Detailed information of component protein


Summary

Component ID T6CP013736
Component protein type TssI
T6SS ID (Type) T6SS01063 experimental (Type i3)
Strain Bradyrhizobium japonicum strain J5
Replicon chromosome
Sequence Protein sequence (623 a.a.); Nucleotide sequence (1869 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BKD09_RS17255
Coordinate (Strand) 3564344..3566212 (+)
NCBI ID WP_071911367.1
RefSeq NZ_CP017637
Uniprot ID A0A1L3F9T7_BRAJP
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 1.2e-91 31..330
Phage_base_V PF04717.14 3.8e-10 392..457
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-622 MGQLTQDTRLCELKTPLGKDVLVFAGFEGSEGLSELFEYRIECLSEDADLDFDRAIGQQCTLTIKLHEKEREFSGILTEAQWLGVKNDYFSYRIVLRPWLWLLSRTTDCRIFQDKKAPDIIKEVFNERGFTDYESKLTEEGSCPKLEYCVQYRETDMNFVCRLMEQHGIYYFFKHEGGKHTLVLADSKSSHSPIKGLATIPYIPLAGADRRGEQHIYEWVSERRFRTGKIELNDYNYQKPNAQMISDAKGSEHYTRSEMEFYDYPGKFKEKSDGERYAKIQLQAEQAMDRRRRGNGDAVNLFPGGLTKLEKHSKDSQNVEYLVVRAVHSFSIESYRTGGPSDAGQHVYFGSYEFLPSDRPFRALMMTPKPRINGIQTAKVVTKDDNSSEEIDVESLGEIYVRFFWDRKKKRSCRLRVAQVWSGKRWGGQIIPRVGQEVVIEFLEGDPDRPLVIGTVYNDEYKLPYDLPSKKTIAGLKSDSTKGGGGFNEWNFEDKKGSEKITVHAEKDHDVTVRDTETRTIGEAAFGGDTRNTTLKNGNDKLDIQLGGQHITLAMDQSVNAGISITLMANAKITLQVGPSQIILTPAGIVINAPTIAMTAQAGMALAAGGPLVAHGTPTLVG


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9976 - -


  Protein sequence: 623 a.a.    

>T6CP013736 NZ_CP017637:3564344-3566212 [Bradyrhizobium japonicum] [TssI]
MGQLTQDTRLCELKTPLGKDVLVFAGFEGSEGLSELFEYRIECLSEDADLDFDRAIGQQCTLTIKLHEKEREFSGILTEA
QWLGVKNDYFSYRIVLRPWLWLLSRTTDCRIFQDKKAPDIIKEVFNERGFTDYESKLTEEGSCPKLEYCVQYRETDMNFV
CRLMEQHGIYYFFKHEGGKHTLVLADSKSSHSPIKGLATIPYIPLAGADRRGEQHIYEWVSERRFRTGKIELNDYNYQKP
NAQMISDAKGSEHYTRSEMEFYDYPGKFKEKSDGERYAKIQLQAEQAMDRRRRGNGDAVNLFPGGLTKLEKHSKDSQNVE
YLVVRAVHSFSIESYRTGGPSDAGQHVYFGSYEFLPSDRPFRALMMTPKPRINGIQTAKVVTKDDNSSEEIDVESLGEIY
VRFFWDRKKKRSCRLRVAQVWSGKRWGGQIIPRVGQEVVIEFLEGDPDRPLVIGTVYNDEYKLPYDLPSKKTIAGLKSDS
TKGGGGFNEWNFEDKKGSEKITVHAEKDHDVTVRDTETRTIGEAAFGGDTRNTTLKNGNDKLDIQLGGQHITLAMDQSVN
AGISITLMANAKITLQVGPSQIILTPAGIVINAPTIAMTAQAGMALAAGGPLVAHGTPTLVG*

  Nucleotide sequence: 1869 bp    

>T6CP013736 NZ_CP017637:3564344-3566212 [Bradyrhizobium japonicum] [TssI]
ATGGGCCAGCTTACTCAGGATACCCGCCTCTGCGAACTCAAGACGCCTCTTGGCAAGGACGTTTTGGTTTTTGCCGGATT
CGAAGGCTCGGAAGGTCTCAGCGAACTGTTCGAGTATCGCATCGAGTGTCTCAGTGAGGATGCCGACCTCGACTTCGACC
GCGCTATCGGTCAGCAGTGCACGCTGACCATAAAGCTGCATGAAAAGGAGCGCGAGTTCAGCGGCATTCTGACGGAGGCC
CAGTGGCTCGGGGTGAAGAACGACTATTTCAGCTACCGGATCGTGCTGAGGCCCTGGTTATGGCTGTTGTCGCGGACCAC
CGATTGCCGGATCTTTCAGGACAAGAAGGCGCCCGACATCATCAAAGAGGTCTTCAACGAACGCGGCTTCACCGACTATG
AATCCAAACTGACCGAAGAGGGTTCTTGTCCGAAGCTCGAATATTGCGTTCAGTACCGCGAGACCGACATGAACTTCGTG
TGCCGGCTGATGGAGCAGCACGGCATCTACTACTTCTTCAAGCATGAGGGCGGCAAGCACACGCTGGTGTTGGCGGACTC
CAAATCGTCGCACAGTCCGATCAAAGGTCTTGCTACGATTCCCTACATCCCGCTGGCGGGTGCCGACCGGCGAGGCGAGC
AGCACATCTATGAATGGGTATCGGAGCGACGGTTTCGTACCGGCAAGATCGAACTCAACGACTACAACTATCAGAAGCCC
AACGCTCAGATGATCAGCGACGCCAAGGGCTCCGAGCATTACACTCGGTCGGAGATGGAGTTTTACGACTATCCCGGGAA
GTTCAAGGAGAAATCCGACGGCGAGCGCTACGCGAAGATCCAGCTCCAGGCAGAGCAGGCGATGGATCGGCGTCGCCGCG
GCAATGGCGACGCCGTCAACCTGTTCCCCGGTGGGCTGACGAAGCTCGAAAAGCACTCCAAGGACTCGCAGAACGTCGAA
TACCTCGTGGTGCGAGCTGTGCACTCGTTCTCGATCGAATCCTATCGCACCGGCGGTCCGAGCGACGCAGGGCAACACGT
CTATTTCGGCAGCTACGAGTTCTTGCCGAGTGATCGGCCGTTTCGAGCGCTGATGATGACGCCGAAGCCAAGAATCAACG
GCATCCAGACGGCCAAGGTGGTGACCAAAGACGATAATTCGAGCGAGGAAATCGACGTCGAGTCCCTGGGCGAAATCTAT
GTCCGCTTTTTCTGGGATCGGAAGAAGAAGCGGTCGTGCCGGTTGCGCGTGGCTCAGGTTTGGTCTGGCAAGCGCTGGGG
CGGTCAGATCATCCCGCGCGTCGGACAGGAAGTCGTTATCGAATTCCTGGAGGGTGATCCGGACCGGCCCCTGGTGATCG
GCACCGTGTACAACGACGAATACAAGCTGCCCTACGATCTGCCGTCGAAGAAGACGATTGCGGGATTGAAGTCGGACTCC
ACCAAAGGCGGCGGCGGCTTCAACGAGTGGAACTTCGAGGACAAGAAGGGATCCGAGAAGATCACGGTGCATGCCGAGAA
GGACCACGACGTAACGGTCCGCGACACCGAGACACGTACCATCGGCGAAGCTGCATTTGGCGGCGACACGCGCAACACCA
CGCTCAAGAACGGCAATGATAAGCTCGATATCCAGTTGGGTGGTCAGCACATCACTTTGGCCATGGACCAGTCCGTCAAT
GCAGGCATCAGCATCACGCTCATGGCCAATGCCAAGATCACCCTGCAGGTGGGTCCTAGTCAGATCATTCTGACCCCGGC
GGGCATCGTCATCAACGCGCCGACGATCGCCATGACGGCGCAGGCCGGCATGGCGCTTGCTGCCGGTGGTCCGCTGGTCG
CCCACGGTACTCCAACCCTTGTTGGTTGA