Detailed information of component protein


Summary

Component ID T6CP013399
Component protein type TssL
T6SS ID (Type) T6SS01033 experimental (Type i3)
Strain Serratia sp. FS14
Replicon chromosome
Sequence Protein sequence (504 a.a.); Nucleotide sequence (1512 bp)
Reference
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) L085_RS12940
Coordinate (Strand) 2849208..2850719 (+)
NCBI ID WP_044030912.1
RefSeq NZ_CP005927
Uniprot ID UPI000499431E
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 1e-37 113..302
OmpA PF00691.22 9.9e-11 368..470

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-288 MNEFERKIREAMAVTRNNATPRLPASGSVGKTTAEIAALSFGARALAAKLRPGSAGLATPPEPKPYEAESVAPESSEPAPGERPAEAAPTSSGLFGRAALSKPGVMMGHWDNPLIAAAIPVLLQVERVRDQKQLNPGAVRAQIVREIQQFQQTLLADHIAPSDVDKLAYLLCSYVDECCMSTLESGELNLSLLVEFYRDAWGGEKCFEHLQAYLAASPRNKEILAFYDLILSLGFEGQYHVMERGAVLLADVRNRLDGYLYSQNPTQTLADAEPSKVVRKATRITPLK
Transmembrane helix 289-308 LLCYGLAIALLAYASAAWYL
Outside 309-503 HDQSRALRNAILAWTPPQPRKINIMETLPQPLPQILSEGWLEVRADPRGWLLIFTSDGAFATGQSKLSEEFKKKRNIERLGEALAPWPGDLEVIGHTDNKPYRNTSNNSNLKLSEARAQTVADKLRESLANSKYNRTVTSVGKGDQEPLDDNATEEGRRRNRRVDILWKIGEREMASPSATLNQAGVAAEVPEDN



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.6040 - -



  Protein sequence: 504 a.a.    

>T6CP013399 NZ_CP005927:2849208-2850719 [Serratia sp. FS14] [TssL]
MNEFERKIREAMAVTRNNATPRLPASGSVGKTTAEIAALSFGARALAAKLRPGSAGLATPPEPKPYEAESVAPESSEPAP
GERPAEAAPTSSGLFGRAALSKPGVMMGHWDNPLIAAAIPVLLQVERVRDQKQLNPGAVRAQIVREIQQFQQTLLADHIA
PSDVDKLAYLLCSYVDECCMSTLESGELNLSLLVEFYRDAWGGEKCFEHLQAYLAASPRNKEILAFYDLILSLGFEGQYH
VMERGAVLLADVRNRLDGYLYSQNPTQTLADAEPSKVVRKATRITPLKLLCYGLAIALLAYASAAWYLHDQSRALRNAIL
AWTPPQPRKINIMETLPQPLPQILSEGWLEVRADPRGWLLIFTSDGAFATGQSKLSEEFKKKRNIERLGEALAPWPGDLE
VIGHTDNKPYRNTSNNSNLKLSEARAQTVADKLRESLANSKYNRTVTSVGKGDQEPLDDNATEEGRRRNRRVDILWKIGE
REMASPSATLNQAGVAAEVPEDN*

  Nucleotide sequence: 1512 bp    

>T6CP013399 NZ_CP005927:2849208-2850719 [Serratia sp. FS14] [TssL]
ATGAACGAATTCGAGAGAAAAATACGCGAGGCGATGGCGGTAACGCGCAACAACGCGACGCCGCGCCTTCCCGCGTCCGG
CTCGGTGGGCAAAACGACGGCGGAGATCGCCGCGCTGTCCTTCGGCGCGCGGGCGCTGGCCGCCAAGCTGCGGCCGGGAA
GCGCCGGGCTGGCGACGCCCCCCGAACCCAAGCCTTATGAAGCGGAAAGCGTTGCGCCGGAAAGCAGCGAGCCCGCGCCG
GGCGAGCGCCCGGCCGAGGCGGCGCCGACCTCGAGCGGGCTGTTTGGCCGCGCCGCGCTGAGCAAGCCCGGCGTGATGAT
GGGCCATTGGGACAACCCGCTGATCGCCGCCGCCATCCCGGTCCTGCTGCAGGTGGAGCGGGTGCGCGATCAGAAACAGC
TGAATCCGGGCGCGGTGCGCGCGCAGATCGTGCGCGAGATCCAACAATTTCAGCAGACGCTGCTGGCGGATCACATCGCG
CCGAGCGATGTGGACAAGCTGGCCTATCTGCTGTGCTCCTACGTGGATGAGTGCTGCATGAGCACGCTCGAATCCGGCGA
ACTGAATCTCAGCCTGCTGGTGGAGTTTTATCGCGACGCCTGGGGCGGGGAAAAGTGCTTCGAGCATCTGCAGGCCTATC
TGGCCGCCTCGCCGCGCAACAAGGAGATCCTGGCGTTTTACGATCTGATCCTGTCGCTGGGCTTCGAAGGGCAATATCAC
GTGATGGAACGCGGCGCGGTGTTGTTGGCCGACGTGCGCAACCGTCTTGACGGCTATCTGTACAGCCAGAACCCGACGCA
GACGCTGGCCGATGCCGAGCCGAGCAAAGTGGTGCGCAAGGCGACCCGCATCACGCCGCTGAAGCTGCTGTGCTACGGCC
TGGCCATCGCGCTGCTGGCGTATGCCTCGGCCGCCTGGTATCTGCACGATCAGTCCCGCGCGCTGCGCAACGCCATTTTG
GCCTGGACGCCGCCGCAGCCGCGCAAAATCAACATCATGGAGACGCTGCCGCAGCCGCTGCCGCAAATCCTCAGCGAAGG
GTGGCTGGAGGTGCGCGCCGATCCGCGCGGCTGGCTGTTGATCTTCACCTCCGACGGCGCGTTCGCCACCGGGCAGTCGA
AGCTGTCGGAAGAGTTTAAAAAGAAACGCAACATCGAGCGTTTGGGGGAAGCGCTGGCGCCGTGGCCGGGCGATCTGGAG
GTCATAGGGCATACCGACAACAAACCGTATCGCAACACCTCCAACAACTCCAACCTGAAACTGTCCGAGGCGCGCGCGCA
GACGGTGGCCGACAAGCTGCGCGAATCCTTGGCCAACAGTAAATACAACCGCACCGTGACATCGGTCGGCAAGGGCGATC
AGGAGCCGCTGGACGACAACGCGACGGAAGAAGGGCGCCGCCGCAACCGCCGCGTGGACATCCTTTGGAAAATCGGCGAG
AGGGAGATGGCTTCACCGTCTGCAACGTTAAATCAGGCAGGGGTAGCCGCCGAGGTCCCCGAGGATAACTAA