Detailed information of component protein


Summary

Component ID T6CP013717
Component protein type TssM
T6SS ID (Type) T6SS01061 experimental (Type i1)
Strain Vibrio cholerae 2740-80
Replicon chromosome
Sequence Protein sequence (1167 a.a.); Nucleotide sequence (3501 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BBB50_RS14485
Coordinate (Strand) 131995..135495 (+)
NCBI ID WP_000225015.1
RefSeq NZ_CP016325
Uniprot ID UPI00005F3122
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 3.2e-77 188..445
IcmF-related PF06761.14 3.5e-71 495..809
IcmF_C PF06744.14 4.8e-24 1039..1145
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-4 VVAA
Transmembrane helix 5-27 LPILLFTTFILLNVAIWWAGPWL
Outside 28-36 EVAGYKPLE
Transmembrane helix 37-59 SIMARVVASSLFTLGCLAVWGIW
Inside 60-437 QWRKLQAFKSEQKREEQLRQDPIKVYEQRQEVELNQVMLNMKQSLNKHNYLYALPWYLVLGLENAGKTSLINRSGQNFVFSSVMRASGQKSENPYSFDWWIGDESVLIDPDGELLTQGNRSEENDGALERRLWLHFVDWLDRTRSRRPLNGIVLALDVAHLATATASERKAYANLLRARLRELMETLSTRLPVYIALTKLDLLHGFEPFFKHYTKSQREEVLGFTFSMDSVDNLDSWLEEFASEYTQFVSRVNGMLPHAVAAPMTLEERNAIYSFTRQISGLKEILQQFFQEALASDQFSTSALVRGAYFTSVYQQGVPTNAFDDAASRRYGLSHAINTAQRAKNSTVYFTQKLFTHIIYPEAGLASDNFRVAKNKRR
Transmembrane helix 438-457 LMGLSFVACSVATLLLAGTW
Outside 458-1166 HRNYLNNVQHADTVLTKVNQYKEQFPTSRSLASQREVLDPLNKIREATLEFGFFRDKPQYISDFGLYQGHTIGPKVEETYLNLLETRFLPLLMADTIVALNQAETDEEKLAVLRVYRMLVDKSGRYQDYVMDYFAKYWQKSFSGQRQIQEELLGHLDYAMRHTDLTAERLNGDKGAEQVMRPYDKVIARAQVELGSMPNDQRVYRNLKLSAQTVLGPSVNLRSLIGPVFDVVFEERVLNSSSLFIPQMLTKRGFDDYFMPQSESVSELALIDSWVLGQSKTAQFSEADKQALREKIRDLYVADYTNTWRAALNEIDVKYFNDINDAVMVLENITSNLEPMQRLLRTLDDNTQLYSALPKDESALKELLKSPKYKVASMIETPFADLNGMLKPVGSKPAYMTEVLASVDELKSYLKSIQDAPDVGMAALDATKARVKLVNADPIYTLKRISSGLPKPLDSMMAKLADESWYVVKQEAIKHLEVRWTEDVYKTFQSKLAGRYPFNPASNKDVALADFEAFFAPNGTLDNFYNQQLKMFIDENISVASDDSAQSIIRKEVLDQIKQAQKIREAFFNRKGILDVSFSVEPLSLSNNKRRSVLNVDGQFLAYSHGPRENVELIWPNTLRDSAVSKVTLIPTQTNMSPRSLQIQGPWAFFRLLDQGDVVSASQTSVDFKFIVDGGEMIYRINAEADANPFTERLFKSFKLSKTLY


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9975 - -


  Protein sequence: 1167 a.a.    

>T6CP013717 NZ_CP016325:131995-135495 [Vibrio cholerae 2740-80] [TssM]
VVAALPILLFTTFILLNVAIWWAGPWLEVAGYKPLESIMARVVASSLFTLGCLAVWGIWQWRKLQAFKSEQKREEQLRQD
PIKVYEQRQEVELNQVMLNMKQSLNKHNYLYALPWYLVLGLENAGKTSLINRSGQNFVFSSVMRASGQKSENPYSFDWWI
GDESVLIDPDGELLTQGNRSEENDGALERRLWLHFVDWLDRTRSRRPLNGIVLALDVAHLATATASERKAYANLLRARLR
ELMETLSTRLPVYIALTKLDLLHGFEPFFKHYTKSQREEVLGFTFSMDSVDNLDSWLEEFASEYTQFVSRVNGMLPHAVA
APMTLEERNAIYSFTRQISGLKEILQQFFQEALASDQFSTSALVRGAYFTSVYQQGVPTNAFDDAASRRYGLSHAINTAQ
RAKNSTVYFTQKLFTHIIYPEAGLASDNFRVAKNKRRLMGLSFVACSVATLLLAGTWHRNYLNNVQHADTVLTKVNQYKE
QFPTSRSLASQREVLDPLNKIREATLEFGFFRDKPQYISDFGLYQGHTIGPKVEETYLNLLETRFLPLLMADTIVALNQA
ETDEEKLAVLRVYRMLVDKSGRYQDYVMDYFAKYWQKSFSGQRQIQEELLGHLDYAMRHTDLTAERLNGDKGAEQVMRPY
DKVIARAQVELGSMPNDQRVYRNLKLSAQTVLGPSVNLRSLIGPVFDVVFEERVLNSSSLFIPQMLTKRGFDDYFMPQSE
SVSELALIDSWVLGQSKTAQFSEADKQALREKIRDLYVADYTNTWRAALNEIDVKYFNDINDAVMVLENITSNLEPMQRL
LRTLDDNTQLYSALPKDESALKELLKSPKYKVASMIETPFADLNGMLKPVGSKPAYMTEVLASVDELKSYLKSIQDAPDV
GMAALDATKARVKLVNADPIYTLKRISSGLPKPLDSMMAKLADESWYVVKQEAIKHLEVRWTEDVYKTFQSKLAGRYPFN
PASNKDVALADFEAFFAPNGTLDNFYNQQLKMFIDENISVASDDSAQSIIRKEVLDQIKQAQKIREAFFNRKGILDVSFS
VEPLSLSNNKRRSVLNVDGQFLAYSHGPRENVELIWPNTLRDSAVSKVTLIPTQTNMSPRSLQIQGPWAFFRLLDQGDVV
SASQTSVDFKFIVDGGEMIYRINAEADANPFTERLFKSFKLSKTLY*

  Nucleotide sequence: 3501 bp    

>T6CP013717 NZ_CP016325:131995-135495 [Vibrio cholerae 2740-80] [TssM]
GTGGTGGCAGCACTTCCCATTTTGCTATTCACTACGTTCATTTTGCTGAACGTTGCCATTTGGTGGGCAGGCCCTTGGTT
AGAAGTGGCTGGCTATAAACCGCTGGAGTCGATCATGGCTCGAGTTGTGGCAAGCAGTCTGTTTACTCTCGGCTGTTTAG
CCGTGTGGGGCATTTGGCAGTGGCGTAAGTTGCAAGCCTTCAAATCGGAGCAAAAGCGCGAAGAGCAACTGCGCCAAGAT
CCGATCAAGGTGTATGAGCAGCGCCAAGAGGTTGAACTGAATCAGGTGATGCTCAATATGAAGCAGAGCCTAAATAAACA
CAACTACTTGTATGCACTACCTTGGTATTTGGTTCTAGGTTTAGAAAATGCGGGTAAAACCAGCCTGATCAACCGTTCTG
GTCAGAACTTTGTCTTCTCTTCGGTCATGCGAGCTTCGGGACAAAAAAGCGAAAACCCATACTCGTTTGATTGGTGGATT
GGTGATGAATCTGTCCTGATTGATCCTGATGGTGAGCTGTTAACGCAAGGCAATCGTAGTGAAGAAAATGATGGTGCGTT
AGAGCGCCGCTTATGGTTGCATTTTGTCGATTGGCTAGACCGCACTCGTAGCCGCCGCCCGTTAAACGGCATCGTGTTGG
CGTTGGATGTGGCGCATCTTGCTACCGCCACCGCCTCAGAGCGTAAAGCTTATGCCAATTTGCTGCGTGCTCGTCTGCGT
GAGTTGATGGAAACCTTATCGACTCGTCTGCCGGTGTACATTGCACTGACTAAGCTCGACTTACTGCACGGTTTTGAGCC
TTTCTTTAAGCACTACACCAAAAGTCAGCGCGAAGAGGTGTTGGGTTTTACCTTCTCAATGGATTCGGTAGATAACCTAG
ATTCATGGTTGGAAGAGTTTGCCAGTGAGTACACTCAATTTGTGTCACGAGTTAATGGCATGCTGCCGCATGCGGTCGCA
GCGCCAATGACGCTTGAAGAACGTAACGCGATTTACAGCTTCACGCGTCAAATCTCAGGTTTGAAAGAGATTTTGCAACA
GTTTTTCCAAGAGGCTTTGGCGAGCGATCAATTCTCAACGTCAGCGCTGGTGCGTGGTGCCTATTTCACGTCGGTTTACC
AACAAGGTGTACCTACCAACGCTTTTGATGATGCGGCTTCCCGTCGTTATGGTCTGTCTCATGCGATTAATACGGCGCAG
CGCGCGAAAAACTCCACGGTCTATTTCACGCAAAAACTGTTTACCCACATCATTTATCCTGAAGCGGGTTTAGCGTCGGA
TAATTTCCGGGTTGCAAAAAACAAACGCCGTTTGATGGGGCTCTCTTTTGTCGCATGCTCAGTGGCGACTTTGCTGTTGG
CGGGAACTTGGCATCGTAACTACCTCAATAACGTGCAACACGCCGATACTGTATTAACCAAAGTGAATCAGTATAAAGAG
CAGTTCCCGACCAGCCGTTCATTGGCCTCACAAAGAGAGGTATTGGATCCGTTGAACAAGATCCGTGAGGCGACACTGGA
GTTTGGTTTCTTCCGTGATAAACCTCAGTACATCTCCGATTTTGGTCTCTACCAAGGGCACACCATTGGCCCGAAAGTAG
AAGAGACCTACTTAAACTTACTCGAAACTCGCTTCTTGCCTCTGTTGATGGCGGACACCATTGTTGCGCTCAATCAAGCT
GAGACAGATGAAGAAAAATTAGCGGTGCTGCGTGTGTATCGCATGCTGGTCGATAAAAGTGGTCGTTACCAAGACTATGT
GATGGATTATTTTGCGAAGTATTGGCAAAAATCCTTCTCAGGTCAGCGTCAAATTCAGGAAGAGCTGCTCGGCCACTTAG
ATTACGCGATGCGTCATACGGACCTCACTGCAGAGAGGCTCAATGGTGATAAAGGGGCGGAGCAGGTGATGCGCCCTTAC
GATAAAGTGATTGCTCGTGCTCAGGTTGAACTGGGTTCAATGCCAAACGACCAGCGCGTCTATCGTAACTTGAAATTGAG
CGCACAAACGGTGCTTGGTCCTTCGGTTAACTTGCGTAGCTTGATTGGGCCGGTGTTCGATGTGGTGTTTGAAGAGCGCG
TTCTTAACAGTAGCAGCCTGTTTATTCCTCAAATGCTCACCAAGCGTGGTTTTGATGACTACTTTATGCCGCAATCTGAA
TCGGTCTCTGAGCTGGCACTGATTGACAGTTGGGTTCTGGGACAGAGCAAAACGGCACAGTTTAGTGAAGCAGACAAGCA
GGCACTGCGCGAAAAAATCCGCGATCTTTATGTGGCGGATTACACCAACACATGGCGCGCGGCACTCAATGAAATTGATG
TGAAGTATTTCAATGACATCAATGATGCGGTCATGGTTTTGGAAAATATCACCAGTAACCTTGAGCCGATGCAGCGTCTA
CTGCGCACATTAGATGACAACACCCAACTGTACAGTGCGTTACCTAAAGATGAATCGGCGTTAAAAGAGCTGCTGAAAAG
TCCGAAATACAAAGTGGCTTCGATGATTGAAACTCCGTTTGCTGATCTGAACGGTATGCTCAAACCGGTGGGCAGCAAAC
CAGCGTACATGACGGAAGTCTTGGCTTCGGTGGATGAGCTGAAAAGCTACTTGAAATCGATTCAAGATGCTCCCGATGTC
GGTATGGCGGCGCTCGATGCGACCAAAGCGCGGGTCAAACTGGTTAATGCTGACCCTATCTATACCTTAAAACGTATCTC
GTCAGGACTGCCGAAGCCGCTGGATAGCATGATGGCAAAGCTTGCAGATGAGAGCTGGTATGTGGTCAAGCAAGAAGCGA
TCAAGCATCTGGAAGTTCGCTGGACGGAGGATGTGTATAAAACCTTCCAAAGCAAGCTGGCTGGGCGTTATCCATTCAAC
CCCGCATCAAATAAAGATGTTGCCTTGGCCGATTTTGAAGCCTTCTTTGCGCCGAATGGCACGTTAGACAACTTCTACAA
TCAGCAACTGAAGATGTTTATTGATGAGAACATCAGCGTCGCGTCGGATGACTCAGCGCAGTCGATCATTCGTAAAGAAG
TGCTGGATCAGATCAAGCAAGCGCAGAAAATTCGTGAGGCTTTCTTCAACCGTAAAGGGATTTTGGATGTCAGCTTCTCG
GTTGAGCCGCTCAGTTTGAGTAACAACAAGCGTCGTAGTGTGCTGAATGTGGATGGTCAGTTCTTAGCTTACAGCCATGG
TCCGCGTGAAAATGTGGAGCTGATTTGGCCTAACACGCTGCGTGATTCTGCGGTGTCTAAGGTGACCTTGATTCCAACCC
AAACCAATATGTCACCGCGCAGTTTGCAAATCCAAGGACCGTGGGCATTCTTCCGCTTATTGGATCAAGGTGATGTGGTT
TCGGCGAGTCAAACGTCGGTTGATTTCAAATTTATCGTCGATGGCGGTGAGATGATCTACCGTATTAACGCAGAGGCCGA
TGCCAACCCATTTACCGAACGCCTGTTCAAGTCGTTCAAACTGTCTAAAACACTCTATTAA