SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP013397
Component protein type	TssJ
T6SS ID (Type)	T6SS01033 (Type i3)
Strain	Serratia sp. FS14
Replicon	chromosome
Sequence	Protein sequence (169 a.a.); Nucleotide sequence (507 bp)
Reference	[1] Li P, Xu D, Ma T, et al. Crystal structures of the kinase domain of PpkA, a key regulatory component of T6SS, reveal a general inhibitory mechanism. Biochem J. 2018 Jul 17;475(13):2209-2224. doi: 10.1042/BCJ20180077. PMID: 29858276

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	L085_RS12930
Coordinate (Strand)	2847345..2847851 (+)
NCBI ID	WP_004935335.1
RefSeq	NZ_CP005927
Uniprot ID	UPI0002B89ACF
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	8.5e-10	40..153

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-4	MVKT
Transmembrane helix	5-27	LRGVAAGGLLAVGMLGGCSWFSH
Outside	28-168	DEAADAGRRLRFIDVEVTANDNVNPNAAGEAQPVKVCVIETNRSGWMPEGIGDGAPCRETAPSEGALSRHQSILQPRETLRYRFEVPAGQERWVVVGAEFQQISGVLPLAEQKSPAQADSQMKVMVGMTSLSIVTNDKMDE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9973	pos: 21-22. LGG-CS.	MVKTLRGVAAGGLLAVGMLGG

Protein sequence: 169 a.a.

						>T6CP013397 NZ_CP005927:2847345-2847851 [Serratia sp. FS14] [TssJ]
MVKTLRGVAAGGLLAVGMLGGCSWFSHDEAADAGRRLRFIDVEVTANDNVNPNAAGEAQPVKVCVIETNRSGWMPEGIGD
GAPCRETAPSEGALSRHQSILQPRETLRYRFEVPAGQERWVVVGAEFQQISGVLPLAEQKSPAQADSQMKVMVGMTSLSI
VTNDKMDE*					

Nucleotide sequence: 507 bp

						>T6CP013397 NZ_CP005927:2847345-2847851 [Serratia sp. FS14] [TssJ]
ATGGTGAAGACGCTTCGGGGAGTCGCGGCAGGGGGATTGTTGGCGGTGGGGATGCTCGGCGGATGCAGCTGGTTTTCTCA
CGACGAGGCAGCGGACGCGGGGCGTCGGTTGCGGTTTATCGACGTCGAAGTGACCGCCAACGACAACGTGAACCCGAATG
CGGCCGGCGAGGCGCAACCGGTCAAGGTATGCGTGATAGAAACCAATCGCTCGGGGTGGATGCCCGAGGGGATTGGCGAC
GGCGCTCCTTGCCGCGAGACGGCGCCGTCGGAAGGGGCGCTGAGCCGGCACCAGAGCATTTTGCAACCGAGGGAGACGTT
GCGTTATCGCTTCGAGGTGCCGGCGGGGCAAGAGCGATGGGTCGTCGTCGGGGCGGAGTTTCAGCAAATCAGCGGCGTGT
TGCCGCTCGCCGAGCAAAAGTCCCCTGCGCAGGCTGATTCGCAAATGAAGGTGATGGTGGGGATGACGTCACTCAGCATA
GTGACTAACGACAAAATGGATGAATGA					