Detailed information of component protein


Summary

Component ID T6CP014264
Component protein type TssL
T6SS ID (Type) T6SS01112 experimental (Type i1)
Strain Vibrio cholerae strain A1552
Replicon chromosome
Sequence Protein sequence (258 a.a.); Nucleotide sequence (774 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VCA1552_RS14760
Coordinate (Strand) 125478..126251 (+)
NCBI ID WP_000083409.1
RefSeq NZ_CP024868
Uniprot ID Q9KN50_VIBCH, A0A0X1L1V7_VIBCO, A0A0H3ADB8_VIBC3, A0A0K9UV49_VIBCL, A0A7U8WPX0_VIBCL, A0A0H3Q6L3_VIBCL, C3LUD9_VIBCM, A0A023PRL8_VIBCL
KEGG ID vcm:VCM66_A0113, vcq:EN18_04300, vco:VC0395_0022, vch:VCA0115
PDB ID 4V3I
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 2.2e-61 39..240
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-219 MSQSKKETPLASLLFDDVEKINHDQDYWFQLRGDNPNVLIDAATPLFGLSLRVRTLTECDNIEQIYRQTIEEIKAIEIELTEQGYEHAILMAYRYILCAFLDESVMGTEWGASSLWAEHSMLSRFHNETWGGEKVFTILSRLEGEPHRYQALLAFIYHCLILGFEGKYRVMEGGQAEREKVISRLHQLLSSLEESEPQDLTRPTDHVVRAKYTLSRQMP
Transmembrane helix 220-242 VWSVFAGFIVLWVGLFLGYSYVL
Outside 243-257 HSKSSDVLNQLNQIL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9933 - -


  Protein sequence: 258 a.a.    

>T6CP014264 NZ_CP024868:125478-126251 [Vibrio cholerae] [TssL]
MSQSKKETPLASLLFDDVEKINHDQDYWFQLRGDNPNVLIDAATPLFGLSLRVRTLTECDNIEQIYRQTIEEIKAIEIEL
TEQGYEHAILMAYRYILCAFLDESVMGTEWGASSLWAEHSMLSRFHNETWGGEKVFTILSRLEGEPHRYQALLAFIYHCL
ILGFEGKYRVMEGGQAEREKVISRLHQLLSSLEESEPQDLTRPTDHVVRAKYTLSRQMPVWSVFAGFIVLWVGLFLGYSY
VLHSKSSDVLNQLNQIL*

  Nucleotide sequence: 774 bp    

>T6CP014264 NZ_CP024868:125478-126251 [Vibrio cholerae] [TssL]
ATGTCACAGAGTAAAAAAGAGACGCCATTAGCCAGTTTGTTGTTCGACGATGTGGAAAAAATTAACCACGACCAAGACTA
CTGGTTCCAATTGCGTGGGGACAATCCGAATGTGCTGATTGATGCCGCGACACCCTTGTTTGGGCTTTCTCTGCGGGTTC
GTACCCTCACCGAATGCGACAACATTGAACAGATCTACCGTCAGACGATTGAAGAGATCAAAGCGATTGAAATTGAGTTG
ACGGAGCAAGGTTACGAACACGCCATTTTGATGGCCTATCGCTACATCTTGTGCGCCTTTTTGGATGAATCCGTGATGGG
AACCGAGTGGGGCGCGTCCAGTTTATGGGCCGAGCACTCCATGCTGTCACGCTTCCATAATGAAACGTGGGGTGGCGAGA
AAGTGTTTACGATTTTGAGCCGTCTTGAGGGTGAACCACACCGTTACCAAGCGCTATTGGCCTTTATTTATCATTGCCTG
ATTTTGGGCTTTGAAGGCAAATACCGTGTGATGGAAGGGGGACAAGCTGAGCGCGAAAAAGTGATCAGCCGCCTGCATCA
ACTGCTGAGCAGCTTGGAAGAGAGCGAGCCACAAGACTTAACCCGTCCGACCGATCATGTGGTGCGTGCGAAATATACCC
TAAGCAGACAGATGCCTGTTTGGTCTGTGTTTGCTGGCTTTATCGTGCTTTGGGTAGGTCTATTTCTCGGCTACAGCTAT
GTACTGCATAGCAAATCCAGCGATGTGCTCAATCAACTCAATCAAATACTTTAA