Detailed information of component protein
Summary
| Component ID | T6CP014004 |
| Component protein type | TssL |
| T6SS ID (Type) | T6SS01088  (Type i1) |
| Strain | Vibrio anguillarum strain MHK3 |
| Replicon | chromosome |
| Sequence | Protein sequence (258 a.a.); Nucleotide sequence (774 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | CG015_RS14340 |
| Coordinate (Strand) | 254181..254954 (-) |
| NCBI ID | WP_026027751.1 |
| RefSeq | NZ_CP022469 |
| Uniprot ID | A0A233IF24_9GAMM, A0A233HP09_9VIBR, A0A6B2BZ90_9VIBR, A0A7Y3QVB3_9VIBR |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DotU | PF09850.11 | 5e-62 | 39..240 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-219 | MAIDKKNTQYSGLLFDSVEQINHDQDYWFQLRGDNPNVLIDAATPLFGLSLRVRSLSECDNIEQIYKQTVEEIKAIEIELAEQGFEHAILMAYRYILCAFLDEAVMGTEWGASSVWAEHSMLSRFHSETWGGEKVFTILSRLEGEPKRYRALLEFIYQCLVLGFEGKYRVMESGHNEREKVISRLHDTVSSLDENEPKELTSATDHVVKSKYKLSRQLP |
| Transmembrane helix | 220-242 | VWSVLLGFVIIWVGVFLGYSYLL |
| Outside | 243-257 | HSQSADVLNQLNQIL |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9948 | - | - |
Protein sequence: 258 a.a.
>T6CP014004 NZ_CP022469:c254954-254181 [Vibrio anguillarum] [TssL]
MAIDKKNTQYSGLLFDSVEQINHDQDYWFQLRGDNPNVLIDAATPLFGLSLRVRSLSECDNIEQIYKQTVEEIKAIEIEL
AEQGFEHAILMAYRYILCAFLDEAVMGTEWGASSVWAEHSMLSRFHSETWGGEKVFTILSRLEGEPKRYRALLEFIYQCL
VLGFEGKYRVMESGHNEREKVISRLHDTVSSLDENEPKELTSATDHVVKSKYKLSRQLPVWSVLLGFVIIWVGVFLGYSY
LLHSQSADVLNQLNQIL*
MAIDKKNTQYSGLLFDSVEQINHDQDYWFQLRGDNPNVLIDAATPLFGLSLRVRSLSECDNIEQIYKQTVEEIKAIEIEL
AEQGFEHAILMAYRYILCAFLDEAVMGTEWGASSVWAEHSMLSRFHSETWGGEKVFTILSRLEGEPKRYRALLEFIYQCL
VLGFEGKYRVMESGHNEREKVISRLHDTVSSLDENEPKELTSATDHVVKSKYKLSRQLPVWSVLLGFVIIWVGVFLGYSY
LLHSQSADVLNQLNQIL*
Nucleotide sequence: 774 bp
>T6CP014004 NZ_CP022469:c254954-254181 [Vibrio anguillarum] [TssL]
ATGGCCATTGATAAAAAAAACACCCAATACAGCGGTCTGCTTTTTGACAGCGTCGAGCAGATCAACCACGACCAAGATTA
CTGGTTTCAACTGCGTGGTGATAACCCTAATGTTTTGATTGATGCTGCTACCCCTCTGTTTGGTTTGTCGCTGCGGGTAC
GCAGCTTGTCAGAGTGCGACAACATTGAGCAGATCTACAAACAGACGGTAGAAGAGATTAAAGCGATTGAAATCGAGCTT
GCCGAGCAAGGGTTTGAGCACGCGATTTTGATGGCTTATCGCTACATTCTGTGTGCGTTTTTGGATGAAGCCGTCATGGG
AACCGAGTGGGGAGCATCCAGTGTGTGGGCTGAACATTCTATGCTGTCTCGTTTCCACAGTGAGACTTGGGGCGGCGAAA
AAGTGTTTACCATCTTAAGTCGCTTGGAAGGTGAACCTAAACGTTATCGCGCGTTGCTAGAGTTTATCTATCAATGCCTA
GTGTTAGGTTTTGAAGGTAAATACCGTGTGATGGAAAGCGGTCACAATGAGCGTGAAAAGGTGATCAGTCGTTTACATGA
CACCGTCAGTTCGTTGGATGAAAACGAGCCGAAGGAACTGACCTCCGCCACGGACCATGTGGTGAAATCAAAATATAAGT
TAAGCAGGCAGTTACCGGTTTGGTCAGTACTGCTTGGTTTTGTCATTATTTGGGTCGGTGTGTTTTTAGGCTATTCCTAT
TTACTGCACAGCCAATCGGCAGACGTATTAAACCAATTGAACCAAATACTCTAA
ATGGCCATTGATAAAAAAAACACCCAATACAGCGGTCTGCTTTTTGACAGCGTCGAGCAGATCAACCACGACCAAGATTA
CTGGTTTCAACTGCGTGGTGATAACCCTAATGTTTTGATTGATGCTGCTACCCCTCTGTTTGGTTTGTCGCTGCGGGTAC
GCAGCTTGTCAGAGTGCGACAACATTGAGCAGATCTACAAACAGACGGTAGAAGAGATTAAAGCGATTGAAATCGAGCTT
GCCGAGCAAGGGTTTGAGCACGCGATTTTGATGGCTTATCGCTACATTCTGTGTGCGTTTTTGGATGAAGCCGTCATGGG
AACCGAGTGGGGAGCATCCAGTGTGTGGGCTGAACATTCTATGCTGTCTCGTTTCCACAGTGAGACTTGGGGCGGCGAAA
AAGTGTTTACCATCTTAAGTCGCTTGGAAGGTGAACCTAAACGTTATCGCGCGTTGCTAGAGTTTATCTATCAATGCCTA
GTGTTAGGTTTTGAAGGTAAATACCGTGTGATGGAAAGCGGTCACAATGAGCGTGAAAAGGTGATCAGTCGTTTACATGA
CACCGTCAGTTCGTTGGATGAAAACGAGCCGAAGGAACTGACCTCCGCCACGGACCATGTGGTGAAATCAAAATATAAGT
TAAGCAGGCAGTTACCGGTTTGGTCAGTACTGCTTGGTTTTGTCATTATTTGGGTCGGTGTGTTTTTAGGCTATTCCTAT
TTACTGCACAGCCAATCGGCAGACGTATTAAACCAATTGAACCAAATACTCTAA