Detailed information of component protein


Summary

Component ID T6CP013738
Component protein type TssL
T6SS ID (Type) T6SS01063 experimental (Type i3)
Strain Bradyrhizobium japonicum strain J5
Replicon chromosome
Sequence Protein sequence (509 a.a.); Nucleotide sequence (1527 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BKD09_RS17275
Coordinate (Strand) 3569535..3571061 (+)
NCBI ID WP_071911373.1
RefSeq NZ_CP017637
Uniprot ID A0A1L3F9T9_BRAJP
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 8.3e-55 118..319
OmpA PF00691.22 1.4e-11 400..498
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-294 MSDRDKPVNPFGRGERTIIRPNPGGKLPPAPTSQPPPGEGPAARPGYSPSPVPSPGAPAPPPSFSPVPQGSNYASAPASHPAEEWISTPAQAQAQQPMLPPGPPLRVDDLVAPNANPVMRAAGPLLQLLGRLRVALMRASFASLMEQVADAIKFFEKDIRSAGISEHQANTAKYILCATADDIVQHIPTEERHVWTQYSMLSRFFGERVGGVRFFEILDHLKVDPLVNYPVLELQHACLALGFQGIHRTSAGGLANLQLIQRNLYELLRRVRPKTMSDLSPNWRGQALAGRRQR
Transmembrane helix 295-317 LRVPVWLVAAVVAALLTGSYFVL
Outside 318-508 RTLLAGRAENAAEVALALHPAGPIELKRQIVAPPPPPPPPPPPDRITQLQRIRNALAKENTACAMTADQTPSFIVIRVCDLALFASGQATILDGFKPIALRVAATLDKEPGRIRVVGHTDSSPIRTVRFPTNFELSVERAKAVAATLKPGLTDGSRVDVEGKGADAPIGSNTTPEGRAKNRRVEIFIERSE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.5819 - -


  Protein sequence: 509 a.a.    

>T6CP013738 NZ_CP017637:3569535-3571061 [Bradyrhizobium japonicum] [TssL]
MSDRDKPVNPFGRGERTIIRPNPGGKLPPAPTSQPPPGEGPAARPGYSPSPVPSPGAPAPPPSFSPVPQGSNYASAPASH
PAEEWISTPAQAQAQQPMLPPGPPLRVDDLVAPNANPVMRAAGPLLQLLGRLRVALMRASFASLMEQVADAIKFFEKDIR
SAGISEHQANTAKYILCATADDIVQHIPTEERHVWTQYSMLSRFFGERVGGVRFFEILDHLKVDPLVNYPVLELQHACLA
LGFQGIHRTSAGGLANLQLIQRNLYELLRRVRPKTMSDLSPNWRGQALAGRRQRLRVPVWLVAAVVAALLTGSYFVLRTL
LAGRAENAAEVALALHPAGPIELKRQIVAPPPPPPPPPPPDRITQLQRIRNALAKENTACAMTADQTPSFIVIRVCDLAL
FASGQATILDGFKPIALRVAATLDKEPGRIRVVGHTDSSPIRTVRFPTNFELSVERAKAVAATLKPGLTDGSRVDVEGKG
ADAPIGSNTTPEGRAKNRRVEIFIERSE*

  Nucleotide sequence: 1527 bp    

>T6CP013738 NZ_CP017637:3569535-3571061 [Bradyrhizobium japonicum] [TssL]
ATGAGCGACAGGGACAAGCCGGTCAATCCTTTCGGTCGGGGCGAACGGACGATCATCCGTCCCAACCCCGGCGGGAAGTT
GCCGCCGGCGCCGACCTCTCAGCCTCCGCCGGGCGAGGGGCCTGCGGCACGTCCAGGCTATTCGCCATCACCGGTACCCT
CTCCCGGCGCGCCGGCGCCACCGCCATCATTTTCTCCTGTCCCGCAGGGCTCGAACTACGCCTCCGCGCCGGCCAGCCAT
CCCGCTGAGGAATGGATATCGACACCCGCGCAAGCCCAGGCGCAGCAGCCCATGCTACCGCCAGGACCGCCGTTGCGCGT
CGACGATCTCGTCGCGCCAAATGCCAATCCAGTGATGCGCGCTGCGGGCCCGTTGCTGCAGTTGCTTGGTCGGTTGCGGG
TGGCGCTCATGCGCGCGTCATTCGCGAGTTTGATGGAGCAGGTTGCAGACGCGATCAAGTTTTTCGAGAAGGACATCCGT
TCCGCGGGCATATCCGAGCACCAGGCCAACACGGCGAAATACATCCTGTGTGCCACTGCCGACGATATCGTGCAGCACAT
CCCGACCGAGGAGCGCCACGTCTGGACGCAATATTCGATGCTGAGCCGGTTCTTCGGCGAGCGTGTCGGCGGCGTCCGTT
TCTTCGAGATTCTCGACCATTTGAAGGTTGATCCACTGGTCAACTATCCGGTCCTCGAACTTCAGCACGCCTGCCTTGCG
CTTGGTTTTCAGGGCATTCACCGGACGTCAGCGGGCGGACTTGCGAACCTGCAACTGATCCAGCGTAACCTCTATGAATT
GCTGCGCCGGGTTCGGCCGAAGACGATGAGCGACCTGTCGCCCAACTGGCGCGGGCAGGCGCTGGCGGGGCGCCGGCAGC
GTCTGCGCGTTCCGGTCTGGCTCGTGGCGGCCGTCGTCGCCGCGCTGCTGACCGGGTCCTATTTCGTCCTGCGGACGCTC
CTCGCTGGCCGGGCAGAGAATGCTGCCGAGGTGGCGCTCGCGCTCCATCCAGCCGGCCCGATCGAGCTCAAGCGCCAGAT
CGTTGCACCACCGCCGCCGCCTCCACCTCCGCCGCCACCGGATCGCATCACCCAGTTGCAACGCATCCGCAACGCGCTCG
CGAAGGAGAACACCGCCTGTGCCATGACGGCGGACCAGACCCCGAGCTTCATCGTGATCAGGGTCTGCGATCTGGCGTTG
TTCGCGTCGGGCCAGGCGACCATCCTCGACGGTTTCAAGCCAATCGCACTCCGCGTCGCAGCAACCCTCGACAAGGAGCC
GGGGCGGATCCGGGTCGTCGGCCATACCGACAGTTCGCCGATCCGCACAGTCCGCTTCCCGACAAATTTCGAGTTGTCGG
TCGAGCGCGCCAAGGCGGTTGCCGCGACACTGAAGCCTGGCTTGACCGATGGTTCGCGCGTCGACGTCGAGGGCAAAGGA
GCAGATGCGCCAATCGGTAGCAACACGACACCCGAGGGACGCGCCAAGAACCGGCGCGTCGAGATCTTTATCGAGCGCAG
CGAATAG