Component ID T6CP014865
Component protein type TssL
T6SS ID (Type) T6SS01168 experimental (Type i1)
Strain Vibrio cholerae C6706
Replicon chromosome
Sequence Protein sequence (258 a.a.); Nucleotide sequence (774 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) GPY04_RS15960
Coordinate (Strand) 306368..307141 (-)
NCBI ID WP_000083409.1
RefSeq NZ_CP046845
Uniprot ID Q9KN50_VIBCH, A0A0X1L1V7_VIBCO, A0A0H3ADB8_VIBC3, A0A0K9UV49_VIBCL, A0A7U8WPX0_VIBCL, A0A0H3Q6L3_VIBCL, C3LUD9_VIBCM, A0A023PRL8_VIBCL
KEGG ID vcm:VCM66_A0113, vcq:EN18_04300, vco:VC0395_0022, vch:VCA0115
PDB ID 4V3I
Pfam domain hit(s)

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Domain
Pfam ID
E-value
Aligned region
DotUPF09850.112.2e-6139..240
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Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

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Prediction                
Region    
Sequence
Inside1-219MSQSKKETPLASLLFDDVEKINHDQDYWFQLRGDNPNVLIDAATPLFGLSLRVRTLTECDNIEQIYRQTIEEIKAIEIELTEQGYEHAILMAYRYILCAFLDESVMGTEWGASSLWAEHSMLSRFHNETWGGEKVFTILSRLEGEPHRYQALLAFIYHCLILGFEGKYRVMEGGQAEREKVISRLHQLLSSLEESEPQDLTRPTDHVVRAKYTLSRQMP
Transmembrane helix220-242VWSVFAGFIVLWVGLFLGYSYVL
Outside243-257HSKSSDVLNQLNQIL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
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Prediction       
Probability
Cleavage site
Signal peptide sequence
Other0.9933--


  Protein sequence: 258 a.a.    

>T6CP014865 NZ_CP046845:c307141-306368 [Vibrio cholerae C6706] [TssL]
MSQSKKETPLASLLFDDVEKINHDQDYWFQLRGDNPNVLIDAATPLFGLSLRVRTLTECDNIEQIYRQTIEEIKAIEIEL
TEQGYEHAILMAYRYILCAFLDESVMGTEWGASSLWAEHSMLSRFHNETWGGEKVFTILSRLEGEPHRYQALLAFIYHCL
ILGFEGKYRVMEGGQAEREKVISRLHQLLSSLEESEPQDLTRPTDHVVRAKYTLSRQMPVWSVFAGFIVLWVGLFLGYSY
VLHSKSSDVLNQLNQIL*

  Nucleotide sequence: 774 bp    

>T6CP014865 NZ_CP046845:c307141-306368 [Vibrio cholerae C6706] [TssL]
ATGTCACAGAGTAAAAAAGAGACGCCATTAGCCAGTTTGTTGTTCGACGATGTGGAAAAAATTAACCACGACCAAGACTA
CTGGTTCCAATTGCGTGGGGACAATCCGAATGTGCTGATTGATGCCGCGACACCCTTGTTTGGGCTTTCTCTGCGGGTTC
GTACCCTCACCGAATGCGACAACATTGAACAGATCTACCGTCAGACGATTGAAGAGATCAAAGCGATTGAAATTGAGTTG
ACGGAGCAAGGTTACGAACACGCCATTTTGATGGCCTATCGCTACATCTTGTGCGCCTTTTTGGATGAATCCGTGATGGG
AACCGAGTGGGGCGCGTCCAGTTTATGGGCCGAGCACTCCATGCTGTCACGCTTCCATAATGAAACGTGGGGTGGCGAGA
AAGTGTTTACGATTTTGAGCCGTCTTGAGGGTGAACCACACCGTTACCAAGCGCTATTGGCCTTTATTTATCATTGCCTG
ATTTTGGGCTTTGAAGGCAAATACCGTGTGATGGAAGGGGGACAAGCTGAGCGCGAAAAAGTGATCAGCCGCCTGCATCA
ACTGCTGAGCAGCTTGGAAGAGAGCGAGCCACAAGACTTAACCCGTCCGACCGATCATGTGGTGCGTGCGAAATATACCC
TAAGCAGACAGATGCCTGTTTGGTCTGTGTTTGCTGGCTTTATCGTGCTTTGGGTAGGTCTATTTCTCGGCTACAGCTAT
GTACTGCATAGCAAATCCAGCGATGTGCTCAATCAACTCAATCAAATACTTTAA