Detailed information of component protein
Summary
| Component ID | T6CP013735 |
| Component protein type | TssB |
| T6SS ID (Type) | T6SS01063  (Type i3) |
| Strain | Bradyrhizobium japonicum strain J5 |
| Replicon | chromosome |
| Sequence | Protein sequence (176 a.a.); Nucleotide sequence (528 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | BKD09_RS17245 |
| Coordinate (Strand) | 3562023..3562550 (-) |
| NCBI ID | WP_028156839.1 |
| RefSeq | NZ_CP017637 |
| Uniprot ID | A0A0A3XQL2_BRAJP |
| KEGG ID | bjp:RN69_16305 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS_VipA | PF05591.14 | 4e-51 | 11..163 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-175 | MATDSGQKFIRRNRAPRVHITYEDPYDAERLIELPFVMGVLSDLSGNNPGVEKTKVEERKFLEFDMDNFDSRMAAIQPGVTARVANRLSDGSDEKLSVNLRFNKMADFTPVAVARQVPATAKLLEAREQLANLLRYMDGKVAAEDQLKKLLADPQLMAALRERAISQSTEPGTES |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9965 | - | - |
Protein sequence: 176 a.a.
>T6CP013735 NZ_CP017637:c3562550-3562023 [Bradyrhizobium japonicum] [TssB]
MATDSGQKFIRRNRAPRVHITYEDPYDAERLIELPFVMGVLSDLSGNNPGVEKTKVEERKFLEFDMDNFDSRMAAIQPGV
TARVANRLSDGSDEKLSVNLRFNKMADFTPVAVARQVPATAKLLEAREQLANLLRYMDGKVAAEDQLKKLLADPQLMAAL
RERAISQSTEPGTES*
MATDSGQKFIRRNRAPRVHITYEDPYDAERLIELPFVMGVLSDLSGNNPGVEKTKVEERKFLEFDMDNFDSRMAAIQPGV
TARVANRLSDGSDEKLSVNLRFNKMADFTPVAVARQVPATAKLLEAREQLANLLRYMDGKVAAEDQLKKLLADPQLMAAL
RERAISQSTEPGTES*
Nucleotide sequence: 528 bp
>T6CP013735 NZ_CP017637:c3562550-3562023 [Bradyrhizobium japonicum] [TssB]
ATGGCTACAGATAGCGGTCAAAAATTCATTCGTCGAAATCGCGCACCACGCGTGCACATCACATACGAAGATCCATACGA
CGCCGAGCGGCTGATCGAACTGCCGTTCGTCATGGGCGTCCTCTCGGACCTTTCCGGCAATAACCCCGGCGTCGAGAAGA
CGAAGGTCGAGGAGCGCAAATTCCTCGAGTTCGACATGGACAATTTCGACAGCCGCATGGCGGCAATCCAGCCCGGCGTG
ACGGCGCGCGTCGCCAATCGTCTCAGCGACGGATCAGACGAAAAACTTTCGGTCAATCTGCGCTTCAACAAGATGGCCGA
CTTCACTCCCGTGGCCGTCGCCCGCCAGGTGCCGGCTACAGCCAAGCTGCTCGAGGCGCGCGAACAACTTGCCAACCTGC
TGCGCTACATGGATGGCAAGGTAGCCGCCGAGGACCAGCTGAAGAAACTTCTGGCCGATCCTCAACTGATGGCAGCGCTT
CGGGAACGCGCGATCAGCCAGTCGACCGAACCTGGCACCGAGAGCTAA
ATGGCTACAGATAGCGGTCAAAAATTCATTCGTCGAAATCGCGCACCACGCGTGCACATCACATACGAAGATCCATACGA
CGCCGAGCGGCTGATCGAACTGCCGTTCGTCATGGGCGTCCTCTCGGACCTTTCCGGCAATAACCCCGGCGTCGAGAAGA
CGAAGGTCGAGGAGCGCAAATTCCTCGAGTTCGACATGGACAATTTCGACAGCCGCATGGCGGCAATCCAGCCCGGCGTG
ACGGCGCGCGTCGCCAATCGTCTCAGCGACGGATCAGACGAAAAACTTTCGGTCAATCTGCGCTTCAACAAGATGGCCGA
CTTCACTCCCGTGGCCGTCGCCCGCCAGGTGCCGGCTACAGCCAAGCTGCTCGAGGCGCGCGAACAACTTGCCAACCTGC
TGCGCTACATGGATGGCAAGGTAGCCGCCGAGGACCAGCTGAAGAAACTTCTGGCCGATCCTCAACTGATGGCAGCGCTT
CGGGAACGCGCGATCAGCCAGTCGACCGAACCTGGCACCGAGAGCTAA