Detailed information of component protein


Summary

Component ID T6CP013739
Component protein type TssM
T6SS ID (Type) T6SS01063 experimental (Type i3)
Strain Bradyrhizobium japonicum strain J5
Replicon chromosome
Sequence Protein sequence (1181 a.a.); Nucleotide sequence (3543 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BKD09_RS17280
Coordinate (Strand) 3571105..3574647 (+)
NCBI ID WP_071911375.1
RefSeq NZ_CP017637
Uniprot ID A0A1L3F9V3_BRAJP
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 5.2e-87 184..439
IcmF-related PF06761.14 1.3e-73 495..803
IcmF_C PF06744.14 1.4e-30 1037..1161
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-8 MLAKDILR
Transmembrane helix 9-31 IVLYGVGLSSLGAVIYLAGPFIA
Outside 32-40 FGDWRPLEN
Transmembrane helix 41-63 HIVRQIAILLLTTVVAGVVGLNL
Inside 64-430 FKRRKGAKEIADGIAGADQPVSDEPVLKERMKDALATLKTASGNKSGYLYDLPWYVIIGPPGAGKTTALVNSGLKFPLARGATPAAIAGVGGTRYCDWWFTEEAVLIDTAGRYTTQDSNSKADKDSWLAFLDILKKSRSRQPINGVLVAISIEDILTLPKQELALHADAIRMRLLELHQRLKVSFPVYALFTKADLVAGFTEYFAYLGEAGRRQVWGATFQTADKTQNLVGQIPVEFDRLLERLSEETLDRLQDEPTPQHRVQLFGFPAQMARLKPQIHDFLNQIFEPTRYHVNANLRGFYFTSGTQQGTPIDQLIGSLARTFGAEEVGSGSYSGTGKSFFLADLISKVIIGEADWVSTDRAAVRRA
Transmembrane helix 431-453 LILKTAALSLIGLVSIGLIAAWL
Outside 454-1180 MSYKRNSDLIEQSLQADSEYAAAGAPLIKQTLIADHDLDKVLPLLYRLRNAPAGYGSRGESVPLSARYGLSQHARLLSPSKAAYHTALERMFRPRLLYRLEEQLNARINEPAFVYEALKVYLMVGGLQSPDRELIRSWMQRDWADNLYPGATNAEGRRLLDENLVAMFDLETEQQPLVELDGRLIKQAQSSLARLSVSQRAYEFLKSEARASTAGDWIASRHGGPDMGVVFETTTGQPLDAVRVPEFFTYNGFHQKFVARLSGLSERMKRERWVLGDAGQQSAIDQQYDNLAGDLLNIYSNDFVTTWRTALGSLRLKKLLADKPKYEVLRALSAPTSPMRQILESVRDETVLTKERAKPANASAPATAPAPALFTNAQDGPPGATIEAQFKPYHAALEGESTRRPIDSTVANLNDIAQNLTLIVENPQLTAQATAALQTQVAALRNNASRMPPPFSDMLRAAAAEFEGSIAASTAGQILQTLRDQVTPVCQQTVTNRYPFVRSSGQEVPLADFAKLFSPNGVMDKFFTQYLAPYADTSRSDWAWRKESPVGRSFSPDTLKQFQNAAYIRDAFFQTGGGVPAVSFAIRPPGAPGPGVTVKTEIGGTTIASPTIPGPATSSLFGGPQPTPPPPPQPQSNSPTTVLWPGPAPRTAISVSNDTGAPSVLERIGPWSLFRMLEAGSLVVKAETASATFIVAGRELNYQISTGSLRNPLNLSVLREFRCPTGI


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9923 - -


  Protein sequence: 1181 a.a.    

>T6CP013739 NZ_CP017637:3571105-3574647 [Bradyrhizobium japonicum] [TssM]
MLAKDILRIVLYGVGLSSLGAVIYLAGPFIAFGDWRPLENHIVRQIAILLLTTVVAGVVGLNLFKRRKGAKEIADGIAGA
DQPVSDEPVLKERMKDALATLKTASGNKSGYLYDLPWYVIIGPPGAGKTTALVNSGLKFPLARGATPAAIAGVGGTRYCD
WWFTEEAVLIDTAGRYTTQDSNSKADKDSWLAFLDILKKSRSRQPINGVLVAISIEDILTLPKQELALHADAIRMRLLEL
HQRLKVSFPVYALFTKADLVAGFTEYFAYLGEAGRRQVWGATFQTADKTQNLVGQIPVEFDRLLERLSEETLDRLQDEPT
PQHRVQLFGFPAQMARLKPQIHDFLNQIFEPTRYHVNANLRGFYFTSGTQQGTPIDQLIGSLARTFGAEEVGSGSYSGTG
KSFFLADLISKVIIGEADWVSTDRAAVRRALILKTAALSLIGLVSIGLIAAWLMSYKRNSDLIEQSLQADSEYAAAGAPL
IKQTLIADHDLDKVLPLLYRLRNAPAGYGSRGESVPLSARYGLSQHARLLSPSKAAYHTALERMFRPRLLYRLEEQLNAR
INEPAFVYEALKVYLMVGGLQSPDRELIRSWMQRDWADNLYPGATNAEGRRLLDENLVAMFDLETEQQPLVELDGRLIKQ
AQSSLARLSVSQRAYEFLKSEARASTAGDWIASRHGGPDMGVVFETTTGQPLDAVRVPEFFTYNGFHQKFVARLSGLSER
MKRERWVLGDAGQQSAIDQQYDNLAGDLLNIYSNDFVTTWRTALGSLRLKKLLADKPKYEVLRALSAPTSPMRQILESVR
DETVLTKERAKPANASAPATAPAPALFTNAQDGPPGATIEAQFKPYHAALEGESTRRPIDSTVANLNDIAQNLTLIVENP
QLTAQATAALQTQVAALRNNASRMPPPFSDMLRAAAAEFEGSIAASTAGQILQTLRDQVTPVCQQTVTNRYPFVRSSGQE
VPLADFAKLFSPNGVMDKFFTQYLAPYADTSRSDWAWRKESPVGRSFSPDTLKQFQNAAYIRDAFFQTGGGVPAVSFAIR
PPGAPGPGVTVKTEIGGTTIASPTIPGPATSSLFGGPQPTPPPPPQPQSNSPTTVLWPGPAPRTAISVSNDTGAPSVLER
IGPWSLFRMLEAGSLVVKAETASATFIVAGRELNYQISTGSLRNPLNLSVLREFRCPTGI*

  Nucleotide sequence: 3543 bp    

>T6CP013739 NZ_CP017637:3571105-3574647 [Bradyrhizobium japonicum] [TssM]
ATGCTTGCCAAGGACATTCTTCGCATCGTTCTCTACGGAGTGGGGCTCAGCTCGCTCGGAGCGGTGATCTATCTCGCTGG
TCCCTTCATCGCGTTCGGCGACTGGCGACCGCTGGAGAACCATATCGTCCGGCAGATCGCTATTCTGCTGCTCACCACGG
TCGTGGCAGGCGTCGTTGGCCTCAATCTGTTCAAGCGGCGCAAGGGTGCCAAGGAGATTGCGGATGGCATCGCTGGTGCG
GACCAGCCTGTCAGCGATGAACCGGTGCTCAAGGAGCGCATGAAGGATGCGCTCGCGACGCTGAAGACCGCCAGCGGCAA
CAAGTCGGGCTATCTCTACGATCTGCCGTGGTACGTCATCATCGGTCCGCCCGGCGCCGGCAAGACCACGGCGCTGGTCA
ATTCCGGGCTCAAATTTCCGCTCGCGCGCGGGGCGACTCCCGCAGCGATCGCGGGGGTCGGCGGTACGCGCTACTGCGAT
TGGTGGTTTACCGAGGAGGCAGTGCTGATCGATACCGCCGGCCGCTACACGACCCAGGACTCCAACAGCAAGGCCGACAA
GGACAGCTGGCTGGCGTTCCTGGATATCCTGAAGAAAAGCCGGTCGCGCCAGCCGATCAACGGCGTCCTTGTCGCCATCA
GTATCGAAGACATCCTGACGCTGCCGAAGCAGGAACTCGCACTTCACGCCGATGCCATCAGGATGCGCCTGCTCGAGTTG
CATCAGAGGCTCAAGGTCAGCTTCCCGGTCTACGCGCTATTTACCAAAGCCGATCTCGTTGCCGGCTTCACCGAGTATTT
CGCCTATCTCGGCGAGGCCGGCCGCCGCCAGGTCTGGGGCGCCACGTTCCAGACCGCAGACAAGACCCAGAATCTGGTCG
GGCAGATCCCGGTCGAGTTCGACCGTCTGCTGGAGCGCCTGAGCGAGGAGACGCTCGACCGTCTGCAGGACGAGCCGACG
CCCCAGCACCGCGTGCAATTGTTCGGCTTTCCGGCACAGATGGCGCGGCTCAAGCCCCAGATCCACGACTTCCTCAACCA
GATCTTCGAGCCGACGCGCTATCACGTGAATGCGAACCTGCGCGGTTTCTACTTTACCTCGGGTACGCAGCAGGGCACGC
CGATCGACCAATTGATCGGGTCGCTGGCACGTACGTTCGGCGCTGAGGAGGTCGGTTCCGGATCCTATTCGGGCACGGGC
AAGAGCTTCTTCCTCGCCGATCTGATTTCCAAGGTCATCATCGGCGAGGCCGACTGGGTGTCGACCGACCGTGCCGCGGT
GCGCCGCGCGCTGATCCTGAAAACCGCGGCCCTGTCATTGATCGGCCTGGTCTCGATCGGACTGATCGCAGCTTGGCTGA
TGAGCTACAAGCGGAACTCCGATTTGATCGAGCAAAGCCTGCAGGCGGATAGCGAGTATGCGGCTGCCGGCGCACCACTT
ATCAAGCAGACGCTGATTGCCGATCATGATCTCGACAAGGTGCTGCCACTACTCTACCGGCTGCGCAACGCCCCGGCAGG
CTACGGATCGCGCGGCGAGTCGGTGCCTCTGTCGGCTCGCTACGGGCTCAGTCAGCACGCGCGGCTGCTGTCGCCCTCGA
AGGCGGCCTATCATACGGCGCTTGAGCGCATGTTCCGGCCGCGCCTGCTGTATCGCCTCGAGGAGCAGCTCAATGCGCGC
ATCAACGAACCTGCCTTCGTCTACGAGGCGCTCAAGGTCTATCTGATGGTCGGCGGCCTGCAGTCGCCGGACCGCGAGCT
GATCCGCTCCTGGATGCAGCGTGACTGGGCGGACAACCTCTATCCTGGCGCCACCAACGCGGAAGGGCGGCGTCTGCTCG
ACGAGAACCTCGTGGCAATGTTCGACCTCGAGACCGAGCAGCAGCCGCTGGTCGAACTCGACGGCCGGTTGATCAAGCAG
GCCCAGAGCTCCTTGGCGCGCCTGAGCGTGTCGCAACGTGCCTACGAGTTTCTGAAATCGGAGGCTCGCGCATCGACCGC
TGGCGACTGGATCGCTAGCCGCCATGGCGGACCCGACATGGGTGTCGTGTTCGAAACCACGACGGGGCAGCCTTTGGACG
CGGTGCGGGTACCTGAGTTCTTCACGTATAACGGCTTCCATCAGAAGTTCGTCGCCCGGCTGTCCGGATTGTCTGAGCGG
ATGAAGCGGGAGCGATGGGTTTTGGGTGATGCAGGCCAGCAGTCGGCCATCGACCAGCAGTACGACAATCTGGCCGGCGA
TCTGCTCAACATCTATTCCAACGATTTCGTGACGACCTGGCGAACGGCGCTCGGGAGCCTGCGCCTGAAGAAGCTGCTCG
CCGACAAGCCGAAATACGAGGTGCTGCGGGCGCTTTCGGCGCCGACCTCGCCGATGCGGCAAATTCTGGAATCGGTGCGC
GACGAGACGGTGCTGACCAAGGAACGGGCGAAGCCGGCAAATGCTTCGGCGCCAGCTACCGCACCGGCGCCGGCACTCTT
CACGAATGCCCAGGATGGACCGCCTGGCGCGACGATCGAGGCGCAGTTCAAACCTTACCACGCGGCGCTCGAAGGCGAGA
GCACCCGCCGGCCGATCGACTCGACCGTCGCGAACCTGAACGACATCGCGCAGAACCTGACGTTGATCGTCGAGAATCCC
CAACTGACAGCACAGGCCACGGCTGCGCTTCAGACCCAGGTCGCAGCGCTCCGCAACAATGCCTCGCGCATGCCGCCGCC
GTTCTCCGACATGCTGCGCGCAGCAGCCGCGGAGTTCGAAGGCAGCATCGCGGCTTCAACCGCCGGGCAAATCCTGCAAA
CGTTGCGCGATCAGGTCACGCCGGTCTGCCAACAGACCGTGACCAACCGCTACCCGTTTGTCCGCAGCAGCGGCCAGGAA
GTCCCGCTGGCGGATTTTGCCAAATTGTTCAGTCCGAATGGCGTTATGGACAAGTTCTTCACCCAGTACCTGGCACCTTA
TGCCGACACGTCGCGGTCGGACTGGGCTTGGCGCAAGGAGAGCCCCGTTGGTCGGTCGTTCTCGCCCGACACGCTCAAAC
AATTCCAGAATGCGGCTTATATCCGGGATGCGTTCTTCCAGACGGGCGGCGGTGTGCCCGCCGTTTCCTTCGCGATCCGG
CCTCCTGGGGCACCGGGGCCAGGCGTGACCGTTAAGACCGAGATCGGCGGTACCACGATTGCGAGCCCAACCATTCCCGG
ACCGGCCACGTCGTCCTTGTTCGGCGGTCCGCAACCGACGCCACCGCCACCGCCACAGCCACAGAGCAACTCGCCAACGA
CCGTCCTATGGCCGGGCCCAGCGCCGCGAACGGCAATTTCAGTTAGTAACGACACCGGCGCGCCATCCGTGCTTGAGCGC
ATTGGTCCCTGGTCGTTATTTCGGATGCTGGAGGCGGGCTCGTTGGTCGTGAAAGCGGAAACGGCCAGCGCGACTTTCAT
CGTCGCGGGGCGCGAGCTCAACTATCAAATTTCGACTGGGTCGTTGCGCAACCCGCTGAACCTGTCAGTGCTGCGCGAGT
TTCGTTGCCCGACCGGGATATGA