Detailed information of component protein
Summary
| Component ID | T6CP013733 |
| Component protein type | TssD |
| T6SS ID (Type) | T6SS01063  (Type i3) |
| Strain | Bradyrhizobium japonicum strain J5 |
| Replicon | chromosome |
| Sequence | Protein sequence (162 a.a.); Nucleotide sequence (486 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | BKD09_RS17235 |
| Coordinate (Strand) | 3559970..3560455 (-) |
| NCBI ID | WP_014493489.1 |
| RefSeq | NZ_CP017637 |
| Uniprot ID | A0A7Z0Q4L7_9BRAD, A0A4Y9V1Y2_9BRAD, A0A1L3FA01_BRAJP, A0A023XLZ1_BRAJP |
| KEGG ID | bjp:RN69_16295 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS_HCP | PF05638.14 | 7.7e-40 | 6..135 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-161 | MAVDIFLKLDPIKGESLDDKHKDEIDILSWSFGESQTGTFHSGSGGGAGKVSVQDLHFTHFVDKATPELFKVCANGKHIDSAILTVRKAGENPLEYLKIEMKQVLVTSVSTGGSHGEDRLTENVSLNFAKVKMIYKTQTEKGGAGASPTFGWDVPANKEWA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9788 | - | - |
Protein sequence: 162 a.a.
>T6CP013733 NZ_CP017637:c3560455-3559970 [Bradyrhizobium japonicum] [TssD]
MAVDIFLKLDPIKGESLDDKHKDEIDILSWSFGESQTGTFHSGSGGGAGKVSVQDLHFTHFVDKATPELFKVCANGKHID
SAILTVRKAGENPLEYLKIEMKQVLVTSVSTGGSHGEDRLTENVSLNFAKVKMIYKTQTEKGGAGASPTFGWDVPANKEW
A*
MAVDIFLKLDPIKGESLDDKHKDEIDILSWSFGESQTGTFHSGSGGGAGKVSVQDLHFTHFVDKATPELFKVCANGKHID
SAILTVRKAGENPLEYLKIEMKQVLVTSVSTGGSHGEDRLTENVSLNFAKVKMIYKTQTEKGGAGASPTFGWDVPANKEW
A*
Nucleotide sequence: 486 bp
>T6CP013733 NZ_CP017637:c3560455-3559970 [Bradyrhizobium japonicum] [TssD]
ATGGCTGTCGACATCTTCCTGAAACTCGATCCGATCAAGGGCGAGTCGCTGGACGACAAGCACAAAGACGAAATCGATAT
TTTGAGCTGGTCGTTCGGCGAGAGCCAGACCGGTACCTTCCACAGCGGATCGGGCGGCGGCGCCGGCAAGGTCAGCGTGC
AGGATCTTCACTTCACGCACTTCGTTGACAAGGCCACGCCGGAACTTTTCAAGGTCTGCGCAAACGGCAAGCATATCGAC
AGCGCGATCCTGACGGTCCGCAAGGCCGGCGAAAACCCGCTGGAATATCTCAAGATCGAGATGAAGCAGGTTCTCGTGAC
GAGCGTATCGACAGGCGGATCGCACGGCGAAGATCGCCTTACCGAAAACGTGAGTCTGAACTTCGCGAAGGTCAAGATGA
TCTACAAGACCCAGACGGAGAAGGGTGGCGCCGGAGCTTCGCCGACGTTTGGCTGGGATGTTCCTGCCAACAAGGAATGG
GCCTGA
ATGGCTGTCGACATCTTCCTGAAACTCGATCCGATCAAGGGCGAGTCGCTGGACGACAAGCACAAAGACGAAATCGATAT
TTTGAGCTGGTCGTTCGGCGAGAGCCAGACCGGTACCTTCCACAGCGGATCGGGCGGCGGCGCCGGCAAGGTCAGCGTGC
AGGATCTTCACTTCACGCACTTCGTTGACAAGGCCACGCCGGAACTTTTCAAGGTCTGCGCAAACGGCAAGCATATCGAC
AGCGCGATCCTGACGGTCCGCAAGGCCGGCGAAAACCCGCTGGAATATCTCAAGATCGAGATGAAGCAGGTTCTCGTGAC
GAGCGTATCGACAGGCGGATCGCACGGCGAAGATCGCCTTACCGAAAACGTGAGTCTGAACTTCGCGAAGGTCAAGATGA
TCTACAAGACCCAGACGGAGAAGGGTGGCGCCGGAGCTTCGCCGACGTTTGGCTGGGATGTTCCTGCCAACAAGGAATGG
GCCTGA