Detailed information of component protein
Summary
| Component ID | T6CP013732 |
| Component protein type | TssE |
| T6SS ID (Type) | T6SS01063  (Type i3) |
| Strain | Bradyrhizobium japonicum strain J5 |
| Replicon | chromosome |
| Sequence | Protein sequence (183 a.a.); Nucleotide sequence (549 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | BKD09_RS17225 |
| Coordinate (Strand) | 3559240..3559788 (-) |
| NCBI ID | WP_155795011.1 |
| RefSeq | NZ_CP017637 |
| Uniprot ID | UPI0012DB5EEB |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| GPW_gp25 | PF04965.16 | 2.1e-17 | 58..159 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-182 | MTIAPKKDRLSPPLMLAFRSAHEARDARKKLNLRDESGERVIAGRRSAGRFPISETLLRREVSHDLETLLNTIALESTLDLNGHDCVRTSILNFGFPDIAHRSIDEVADDELTDALRETLTTYEPRLDRNTIRVRRDGSVGPEQLKLRFIVHADLKAEPLNVPVEFVADVDLDSGDIQINRL |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9816 | - | - |
Protein sequence: 183 a.a.
>T6CP013732 NZ_CP017637:c3559788-3559240 [Bradyrhizobium japonicum] [TssE]
MTIAPKKDRLSPPLMLAFRSAHEARDARKKLNLRDESGERVIAGRRSAGRFPISETLLRREVSHDLETLLNTIALESTLD
LNGHDCVRTSILNFGFPDIAHRSIDEVADDELTDALRETLTTYEPRLDRNTIRVRRDGSVGPEQLKLRFIVHADLKAEPL
NVPVEFVADVDLDSGDIQINRL*
MTIAPKKDRLSPPLMLAFRSAHEARDARKKLNLRDESGERVIAGRRSAGRFPISETLLRREVSHDLETLLNTIALESTLD
LNGHDCVRTSILNFGFPDIAHRSIDEVADDELTDALRETLTTYEPRLDRNTIRVRRDGSVGPEQLKLRFIVHADLKAEPL
NVPVEFVADVDLDSGDIQINRL*
Nucleotide sequence: 549 bp
>T6CP013732 NZ_CP017637:c3559788-3559240 [Bradyrhizobium japonicum] [TssE]
ATGACCATTGCACCAAAGAAGGATCGGCTCTCTCCCCCACTGATGCTGGCATTTCGCTCAGCCCATGAAGCGCGCGACGC
ACGCAAGAAGCTCAATCTACGCGATGAGTCTGGCGAGCGGGTCATTGCAGGGCGGCGGAGCGCGGGCCGATTTCCAATCT
CGGAAACGTTGCTCCGGCGCGAGGTCTCCCATGACCTCGAAACGCTCCTCAACACCATTGCCCTGGAATCGACCCTGGAT
CTGAACGGCCACGATTGCGTCCGAACCTCAATCCTGAACTTTGGCTTCCCTGATATTGCCCACCGTTCGATCGACGAGGT
CGCCGACGACGAGCTCACCGACGCGTTGCGCGAGACGCTCACGACTTATGAACCGAGGCTCGATCGCAACACCATCCGGG
TCCGACGCGACGGCTCGGTCGGCCCCGAGCAACTCAAGCTGCGCTTCATCGTTCACGCCGACCTGAAGGCCGAGCCGCTC
AACGTCCCCGTGGAGTTCGTCGCCGACGTCGACCTCGACAGCGGCGATATCCAGATCAACCGACTTTAG
ATGACCATTGCACCAAAGAAGGATCGGCTCTCTCCCCCACTGATGCTGGCATTTCGCTCAGCCCATGAAGCGCGCGACGC
ACGCAAGAAGCTCAATCTACGCGATGAGTCTGGCGAGCGGGTCATTGCAGGGCGGCGGAGCGCGGGCCGATTTCCAATCT
CGGAAACGTTGCTCCGGCGCGAGGTCTCCCATGACCTCGAAACGCTCCTCAACACCATTGCCCTGGAATCGACCCTGGAT
CTGAACGGCCACGATTGCGTCCGAACCTCAATCCTGAACTTTGGCTTCCCTGATATTGCCCACCGTTCGATCGACGAGGT
CGCCGACGACGAGCTCACCGACGCGTTGCGCGAGACGCTCACGACTTATGAACCGAGGCTCGATCGCAACACCATCCGGG
TCCGACGCGACGGCTCGGTCGGCCCCGAGCAACTCAAGCTGCGCTTCATCGTTCACGCCGACCTGAAGGCCGAGCCGCTC
AACGTCCCCGTGGAGTTCGTCGCCGACGTCGACCTCGACAGCGGCGATATCCAGATCAACCGACTTTAG