Detailed information of component protein


Summary

Component ID T6CP013731
Component protein type TssF
T6SS ID (Type) T6SS01063 experimental (Type i3)
Strain Bradyrhizobium japonicum strain J5
Replicon chromosome
Sequence Protein sequence (656 a.a.); Nucleotide sequence (1968 bp)
Reference
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) BKD09_RS17220
Coordinate (Strand) 3557271..3559238 (-)
NCBI ID WP_071911363.1
RefSeq NZ_CP017637
Uniprot ID A0A1L3F9T5_BRAJP
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_TssF PF05947.14 6e-186 1..652

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-655 MNREFLDFYNRELSLLYEQAGDFAEEYPGIAERLGGLIRDRSDPMITGLLEGAAFLAARVQLKLKHEFPEFTANLLEQLVPNYLAPTPSAMLVTARPPYADPALRDGKKIVRGAYFDATYRERERSLSCRFRLCRDIALWPFDVTGAEYFSTAGALQALGIPVGGDVIAGLRLSLTHRTAARLDEELPDAEARAKPETWFAGCRTSELPIYLSGAESDAIALYEQLISNCRGVYFRHLDDFGDPVVTRAPHDCVQQIGFDENESLFPNDNRVFRGSELLREYFMFPRKFLGFNLTGLRSVMPRLRSKSIDIVFAFDEHNSRLAAAVRPDTFRLYSAPAINLFEKTSDRIPVKSNTHEYHVVPDRSRYLEYEPHQVLEVYAHFPADKDKRPVRPLYSAAVDRTSGSVEGLYFTVRRLPRRRTVGEKTYGASSDYTGTDMFLSLLEPGELSGEGAVAELSVRALCSNRHLTEHLPVGQGGADFRLLDDTSLDLMCVAGPTRPREPIVAQLRSRSEIANSGIVSWRLINMLSLNYLGLVERGGGKNAGALREMLSLFADQFDDATERKIRGVRSIESRPVVRRVRERTGSGAARGLEITITLDEKAFEGSGVFLLGAVLERFFADYSGFNTFTQTVVSTPERGEIMRWPPRMGTRRPL



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9800 - -



  Protein sequence: 656 a.a.    

>T6CP013731 NZ_CP017637:c3559238-3557271 [Bradyrhizobium japonicum] [TssF]
MNREFLDFYNRELSLLYEQAGDFAEEYPGIAERLGGLIRDRSDPMITGLLEGAAFLAARVQLKLKHEFPEFTANLLEQLV
PNYLAPTPSAMLVTARPPYADPALRDGKKIVRGAYFDATYRERERSLSCRFRLCRDIALWPFDVTGAEYFSTAGALQALG
IPVGGDVIAGLRLSLTHRTAARLDEELPDAEARAKPETWFAGCRTSELPIYLSGAESDAIALYEQLISNCRGVYFRHLDD
FGDPVVTRAPHDCVQQIGFDENESLFPNDNRVFRGSELLREYFMFPRKFLGFNLTGLRSVMPRLRSKSIDIVFAFDEHNS
RLAAAVRPDTFRLYSAPAINLFEKTSDRIPVKSNTHEYHVVPDRSRYLEYEPHQVLEVYAHFPADKDKRPVRPLYSAAVD
RTSGSVEGLYFTVRRLPRRRTVGEKTYGASSDYTGTDMFLSLLEPGELSGEGAVAELSVRALCSNRHLTEHLPVGQGGAD
FRLLDDTSLDLMCVAGPTRPREPIVAQLRSRSEIANSGIVSWRLINMLSLNYLGLVERGGGKNAGALREMLSLFADQFDD
ATERKIRGVRSIESRPVVRRVRERTGSGAARGLEITITLDEKAFEGSGVFLLGAVLERFFADYSGFNTFTQTVVSTPERG
EIMRWPPRMGTRRPL*

  Nucleotide sequence: 1968 bp    

>T6CP013731 NZ_CP017637:c3559238-3557271 [Bradyrhizobium japonicum] [TssF]
ATGAACCGCGAATTCCTTGATTTTTACAATCGTGAACTGTCACTGCTCTACGAGCAGGCCGGGGATTTCGCCGAAGAGTA
TCCCGGCATCGCAGAACGCCTCGGTGGGCTGATCCGGGATCGCTCAGATCCCATGATCACGGGCTTGCTGGAAGGTGCCG
CATTTCTCGCCGCTCGCGTTCAGCTCAAGCTCAAGCACGAGTTCCCGGAATTCACTGCAAACCTGCTCGAGCAACTGGTT
CCGAACTACCTTGCCCCGACTCCGTCGGCCATGCTGGTCACGGCACGCCCCCCCTATGCCGACCCAGCGCTACGCGATGG
CAAGAAGATCGTGCGCGGCGCCTATTTCGATGCCACCTATCGCGAGAGAGAACGAAGTCTTTCCTGCCGATTTCGACTTT
GCCGCGATATCGCTCTTTGGCCATTTGACGTCACTGGCGCCGAATACTTCTCGACGGCAGGCGCGCTGCAGGCGCTCGGG
ATTCCCGTCGGCGGGGACGTCATCGCCGGGCTTAGGCTGTCGTTGACCCACCGTACCGCGGCCCGGCTCGATGAGGAGCT
GCCGGACGCAGAGGCCAGGGCCAAGCCGGAAACCTGGTTTGCAGGCTGCCGCACGAGCGAACTTCCGATCTACCTTTCAG
GCGCGGAATCCGACGCCATTGCGCTTTACGAGCAATTGATCTCGAACTGCAGGGGCGTCTACTTCCGGCATCTCGATGAT
TTCGGCGATCCGGTGGTGACCCGTGCGCCCCACGACTGCGTGCAGCAGATTGGTTTCGACGAGAACGAGTCGCTGTTTCC
CAACGACAACCGGGTCTTTCGCGGCTCGGAGCTGCTGCGGGAATATTTCATGTTTCCACGCAAGTTCCTCGGCTTCAACC
TGACGGGACTTCGCAGCGTGATGCCACGGCTGCGCAGCAAATCGATCGATATCGTCTTCGCTTTCGATGAACACAATTCA
CGGCTTGCCGCCGCGGTGCGTCCCGATACCTTCAGGCTCTATAGCGCGCCGGCGATCAACCTGTTCGAGAAGACGAGCGA
TCGCATCCCCGTCAAGTCGAATACGCACGAGTACCATGTGGTGCCCGATCGCAGCCGCTATCTGGAATACGAACCGCACC
AGGTGCTCGAAGTCTACGCGCACTTTCCGGCTGACAAGGACAAGCGGCCGGTGCGCCCGCTGTATTCGGCCGCCGTCGAC
CGGACCAGCGGATCGGTCGAGGGGCTTTATTTCACCGTTCGACGGTTGCCCCGGCGGCGTACAGTGGGAGAAAAGACGTA
TGGCGCATCCTCGGACTACACCGGGACGGACATGTTCCTGTCTCTGCTGGAGCCCGGTGAACTTAGCGGCGAAGGTGCGG
TCGCCGAACTCAGCGTCCGGGCGCTGTGCTCCAACCGGCATCTCACGGAACACTTGCCGGTCGGCCAGGGCGGCGCCGAC
TTCCGCCTGCTGGACGATACTTCCCTTGATCTGATGTGCGTCGCCGGTCCCACCCGACCACGCGAACCGATCGTGGCCCA
GTTGCGCAGCCGCAGTGAGATCGCGAACAGCGGAATCGTCAGCTGGCGGCTGATCAACATGCTGAGCCTGAACTATCTCG
GCCTCGTCGAGCGCGGTGGCGGAAAGAACGCCGGAGCCCTGCGCGAAATGCTGTCCTTGTTCGCCGACCAGTTCGACGAC
GCCACGGAACGCAAGATCCGTGGCGTGCGCAGCATAGAAAGCCGGCCGGTCGTACGGCGGGTGCGCGAGCGCACCGGCAG
CGGCGCAGCGCGCGGCCTGGAGATTACCATCACGCTCGACGAGAAGGCATTCGAGGGCAGCGGCGTGTTCTTGCTCGGAG
CCGTGCTGGAGCGCTTCTTCGCCGACTATTCGGGCTTCAATACCTTCACACAGACGGTCGTCTCGACGCCGGAGCGCGGC
GAGATCATGCGTTGGCCGCCGCGCATGGGCACGCGGAGACCTCTATGA