SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP013394
Component protein type	TssG
T6SS ID (Type)	T6SS01033 (Type i3)
Strain	Serratia sp. FS14
Replicon	chromosome
Sequence	Protein sequence (343 a.a.); Nucleotide sequence (1029 bp)
Reference	[1] Li P, Xu D, Ma T, et al. Crystal structures of the kinase domain of PpkA, a key regulatory component of T6SS, reveal a general inhibitory mechanism. Biochem J. 2018 Jul 17;475(13):2209-2224. doi: 10.1042/BCJ20180077. PMID: 29858276

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	L085_RS12895
Coordinate (Strand)	2837018..2838046 (+)
NCBI ID	WP_004935359.1
RefSeq	NZ_CP005927
Uniprot ID	A0A6G8TPN9_SERMA
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	1.3e-54	38..327

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-342	MADMSLMRETALSLEQAPTPGAQSESAGQAEIRGLSNRQDFFELLRRIERAQPDAPRLGVSNDRSRERLCITQPADMAFASREIASIVQRPKQVLIQARHFGMFAPYGPLPIHVTEHARTEVIARRNQAFQQFVGIVSQRFAVLHYRAWAQLKAMIGHEHDDDKNPFLRHLRQTVGVDTALTVNPHIQRLREAYPGTYLPGRRSLRQLNKMLAAYFAVPITITPRYAKWIDDGKQSGSQKLGKLGSTRIGSRFFDAQYGAHIQIGPLGAPQYQQYQRGSRRLQALVSICHDFTSHQLMLDVSLLIATEPGMAAQLGGKSLSKDSWLKPKTGTFRQLVYQSTT

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9810	-	-

Protein sequence: 343 a.a.

						>T6CP013394 NZ_CP005927:2837018-2838046 [Serratia sp. FS14] [TssG]
MADMSLMRETALSLEQAPTPGAQSESAGQAEIRGLSNRQDFFELLRRIERAQPDAPRLGVSNDRSRERLCITQPADMAFA
SREIASIVQRPKQVLIQARHFGMFAPYGPLPIHVTEHARTEVIARRNQAFQQFVGIVSQRFAVLHYRAWAQLKAMIGHEH
DDDKNPFLRHLRQTVGVDTALTVNPHIQRLREAYPGTYLPGRRSLRQLNKMLAAYFAVPITITPRYAKWIDDGKQSGSQK
LGKLGSTRIGSRFFDAQYGAHIQIGPLGAPQYQQYQRGSRRLQALVSICHDFTSHQLMLDVSLLIATEPGMAAQLGGKSL
SKDSWLKPKTGTFRQLVYQSTT*					

Nucleotide sequence: 1029 bp

						>T6CP013394 NZ_CP005927:2837018-2838046 [Serratia sp. FS14] [TssG]
ATGGCTGATATGTCGTTGATGCGTGAAACGGCGCTCTCGTTGGAACAGGCGCCGACGCCGGGGGCGCAAAGCGAAAGCGC
GGGCCAGGCCGAAATCCGCGGGCTGTCCAATCGACAGGATTTTTTCGAGCTGCTGCGGCGTATCGAACGCGCGCAGCCGG
ATGCGCCGCGGCTCGGCGTCTCCAACGATCGCAGCCGCGAAAGGCTCTGCATTACCCAGCCGGCGGATATGGCGTTCGCT
TCGCGTGAAATCGCCTCTATCGTGCAGCGCCCTAAGCAAGTGCTGATTCAGGCGCGGCATTTCGGCATGTTCGCCCCCTA
CGGCCCGCTGCCGATCCATGTGACCGAGCACGCGCGCACCGAAGTCATCGCCAGACGCAACCAGGCGTTCCAGCAGTTCG
TCGGCATCGTCAGCCAGCGCTTCGCCGTATTGCACTACCGGGCCTGGGCGCAGCTGAAGGCGATGATCGGCCATGAGCAT
GACGATGACAAAAACCCGTTCTTGCGCCATTTGCGCCAGACGGTGGGCGTCGATACGGCGCTGACGGTGAACCCGCATAT
CCAGCGCCTGCGCGAGGCGTACCCCGGCACTTATCTGCCCGGTCGCCGCTCGCTGCGGCAGCTCAACAAGATGTTGGCGG
CCTATTTCGCCGTTCCCATCACGATTACGCCGCGCTACGCCAAGTGGATCGACGACGGCAAGCAAAGCGGCAGCCAAAAG
CTGGGCAAGTTGGGCAGCACGCGCATCGGCAGCCGCTTTTTCGATGCCCAGTACGGCGCGCATATTCAGATCGGCCCGCT
GGGGGCCCCGCAGTATCAGCAGTATCAGCGCGGCAGCCGGCGGCTGCAGGCGCTGGTGAGCATCTGTCATGACTTTACGA
GCCATCAGCTGATGCTGGATGTCAGCCTGCTGATCGCCACGGAGCCGGGCATGGCGGCGCAGCTGGGCGGCAAGTCGCTG
AGTAAAGACAGCTGGCTGAAACCTAAGACAGGGACGTTTAGGCAGCTGGTTTATCAATCAACAACTTAA					