Detailed information of component protein


Summary

Component ID T6CP013400
Component protein type TssM
T6SS ID (Type) T6SS01033 experimental (Type i3)
Strain Serratia sp. FS14
Replicon chromosome
Sequence Protein sequence (1160 a.a.); Nucleotide sequence (3480 bp)
Reference
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) L085_RS12945
Coordinate (Strand) 2850755..2854234 (+)
NCBI ID WP_044030913.1
RefSeq NZ_CP005927
Uniprot ID UPI0004994631
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related PF06761.14 2.2e-59 515..793
IcmF-related_N PF14331.8 8.9e-30 197..367
IcmF_C PF06744.14 1.7e-23 1035..1131

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-4 MIRR
Transmembrane helix 5-27 SFILVIALLLCAVVWWIGPLIAI
Outside 28-41 GTYRPLLSVWVRGI
Transmembrane helix 42-64 IIALILCWALWPFVATFLSWVFR
Inside 65-430 HARAPAPAKRKTAQRDRVTSRFYDALRTLQYVGTAKQRTPWQRFKYRLARRYVSEKPWFLVIGPPGSGKTSLIGESGERFLLAEHYGLQQTVDVGPTQDCNWWLAENSVYVDTSGEWLQLNGLGEEGARARDTLFKLIRRHRRHPGIDGIMLCLDARWLLHASLTERKSVADALRVRLLEMASWFRSELPVYLTISHLDQLVGGETFLSMLGDDLLQKGMGFSLNRAEQGETPFEQYETLYREMVARVSHHVLELLHDAPDGQNRQQLLFFTESLGSLGNPLYSLLEQIFPQTPVGYACHLQQVWLGSTIALPPQGAIYNAEAADIYEARPTGRIYHPELDYARQERGVMQQSSHARPLGSRMSSL
Transmembrane helix 431-453 GRYVLVLILLAVSFNLLAMRYLW
Outside 454-1159 ESDYISYIAARFDETKRIVREIPVTNRVSDDVISAYEQLGYMNAQLGGEVSPVPNPYFEHKLINSAAQKTYQRHLLQIFWPAVENYIADELKNDVSASNKDVYGTLKVYLMMGEPSHRSAEALVDWFMARWETFMPQGYTDMNKGIFAYHLREMFSLPDAPVMKMNGDLLRLARVKAMNIPMQVRVVRRIEEKPLPAAINDISLADAAGPNVSLMLRRKSQATVTDTAVPGFYTRASYRDVFLAQLDDAAKDMIEEESWVLRDSGEGKNAVNSLASAQKLADEARKLYLIEYADKWDAFMRDVRARPVNGLEDAAILARQLSDPSFPLANLVRFAARETSMTGTNQGDAASWFDRQRNRIEQQRRDILGEISGERARFRLTPERAVEDRFELLRRLGYQLLQTTNASNDPLSRSFEALYSQLTTLSTSLRGGQVVPAGGTLKRLQLDAARQPEPVRSVMMDLLQVGDSQTIQQSQKNLSKGASSLASGLCKSSISGRYPFSRNARAEVGIEDFSRMFGQSGAMQQFFDENLAPYVDVNAQKWNAKPGSEGIVNASTLGAFENARLIRDTFFTAGDKLSFSMFIRPLSLTPTITEATLDIDGQVINYSHGFSQPTRIDWPGPKGGSYVRLTFRTGTGQIQVASFDGPWALFRMFDASNPVPLASDRRELTMSMSSVVGVLKLELRSTMKDFPLWSRALRGFSCPKAM



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9598 - -



  Protein sequence: 1160 a.a.    

>T6CP013400 NZ_CP005927:2850755-2854234 [Serratia sp. FS14] [TssM]
MIRRSFILVIALLLCAVVWWIGPLIAIGTYRPLLSVWVRGIIIALILCWALWPFVATFLSWVFRHARAPAPAKRKTAQRD
RVTSRFYDALRTLQYVGTAKQRTPWQRFKYRLARRYVSEKPWFLVIGPPGSGKTSLIGESGERFLLAEHYGLQQTVDVGP
TQDCNWWLAENSVYVDTSGEWLQLNGLGEEGARARDTLFKLIRRHRRHPGIDGIMLCLDARWLLHASLTERKSVADALRV
RLLEMASWFRSELPVYLTISHLDQLVGGETFLSMLGDDLLQKGMGFSLNRAEQGETPFEQYETLYREMVARVSHHVLELL
HDAPDGQNRQQLLFFTESLGSLGNPLYSLLEQIFPQTPVGYACHLQQVWLGSTIALPPQGAIYNAEAADIYEARPTGRIY
HPELDYARQERGVMQQSSHARPLGSRMSSLGRYVLVLILLAVSFNLLAMRYLWESDYISYIAARFDETKRIVREIPVTNR
VSDDVISAYEQLGYMNAQLGGEVSPVPNPYFEHKLINSAAQKTYQRHLLQIFWPAVENYIADELKNDVSASNKDVYGTLK
VYLMMGEPSHRSAEALVDWFMARWETFMPQGYTDMNKGIFAYHLREMFSLPDAPVMKMNGDLLRLARVKAMNIPMQVRVV
RRIEEKPLPAAINDISLADAAGPNVSLMLRRKSQATVTDTAVPGFYTRASYRDVFLAQLDDAAKDMIEEESWVLRDSGEG
KNAVNSLASAQKLADEARKLYLIEYADKWDAFMRDVRARPVNGLEDAAILARQLSDPSFPLANLVRFAARETSMTGTNQG
DAASWFDRQRNRIEQQRRDILGEISGERARFRLTPERAVEDRFELLRRLGYQLLQTTNASNDPLSRSFEALYSQLTTLST
SLRGGQVVPAGGTLKRLQLDAARQPEPVRSVMMDLLQVGDSQTIQQSQKNLSKGASSLASGLCKSSISGRYPFSRNARAE
VGIEDFSRMFGQSGAMQQFFDENLAPYVDVNAQKWNAKPGSEGIVNASTLGAFENARLIRDTFFTAGDKLSFSMFIRPLS
LTPTITEATLDIDGQVINYSHGFSQPTRIDWPGPKGGSYVRLTFRTGTGQIQVASFDGPWALFRMFDASNPVPLASDRRE
LTMSMSSVVGVLKLELRSTMKDFPLWSRALRGFSCPKAM*

  Nucleotide sequence: 3480 bp    

>T6CP013400 NZ_CP005927:2850755-2854234 [Serratia sp. FS14] [TssM]
ATGATACGTAGATCATTCATATTGGTGATTGCCTTGCTGCTGTGTGCGGTGGTGTGGTGGATTGGGCCGTTGATCGCCAT
CGGCACCTATCGGCCGCTGCTGTCGGTCTGGGTCAGGGGCATCATCATCGCGCTGATCCTGTGCTGGGCGCTGTGGCCGT
TCGTGGCGACGTTCTTATCCTGGGTGTTCCGCCACGCGCGAGCGCCGGCCCCGGCCAAACGCAAGACCGCCCAGCGAGAT
CGCGTGACCTCGCGCTTTTACGACGCCTTGCGCACCTTGCAGTACGTCGGCACGGCGAAGCAGCGCACCCCCTGGCAGCG
CTTCAAATACCGCCTGGCGCGCCGTTACGTCAGCGAAAAGCCGTGGTTTCTGGTCATCGGGCCGCCGGGCAGCGGCAAAA
CCTCGCTGATCGGCGAAAGCGGCGAACGCTTCTTGCTGGCCGAGCACTATGGACTGCAGCAGACGGTGGACGTCGGGCCG
ACCCAGGATTGCAACTGGTGGCTGGCGGAGAATTCGGTGTATGTCGACACCTCCGGCGAATGGCTGCAGTTGAACGGCCT
GGGGGAAGAGGGCGCCAGGGCGCGCGACACCCTGTTCAAACTGATCCGCCGCCATCGCCGCCATCCGGGCATCGACGGCA
TCATGCTGTGTCTGGATGCGCGTTGGTTGCTGCACGCCTCGCTGACGGAGAGAAAGTCGGTCGCCGACGCGCTGCGCGTG
CGCCTGCTGGAGATGGCCTCGTGGTTCCGCAGCGAGCTGCCGGTCTATCTTACCATCAGCCACCTCGATCAACTGGTCGG
CGGCGAGACCTTCCTGTCGATGCTGGGCGACGATCTGTTGCAAAAAGGCATGGGCTTCAGCCTGAACCGCGCCGAACAGG
GAGAAACCCCCTTCGAGCAGTATGAAACCCTCTACCGGGAGATGGTGGCGCGCGTCAGCCATCACGTACTCGAACTGTTG
CACGATGCGCCGGACGGCCAAAACCGCCAGCAGCTGCTGTTCTTCACCGAGTCGCTGGGGTCGCTGGGCAACCCGCTGTA
TTCCCTGTTAGAGCAGATCTTCCCGCAAACGCCGGTGGGCTACGCCTGCCATCTGCAGCAGGTCTGGCTGGGCAGCACCA
TCGCCTTGCCGCCGCAGGGGGCGATCTACAACGCGGAAGCGGCGGATATCTATGAAGCTCGCCCGACCGGGCGCATTTAT
CACCCGGAGCTGGATTACGCCCGGCAAGAACGCGGGGTGATGCAGCAGTCTTCGCACGCCAGGCCGCTCGGCAGCCGCAT
GAGCTCCCTGGGCCGCTATGTGCTGGTGCTCATTCTGTTGGCGGTCTCGTTTAACCTGCTGGCGATGCGTTACCTGTGGG
AGAGCGATTACATCTCCTATATTGCGGCGCGCTTCGATGAGACAAAGCGCATCGTGCGGGAAATTCCGGTGACTAACCGC
GTCAGCGACGATGTCATTTCCGCCTATGAGCAGCTGGGCTACATGAACGCGCAATTGGGCGGCGAAGTGTCGCCGGTGCC
GAATCCCTATTTCGAGCACAAGCTGATCAACAGCGCGGCGCAAAAAACCTACCAACGCCATTTGCTGCAGATCTTCTGGC
CGGCGGTGGAAAACTATATCGCTGACGAACTCAAGAATGACGTCTCGGCGTCCAACAAGGACGTCTACGGCACCCTGAAA
GTGTACCTGATGATGGGGGAACCCTCGCACCGCTCCGCCGAAGCCTTGGTCGACTGGTTCATGGCGCGCTGGGAAACCTT
TATGCCGCAGGGGTACACCGACATGAACAAAGGCATCTTCGCTTACCATCTGCGGGAGATGTTCTCTTTGCCGGACGCGC
CGGTCATGAAGATGAACGGCGATCTGCTGCGCCTGGCGCGGGTGAAAGCGATGAACATTCCGATGCAGGTGCGGGTGGTG
CGCCGCATCGAAGAGAAACCGCTGCCGGCGGCGATCAACGATATTTCGTTGGCCGACGCCGCCGGGCCGAACGTCTCCTT
GATGCTGCGCCGCAAGAGCCAGGCGACGGTCACCGATACCGCCGTGCCGGGGTTCTATACCCGCGCCAGCTATCGCGACG
TTTTCCTGGCGCAGCTCGATGACGCCGCCAAGGACATGATTGAAGAAGAGTCCTGGGTGCTGCGCGACAGTGGGGAAGGG
AAAAACGCGGTGAACTCGCTGGCCTCGGCGCAGAAGCTGGCCGACGAGGCGAGGAAGCTTTACCTCATCGAGTATGCCGA
CAAATGGGATGCGTTCATGCGCGACGTGCGCGCCCGGCCGGTCAACGGGCTTGAGGATGCGGCGATCCTGGCGCGTCAAC
TGTCCGACCCTTCTTTCCCGCTGGCCAATCTGGTGCGTTTCGCCGCGCGCGAAACCAGCATGACCGGCACCAACCAGGGC
GATGCGGCAAGCTGGTTCGATCGCCAGCGCAACCGCATCGAGCAGCAGCGGCGCGACATCCTCGGCGAGATTTCCGGTGA
GCGCGCGCGTTTTCGTCTCACGCCGGAACGCGCGGTGGAAGATCGCTTCGAGCTGCTGCGGCGTCTGGGCTATCAGCTGC
TGCAGACCACCAACGCCAGCAACGATCCGCTGAGCCGCAGTTTCGAAGCGCTGTACAGCCAGTTGACCACCTTGTCCACC
TCGCTGCGCGGCGGCCAGGTGGTGCCCGCCGGAGGCACGCTGAAGCGTCTGCAGCTGGATGCGGCGCGCCAGCCGGAACC
GGTGCGCAGCGTGATGATGGACTTGTTGCAGGTCGGGGACAGCCAGACGATTCAGCAGAGCCAAAAGAACCTGAGCAAAG
GTGCTTCGTCGCTGGCCTCCGGCTTGTGCAAGAGCAGCATCAGCGGGCGTTATCCATTCTCGCGCAACGCCAGAGCGGAG
GTCGGCATCGAGGACTTCTCGCGCATGTTTGGCCAAAGCGGCGCGATGCAGCAGTTCTTCGATGAGAACCTGGCCCCTTA
TGTGGACGTCAACGCGCAGAAATGGAATGCCAAACCGGGTTCAGAGGGGATCGTCAATGCCAGCACGCTGGGCGCGTTTG
AAAATGCCCGCCTGATCCGCGATACCTTCTTCACTGCCGGCGACAAGCTGTCTTTCTCCATGTTCATCAGGCCGTTGTCC
CTGACGCCGACCATTACGGAAGCGACCCTGGATATCGACGGGCAGGTGATTAACTACAGCCATGGTTTCAGCCAGCCTAC
CCGTATCGATTGGCCGGGCCCGAAAGGCGGCAGCTATGTTCGACTCACCTTCCGCACCGGCACCGGCCAGATCCAGGTCG
CCAGTTTCGACGGGCCCTGGGCCTTGTTCCGGATGTTCGACGCCAGCAATCCCGTGCCGCTCGCCAGCGACAGGCGAGAG
CTGACGATGTCGATGTCGTCGGTGGTGGGCGTGCTCAAACTGGAATTGCGCTCGACGATGAAAGATTTCCCACTTTGGTC
ACGGGCGTTGCGAGGATTCTCGTGCCCGAAAGCGATGTAA