Detailed information of component protein
Summary
Component ID | T6CP014001 |
Component protein type | TssM |
T6SS ID (Type) | T6SS01088 (Type i1) |
Strain | Vibrio anguillarum strain MHK3 |
Replicon | chromosome |
Sequence | Protein sequence (1167 a.a.); Nucleotide sequence (3501 bp) |
Reference |
External database links
Locus tag (Gene) | CG015_RS14315 |
Coordinate (Strand) | 244348..247848 (-) |
NCBI ID | WP_026028432.1 |
RefSeq | NZ_CP022469 |
Uniprot ID | UPI000474C53C |
KEGG ID | - |
PDB ID | - |
Pfam domain hit(s)
Domain | Pfam ID | E-value | Aligned region |
---|---|---|---|
IcmF-related_N | PF14331.8 | 7.9e-76 | 188..445 |
IcmF-related | PF06761.14 | 1.7e-71 | 497..809 |
IcmF_C | PF06744.14 | 2.5e-23 | 1039..1145 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
Prediction | Region | Sequence |
---|---|---|
Inside | 1-6 | IAAAMP |
Transmembrane helix | 7-24 | ILLFSTFILLNVAIWWAG |
Outside | 25-36 | PWLEIAGNKPLE |
Transmembrane helix | 37-59 | SITARAVASSLFTLGALAVWGVW |
Inside | 60-437 | QWRKLQGFKAEQKREDQLRQDPIKAYEERQEVELNQVMVSMKENLNKRNYLYALPWYLVLGLENAGKTSLINRSGQNFVFSSVMRASGQKSENPYSFDWWIGDESVLIDPDGELLTQGNHSNDNDGAMERRLWLHFVNWLERTRSRRPLNGIVLALDVSHLATATATERKAYANLLRARLRELMETLSTRLPVYIALTKLDLLHGFEPFFKHYSRSQREDVLGFTFSLDSIDDLDNWLNEFSKDYSQFVERINDVLPHAVSVPMDLEERNAIYSFTRQISGLKEILLQFFNDALASDQFSTSALVRGAYFTSVYQQGVPTNAFDDAASRRYGLDHAINKAQQAKNSTVYFTQKLFNNIIYPEAGLASDNFRVAKQKRR |
Transmembrane helix | 438-457 | LIGLSVVACSIATVLLVGTW |
Outside | 458-1166 | HRYYLSNVKHSDAVLAKVNEYKNEFPSNLYLASQQDVLVPLNKIREATLEFGFFRDKPQYISDFGLYQGHTIGPMVEATYLNLLENRFLPLLMADVVVQLNQAKTDEEKLAVLRVYRMMVDKSGRYKDYVLDYFSKYWQQEFAGQRVIQEELLGHLDYAMLHTDLAGDRARGDRNAESVMKPYDQVVAKVQSDLGSMPNDQRVYRNLKLNAQTVLGPSINLRNLIGPVFDVVFEERVVNSSSLYIPQMLTKKGFEDYFMPQSESVSELALIDSWVLGQSKSAQFSEADKHDLRNKIRSLYVADYTNTWRAALNEISVKYFSDINDAVSVLENFTGNIEPMQRLLRTLESNTQMYASVPKDEAAQKELLKNPKYKVASQIDAPFAELNAMLKPVGDKPAYINEVLVAVEELQNYLKSIQEAPDVGMAALDATKARVKLVNADPIYTVKRIASGLPKPLDTMVSKLADESWYVVKQEAIKHLEVRWHDDVYKTFEEKLAGRYPFNESSNKDSSLSDFEAFFAPNGTLDNFYNNQLRMFVEENIAVNDDDNAQSIIRKDVLEQLKQAKKIQQAFFNRKGVLDVSFSVEPLRLSNNKRRSVLNVDGQYLAYSHGPRDSVELIWPNTLRDSAISKVTLVPTKNNMSPRSINIQGPWAFFRLLEQGDVVGASSTSVDFKFAVDGGEMIYRLNSEADINPFTERLFKSFKLSKTLY |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction | Probability | Cleavage site | Signal peptide sequence |
---|---|---|---|
Other | 0.9967 | - | - |
Protein sequence: 1167 a.a.
>T6CP014001 NZ_CP022469:c247848-244348 [Vibrio anguillarum] [TssM]
IAAAMPILLFSTFILLNVAIWWAGPWLEIAGNKPLESITARAVASSLFTLGALAVWGVWQWRKLQGFKAEQKREDQLRQD
PIKAYEERQEVELNQVMVSMKENLNKRNYLYALPWYLVLGLENAGKTSLINRSGQNFVFSSVMRASGQKSENPYSFDWWI
GDESVLIDPDGELLTQGNHSNDNDGAMERRLWLHFVNWLERTRSRRPLNGIVLALDVSHLATATATERKAYANLLRARLR
ELMETLSTRLPVYIALTKLDLLHGFEPFFKHYSRSQREDVLGFTFSLDSIDDLDNWLNEFSKDYSQFVERINDVLPHAVS
VPMDLEERNAIYSFTRQISGLKEILLQFFNDALASDQFSTSALVRGAYFTSVYQQGVPTNAFDDAASRRYGLDHAINKAQ
QAKNSTVYFTQKLFNNIIYPEAGLASDNFRVAKQKRRLIGLSVVACSIATVLLVGTWHRYYLSNVKHSDAVLAKVNEYKN
EFPSNLYLASQQDVLVPLNKIREATLEFGFFRDKPQYISDFGLYQGHTIGPMVEATYLNLLENRFLPLLMADVVVQLNQA
KTDEEKLAVLRVYRMMVDKSGRYKDYVLDYFSKYWQQEFAGQRVIQEELLGHLDYAMLHTDLAGDRARGDRNAESVMKPY
DQVVAKVQSDLGSMPNDQRVYRNLKLNAQTVLGPSINLRNLIGPVFDVVFEERVVNSSSLYIPQMLTKKGFEDYFMPQSE
SVSELALIDSWVLGQSKSAQFSEADKHDLRNKIRSLYVADYTNTWRAALNEISVKYFSDINDAVSVLENFTGNIEPMQRL
LRTLESNTQMYASVPKDEAAQKELLKNPKYKVASQIDAPFAELNAMLKPVGDKPAYINEVLVAVEELQNYLKSIQEAPDV
GMAALDATKARVKLVNADPIYTVKRIASGLPKPLDTMVSKLADESWYVVKQEAIKHLEVRWHDDVYKTFEEKLAGRYPFN
ESSNKDSSLSDFEAFFAPNGTLDNFYNNQLRMFVEENIAVNDDDNAQSIIRKDVLEQLKQAKKIQQAFFNRKGVLDVSFS
VEPLRLSNNKRRSVLNVDGQYLAYSHGPRDSVELIWPNTLRDSAISKVTLVPTKNNMSPRSINIQGPWAFFRLLEQGDVV
GASSTSVDFKFAVDGGEMIYRLNSEADINPFTERLFKSFKLSKTLY*
IAAAMPILLFSTFILLNVAIWWAGPWLEIAGNKPLESITARAVASSLFTLGALAVWGVWQWRKLQGFKAEQKREDQLRQD
PIKAYEERQEVELNQVMVSMKENLNKRNYLYALPWYLVLGLENAGKTSLINRSGQNFVFSSVMRASGQKSENPYSFDWWI
GDESVLIDPDGELLTQGNHSNDNDGAMERRLWLHFVNWLERTRSRRPLNGIVLALDVSHLATATATERKAYANLLRARLR
ELMETLSTRLPVYIALTKLDLLHGFEPFFKHYSRSQREDVLGFTFSLDSIDDLDNWLNEFSKDYSQFVERINDVLPHAVS
VPMDLEERNAIYSFTRQISGLKEILLQFFNDALASDQFSTSALVRGAYFTSVYQQGVPTNAFDDAASRRYGLDHAINKAQ
QAKNSTVYFTQKLFNNIIYPEAGLASDNFRVAKQKRRLIGLSVVACSIATVLLVGTWHRYYLSNVKHSDAVLAKVNEYKN
EFPSNLYLASQQDVLVPLNKIREATLEFGFFRDKPQYISDFGLYQGHTIGPMVEATYLNLLENRFLPLLMADVVVQLNQA
KTDEEKLAVLRVYRMMVDKSGRYKDYVLDYFSKYWQQEFAGQRVIQEELLGHLDYAMLHTDLAGDRARGDRNAESVMKPY
DQVVAKVQSDLGSMPNDQRVYRNLKLNAQTVLGPSINLRNLIGPVFDVVFEERVVNSSSLYIPQMLTKKGFEDYFMPQSE
SVSELALIDSWVLGQSKSAQFSEADKHDLRNKIRSLYVADYTNTWRAALNEISVKYFSDINDAVSVLENFTGNIEPMQRL
LRTLESNTQMYASVPKDEAAQKELLKNPKYKVASQIDAPFAELNAMLKPVGDKPAYINEVLVAVEELQNYLKSIQEAPDV
GMAALDATKARVKLVNADPIYTVKRIASGLPKPLDTMVSKLADESWYVVKQEAIKHLEVRWHDDVYKTFEEKLAGRYPFN
ESSNKDSSLSDFEAFFAPNGTLDNFYNNQLRMFVEENIAVNDDDNAQSIIRKDVLEQLKQAKKIQQAFFNRKGVLDVSFS
VEPLRLSNNKRRSVLNVDGQYLAYSHGPRDSVELIWPNTLRDSAISKVTLVPTKNNMSPRSINIQGPWAFFRLLEQGDVV
GASSTSVDFKFAVDGGEMIYRLNSEADINPFTERLFKSFKLSKTLY*
Nucleotide sequence: 3501 bp
>T6CP014001 NZ_CP022469:c247848-244348 [Vibrio anguillarum] [TssM]
ATTGCCGCGGCGATGCCTATTTTGCTATTTAGCACATTCATTCTATTGAATGTTGCGATTTGGTGGGCAGGCCCTTGGCT
AGAGATCGCGGGTAATAAGCCACTTGAATCGATAACCGCTCGCGCAGTGGCAAGTAGCTTGTTCACTTTAGGTGCATTGG
CCGTTTGGGGTGTTTGGCAGTGGCGAAAATTGCAAGGTTTTAAAGCCGAGCAAAAGCGTGAAGATCAGCTTCGCCAAGAT
CCAATTAAAGCCTATGAAGAGCGCCAAGAGGTTGAGCTGAACCAAGTGATGGTCAGCATGAAAGAGAACCTCAATAAACG
TAATTATCTTTATGCGTTGCCATGGTATTTAGTGCTCGGTTTGGAAAATGCCGGTAAAACCAGCCTCATCAACCGCTCTG
GGCAAAACTTTGTTTTTTCTTCGGTAATGCGAGCCTCGGGTCAAAAAAGTGAAAACCCGTATTCGTTTGATTGGTGGATT
GGCGATGAATCGGTATTGATTGATCCAGATGGAGAGCTACTGACACAAGGTAACCATAGCAATGATAACGATGGTGCGAT
GGAGCGCCGTTTGTGGCTTCATTTTGTTAATTGGTTGGAGAGAACGCGTAGCCGTCGTCCACTCAACGGTATTGTTTTAG
CGTTAGACGTTTCTCACTTAGCAACCGCAACTGCAACCGAGCGTAAAGCTTACGCCAATTTATTACGTGCTCGCTTACGT
GAACTGATGGAGACGTTATCCACGCGTCTACCTGTCTATATTGCATTGACGAAATTGGATTTATTACACGGTTTTGAACC
TTTCTTTAAACATTACTCGCGCAGTCAGCGTGAAGATGTGCTGGGCTTTACCTTTTCGCTTGACTCTATTGATGACTTAG
ATAATTGGCTCAACGAGTTTTCAAAAGACTACAGTCAGTTTGTTGAGCGCATTAATGATGTACTGCCGCATGCGGTTTCT
GTACCTATGGATCTCGAAGAGCGCAATGCTATTTATAGTTTCACGCGTCAAATTTCTGGTTTAAAAGAGATCTTATTACA
ATTTTTCAACGATGCATTGGCCAGTGATCAGTTCTCTACTTCGGCTTTGGTGCGTGGCGCGTATTTCACTTCTGTGTATC
AACAGGGCGTACCCACAAACGCATTTGATGATGCTGCTTCGCGTCGTTATGGGCTAGATCATGCCATTAATAAAGCACAG
CAAGCCAAAAACTCAACGGTTTATTTCACACAAAAACTGTTTAATAACATCATCTATCCTGAAGCTGGGCTTGCCTCAGA
TAATTTTCGTGTTGCGAAGCAAAAACGGCGCTTGATTGGTTTGTCAGTAGTGGCTTGTTCTATTGCAACTGTGTTATTAG
TCGGCACTTGGCATCGTTATTACCTTAGTAACGTGAAGCATTCTGATGCGGTGTTAGCGAAAGTAAACGAGTACAAAAAC
GAGTTCCCCTCTAATCTTTATTTAGCCTCTCAGCAAGATGTTCTCGTGCCGCTCAATAAAATTCGAGAGGCGACATTGGA
GTTTGGCTTCTTCCGTGATAAACCACAGTACATTTCCGATTTTGGTTTGTACCAAGGCCACACGATTGGTCCAATGGTTG
AAGCGACCTATTTGAATTTATTGGAAAACCGCTTTTTACCTCTACTAATGGCTGATGTCGTTGTGCAGCTTAACCAAGCC
AAAACAGATGAAGAGAAACTGGCCGTGCTGCGTGTTTATCGCATGATGGTAGATAAAAGCGGCCGCTATAAGGATTATGT
ACTCGATTATTTCTCCAAATACTGGCAGCAAGAGTTCGCTGGGCAACGCGTGATCCAAGAAGAACTACTGGGGCATTTAG
ACTACGCCATGTTACACACGGATTTAGCGGGTGATCGCGCTCGCGGTGATCGCAATGCAGAGTCGGTGATGAAGCCTTAT
GATCAGGTGGTGGCGAAAGTGCAGTCTGATCTTGGTTCTATGCCTAACGATCAACGTGTTTACCGTAATTTGAAGTTGAA
TGCACAAACGGTTCTTGGCCCCTCGATCAATTTACGCAACTTGATTGGCCCCGTCTTTGATGTGGTTTTTGAAGAGCGAG
TGGTCAACAGCAGCTCGCTGTATATTCCGCAAATGCTCACCAAAAAAGGGTTTGAAGATTACTTCATGCCGCAATCAGAA
TCCGTTTCTGAGCTGGCCTTGATCGACAGTTGGGTACTAGGGCAATCCAAATCGGCTCAATTTAGCGAAGCGGATAAGCA
TGACCTTCGTAACAAAATCCGCAGCTTGTATGTGGCGGACTACACCAATACCTGGCGTGCGGCTCTTAATGAAATCAGCG
TTAAGTATTTTAGCGACATCAATGACGCGGTGTCAGTATTAGAAAACTTTACTGGCAACATTGAACCGATGCAGCGTTTG
CTTCGCACTTTAGAAAGCAATACTCAAATGTATGCCAGTGTGCCTAAAGATGAAGCCGCGCAAAAAGAGCTGTTAAAAAA
TCCAAAATACAAAGTTGCTTCGCAGATTGATGCGCCCTTTGCTGAACTAAACGCTATGCTCAAGCCAGTCGGTGATAAGC
CAGCGTACATCAATGAAGTATTAGTGGCGGTGGAAGAGTTACAAAATTACCTCAAATCTATTCAAGAAGCGCCAGATGTT
GGTATGGCTGCGCTGGATGCCACCAAAGCACGCGTAAAATTAGTGAACGCTGATCCAATTTACACGGTGAAACGTATTGC
ATCAGGTTTGCCTAAACCGCTCGATACAATGGTGTCTAAACTGGCGGATGAGAGTTGGTATGTTGTCAAACAAGAAGCGA
TTAAGCACCTTGAAGTTCGTTGGCATGATGATGTTTACAAAACCTTTGAAGAGAAATTGGCTGGGCGCTATCCATTTAAT
GAGTCTTCCAACAAAGACAGTTCTCTTTCTGATTTTGAAGCATTTTTTGCGCCCAACGGCACATTAGACAACTTCTACAA
CAACCAGCTAAGAATGTTTGTGGAAGAAAATATTGCGGTCAATGATGACGATAACGCGCAGTCGATTATTCGCAAAGACG
TTCTAGAACAGCTCAAGCAGGCGAAGAAAATTCAGCAAGCTTTCTTTAACCGCAAAGGGGTCTTAGACGTTAGTTTCTCG
GTTGAACCGCTACGCTTGAGCAACAACAAACGCCGCAGTGTGCTGAATGTTGACGGTCAATATCTCGCTTATAGTCACGG
CCCAAGAGACAGTGTCGAGTTGATTTGGCCAAATACGCTGCGTGATTCTGCGATCTCTAAAGTAACGTTAGTGCCGACTA
AAAATAATATGTCGCCACGAAGTATTAATATCCAAGGTCCGTGGGCATTTTTCCGCTTGCTTGAACAAGGTGATGTGGTG
GGTGCTAGTTCCACGTCGGTTGACTTTAAGTTTGCAGTTGACGGTGGTGAGATGATTTATCGACTCAATTCAGAGGCAGA
TATTAACCCATTCACCGAGCGGTTATTTAAGTCATTCAAACTCTCGAAAACCCTTTACTAA
ATTGCCGCGGCGATGCCTATTTTGCTATTTAGCACATTCATTCTATTGAATGTTGCGATTTGGTGGGCAGGCCCTTGGCT
AGAGATCGCGGGTAATAAGCCACTTGAATCGATAACCGCTCGCGCAGTGGCAAGTAGCTTGTTCACTTTAGGTGCATTGG
CCGTTTGGGGTGTTTGGCAGTGGCGAAAATTGCAAGGTTTTAAAGCCGAGCAAAAGCGTGAAGATCAGCTTCGCCAAGAT
CCAATTAAAGCCTATGAAGAGCGCCAAGAGGTTGAGCTGAACCAAGTGATGGTCAGCATGAAAGAGAACCTCAATAAACG
TAATTATCTTTATGCGTTGCCATGGTATTTAGTGCTCGGTTTGGAAAATGCCGGTAAAACCAGCCTCATCAACCGCTCTG
GGCAAAACTTTGTTTTTTCTTCGGTAATGCGAGCCTCGGGTCAAAAAAGTGAAAACCCGTATTCGTTTGATTGGTGGATT
GGCGATGAATCGGTATTGATTGATCCAGATGGAGAGCTACTGACACAAGGTAACCATAGCAATGATAACGATGGTGCGAT
GGAGCGCCGTTTGTGGCTTCATTTTGTTAATTGGTTGGAGAGAACGCGTAGCCGTCGTCCACTCAACGGTATTGTTTTAG
CGTTAGACGTTTCTCACTTAGCAACCGCAACTGCAACCGAGCGTAAAGCTTACGCCAATTTATTACGTGCTCGCTTACGT
GAACTGATGGAGACGTTATCCACGCGTCTACCTGTCTATATTGCATTGACGAAATTGGATTTATTACACGGTTTTGAACC
TTTCTTTAAACATTACTCGCGCAGTCAGCGTGAAGATGTGCTGGGCTTTACCTTTTCGCTTGACTCTATTGATGACTTAG
ATAATTGGCTCAACGAGTTTTCAAAAGACTACAGTCAGTTTGTTGAGCGCATTAATGATGTACTGCCGCATGCGGTTTCT
GTACCTATGGATCTCGAAGAGCGCAATGCTATTTATAGTTTCACGCGTCAAATTTCTGGTTTAAAAGAGATCTTATTACA
ATTTTTCAACGATGCATTGGCCAGTGATCAGTTCTCTACTTCGGCTTTGGTGCGTGGCGCGTATTTCACTTCTGTGTATC
AACAGGGCGTACCCACAAACGCATTTGATGATGCTGCTTCGCGTCGTTATGGGCTAGATCATGCCATTAATAAAGCACAG
CAAGCCAAAAACTCAACGGTTTATTTCACACAAAAACTGTTTAATAACATCATCTATCCTGAAGCTGGGCTTGCCTCAGA
TAATTTTCGTGTTGCGAAGCAAAAACGGCGCTTGATTGGTTTGTCAGTAGTGGCTTGTTCTATTGCAACTGTGTTATTAG
TCGGCACTTGGCATCGTTATTACCTTAGTAACGTGAAGCATTCTGATGCGGTGTTAGCGAAAGTAAACGAGTACAAAAAC
GAGTTCCCCTCTAATCTTTATTTAGCCTCTCAGCAAGATGTTCTCGTGCCGCTCAATAAAATTCGAGAGGCGACATTGGA
GTTTGGCTTCTTCCGTGATAAACCACAGTACATTTCCGATTTTGGTTTGTACCAAGGCCACACGATTGGTCCAATGGTTG
AAGCGACCTATTTGAATTTATTGGAAAACCGCTTTTTACCTCTACTAATGGCTGATGTCGTTGTGCAGCTTAACCAAGCC
AAAACAGATGAAGAGAAACTGGCCGTGCTGCGTGTTTATCGCATGATGGTAGATAAAAGCGGCCGCTATAAGGATTATGT
ACTCGATTATTTCTCCAAATACTGGCAGCAAGAGTTCGCTGGGCAACGCGTGATCCAAGAAGAACTACTGGGGCATTTAG
ACTACGCCATGTTACACACGGATTTAGCGGGTGATCGCGCTCGCGGTGATCGCAATGCAGAGTCGGTGATGAAGCCTTAT
GATCAGGTGGTGGCGAAAGTGCAGTCTGATCTTGGTTCTATGCCTAACGATCAACGTGTTTACCGTAATTTGAAGTTGAA
TGCACAAACGGTTCTTGGCCCCTCGATCAATTTACGCAACTTGATTGGCCCCGTCTTTGATGTGGTTTTTGAAGAGCGAG
TGGTCAACAGCAGCTCGCTGTATATTCCGCAAATGCTCACCAAAAAAGGGTTTGAAGATTACTTCATGCCGCAATCAGAA
TCCGTTTCTGAGCTGGCCTTGATCGACAGTTGGGTACTAGGGCAATCCAAATCGGCTCAATTTAGCGAAGCGGATAAGCA
TGACCTTCGTAACAAAATCCGCAGCTTGTATGTGGCGGACTACACCAATACCTGGCGTGCGGCTCTTAATGAAATCAGCG
TTAAGTATTTTAGCGACATCAATGACGCGGTGTCAGTATTAGAAAACTTTACTGGCAACATTGAACCGATGCAGCGTTTG
CTTCGCACTTTAGAAAGCAATACTCAAATGTATGCCAGTGTGCCTAAAGATGAAGCCGCGCAAAAAGAGCTGTTAAAAAA
TCCAAAATACAAAGTTGCTTCGCAGATTGATGCGCCCTTTGCTGAACTAAACGCTATGCTCAAGCCAGTCGGTGATAAGC
CAGCGTACATCAATGAAGTATTAGTGGCGGTGGAAGAGTTACAAAATTACCTCAAATCTATTCAAGAAGCGCCAGATGTT
GGTATGGCTGCGCTGGATGCCACCAAAGCACGCGTAAAATTAGTGAACGCTGATCCAATTTACACGGTGAAACGTATTGC
ATCAGGTTTGCCTAAACCGCTCGATACAATGGTGTCTAAACTGGCGGATGAGAGTTGGTATGTTGTCAAACAAGAAGCGA
TTAAGCACCTTGAAGTTCGTTGGCATGATGATGTTTACAAAACCTTTGAAGAGAAATTGGCTGGGCGCTATCCATTTAAT
GAGTCTTCCAACAAAGACAGTTCTCTTTCTGATTTTGAAGCATTTTTTGCGCCCAACGGCACATTAGACAACTTCTACAA
CAACCAGCTAAGAATGTTTGTGGAAGAAAATATTGCGGTCAATGATGACGATAACGCGCAGTCGATTATTCGCAAAGACG
TTCTAGAACAGCTCAAGCAGGCGAAGAAAATTCAGCAAGCTTTCTTTAACCGCAAAGGGGTCTTAGACGTTAGTTTCTCG
GTTGAACCGCTACGCTTGAGCAACAACAAACGCCGCAGTGTGCTGAATGTTGACGGTCAATATCTCGCTTATAGTCACGG
CCCAAGAGACAGTGTCGAGTTGATTTGGCCAAATACGCTGCGTGATTCTGCGATCTCTAAAGTAACGTTAGTGCCGACTA
AAAATAATATGTCGCCACGAAGTATTAATATCCAAGGTCCGTGGGCATTTTTCCGCTTGCTTGAACAAGGTGATGTGGTG
GGTGCTAGTTCCACGTCGGTTGACTTTAAGTTTGCAGTTGACGGTGGTGAGATGATTTATCGACTCAATTCAGAGGCAGA
TATTAACCCATTCACCGAGCGGTTATTTAAGTCATTCAAACTCTCGAAAACCCTTTACTAA