Detailed information of component protein
Summary
| Component ID | T6CP014006 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS01088  (Type i1) |
| Strain | Vibrio anguillarum strain MHK3 |
| Replicon | chromosome |
| Sequence | Protein sequence (160 a.a.); Nucleotide sequence (480 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | CG015_RS14350 |
| Coordinate (Strand) | 256296..256775 (-) |
| NCBI ID | WP_013868298.1 |
| RefSeq | NZ_CP022469 |
| Uniprot ID | A0A233IFX2_9GAMM, A0A233HNE7_9VIBR, A0A6B2BPI4_9VIBR, A0A223N1R5_9VIBR, A0A7Y3QV81_9VIBR, B6SDI5_VIBAN, F7YT75_VIBA7, A0A7U4CV87_VIBAN |
| KEGG ID | van:VAA_02840, vqi:CCZ37_13765 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 3.1e-28 | 31..145 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-159 | VNKRSFVLLMLLTVLTACSSSEKYEPATQPTKVTFSLVSDEGTNPNIWGEASPIEVQVFELKDDSMFMSADYDQVKADYKKALRSNFIANYDYVMTPGQFKFVNAFEIDEGTNYIGVMAHFAEPELSEWKKAVKVLNKGREYHLLMYFKDYDVKLERVE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9991 | pos: 17-18. LTA-CS. | VNKRSFVLLMLLTVLTA |
Protein sequence: 160 a.a.
>T6CP014006 NZ_CP022469:c256775-256296 [Vibrio anguillarum] [TssJ]
VNKRSFVLLMLLTVLTACSSSEKYEPATQPTKVTFSLVSDEGTNPNIWGEASPIEVQVFELKDDSMFMSADYDQVKADYK
KALRSNFIANYDYVMTPGQFKFVNAFEIDEGTNYIGVMAHFAEPELSEWKKAVKVLNKGREYHLLMYFKDYDVKLERVE*
VNKRSFVLLMLLTVLTACSSSEKYEPATQPTKVTFSLVSDEGTNPNIWGEASPIEVQVFELKDDSMFMSADYDQVKADYK
KALRSNFIANYDYVMTPGQFKFVNAFEIDEGTNYIGVMAHFAEPELSEWKKAVKVLNKGREYHLLMYFKDYDVKLERVE*
Nucleotide sequence: 480 bp
>T6CP014006 NZ_CP022469:c256775-256296 [Vibrio anguillarum] [TssJ]
GTGAACAAGCGGTCTTTTGTCCTGCTGATGCTACTGACGGTATTAACCGCCTGTAGCTCATCAGAAAAGTATGAGCCAGC
CACACAGCCGACCAAGGTAACGTTCAGTCTCGTTAGTGATGAAGGTACTAACCCTAATATTTGGGGTGAAGCGTCGCCGA
TAGAGGTTCAAGTGTTTGAGCTCAAAGATGATTCGATGTTTATGTCTGCCGATTATGACCAAGTAAAAGCAGACTACAAA
AAAGCGTTACGCAGCAATTTTATCGCCAATTACGATTATGTAATGACACCAGGCCAGTTTAAGTTTGTGAACGCGTTCGA
GATTGATGAAGGCACGAACTACATTGGGGTCATGGCGCATTTTGCTGAACCTGAGCTGAGTGAATGGAAAAAGGCCGTAA
AAGTGCTTAACAAAGGTAGGGAATACCATCTTTTGATGTATTTCAAAGACTACGATGTAAAACTGGAAAGGGTGGAATAA
GTGAACAAGCGGTCTTTTGTCCTGCTGATGCTACTGACGGTATTAACCGCCTGTAGCTCATCAGAAAAGTATGAGCCAGC
CACACAGCCGACCAAGGTAACGTTCAGTCTCGTTAGTGATGAAGGTACTAACCCTAATATTTGGGGTGAAGCGTCGCCGA
TAGAGGTTCAAGTGTTTGAGCTCAAAGATGATTCGATGTTTATGTCTGCCGATTATGACCAAGTAAAAGCAGACTACAAA
AAAGCGTTACGCAGCAATTTTATCGCCAATTACGATTATGTAATGACACCAGGCCAGTTTAAGTTTGTGAACGCGTTCGA
GATTGATGAAGGCACGAACTACATTGGGGTCATGGCGCATTTTGCTGAACCTGAGCTGAGTGAATGGAAAAAGGCCGTAA
AAGTGCTTAACAAAGGTAGGGAATACCATCTTTTGATGTATTTCAAAGACTACGATGTAAAACTGGAAAGGGTGGAATAA